Small ribosomal subunit protein uS10

UniProt P0A7R5 Organism Escherichia coli (strain K12) Gene JW3283, b3321, nusE, rpsJ

Also known as: JW3283, b3321, nusE, rpsJ

Function

Involved in the binding of tRNA to the ribosomes. Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNA polymerase (RNAP). It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis. In complex with NusB is involved in the regulation of ribosomal RNA (rRNA) biosynthesis by transcriptional antitermination. S10 binds RNA non-specifically and increases the affinity of NusB for the boxA RNA sequence. S10 may constitute the critical antitermination component of the NusB-S10 complex.

Classification

Family (Pfam): PF00338 Ribosomal_S10
InterPro: Ribosomal_uS10, Ribosomal_uS10_CS, Ribosomal_uS10_dom, Ribosomal_uS10_dom_sf
Functional cluster: Ribosomal Protein S10 & related

Experimental structures · PDB · 521

2KVQ NMR
2YKR EM 9.80A
3D3B X-ray 1.30A
3D3C X-ray 2.60A
3IMQ X-ray 2.50A
3J9Y EM 3.90A
3J9Z EM 3.60A
3JA1 EM 3.60A
3JBU EM 3.64A
3JBV EM 3.32A
3JCD EM 3.70A
3JCE EM 3.20A
… and 509 more

A predicted model is available from AlphaFold.

Gene Ontology · 12

Drugs targeting this protein · 62

TELITHROMYCIN inhibitor
DEMECLOCYCLINE HYDROCHLORIDE inhibitor
ERYTHROMYCIN LACTOBIONATE inhibitor
ERYTHROMYCIN STEARATE inhibitor
CLINDAMYCIN HYDROCHLORIDE inhibitor
CLINDAMYCIN PALMITATE HYDROCHLORIDE inhibitor
QUINUPRISTIN inhibitor
ERYTHROMYCIN ETHYLSUCCINATE inhibitor
DOXYCYCLINE inhibitor
CHLORAMPHENICOL SODIUM SUCCINATE inhibitor
MINOCYCLINE HYDROCHLORIDE inhibitor
DALFOPRISTIN inhibitor
TETRACYCLINE PHOSPHATE COMPLEX inhibitor
DIRITHROMYCIN inhibitor
TEDIZOLID inhibitor
LINEZOLID inhibitor
CHLORAMPHENICOL inhibitor
TETRACYCLINE inhibitor
KANAMYCIN SULFATE inhibitor
LINCOMYCIN inhibitor
OXYTETRACYCLINE inhibitor
OXYTETRACYCLINE HYDROCHLORIDE inhibitor
DELPAZOLID inhibitor
CLARITHROMYCIN inhibitor
BEKANAMYCIN inhibitor
ERAVACYCLINE inhibitor
OMADACYCLINE TOSYLATE inhibitor
TYLVALOSIN TARTRATE inhibitor
TEDIZOLID PHOSPHATE inhibitor
GAMITHROMYCIN inhibitor
CHLORTETRACYCLINE HYDROCHLORIDE inhibitor
METHACYCLINE HYDROCHLORIDE inhibitor
PAROMOMYCIN SULFATE inhibitor
SISOMICIN inhibitor
ERYTHROMYCIN ESTOLATE inhibitor
ERAVACYCLINE DIHYDROCHLORIDE inhibitor
JOSAMYCIN inhibitor
PLAZOMICIN SULFATE inhibitor
MECLOCYCLINE SULFOSALICYLATE inhibitor
TILDIPIROSIN inhibitor
QUINUPRISTIN/DALFOPRISTIN inhibitor
CLINDAMYCIN PHOSPHATE inhibitor
STREPTOMYCIN SULFATE inhibitor
DIHYDROSTREPTOMYCIN SULFATE inhibitor
CONTEZOLID inhibitor
ERYTHROMYCIN GLUCEPTATE inhibitor
LEFAMULIN ACETATE inhibitor
CADAZOLID inhibitor
TIGECYCLINE inhibitor
OXYTETRACYCLINE CALCIUM inhibitor
SPECTINOMYCIN HYDROCHLORIDE inhibitor
DOXYCYCLINE HYCLATE inhibitor
NETILMICIN SULFATE inhibitor
PIRLIMYCIN HYDROCHLORIDE inhibitor
CETHROMYCIN inhibitor
CONTEZOLID ACEFOSAMIL inhibitor
AMIKACIN SULFATE inhibitor
NAFITHROMYCIN inhibitor
TETRACYCLINE HYDROCHLORIDE inhibitor
RADEZOLID inhibitor
AZITHROMYCIN inhibitor
ERYTHROMYCIN inhibitor

Related proteins · sequence + function similarity

Co-cited proteins · studied together in the literature

Transcription antitermination protein NusB 6 shared papers
Putative general secretion pathway protein B 1 shared papers
Large ribosomal subunit protein uL3 2 shared papers
Small ribosomal subunit protein uS17 2 shared papers
Small ribosomal subunit protein uS19 2 shared papers
Small ribosomal subunit protein uS3 2 shared papers
Transcription termination/antitermination protein NusG 1 shared papers
Small ribosomal subunit protein uS4 2 shared papers
Nus factor SuhB 1 shared papers
DNA-directed RNA polymerase subunit omega 1 shared papers
Transcription termination/antitermination protein NusA 1 shared papers
DNA-directed RNA polymerase subunit beta' 1 shared papers

Literature · 23 cited papers

Structure-Based Mechanisms of a Molecular RNA Polymerase/Chaperone Machine Required for Ribosome Biosynthesis. Mol. Cell · 2020
Structural basis of co-translational quality control by ArfA and RF2 bound to ribosome. Nature · 2017
Translational termination without a stop codon. Science · 2016
Structural basis for ArfA-RF2-mediated translation termination on mRNAs lacking stop codons. Nature · 2017
Mechanistic insights into the alternative translation termination by ArfA and RF2. Nature · 2017
A new system for naming ribosomal proteins. Curr. Opin. Struct. Biol. · 2014
Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex. Mol. Cell · 2008
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol. Syst. Biol. · 2006
Structures of the bacterial ribosome at 3.5 A resolution. Science · 2005
Assembly of an RNA-protein complex. Binding of NusB and NusE (S10) proteins to boxA RNA nucleates the formation of the antitermination complex involved in controlling rRNA transcription in Escherichia coli. J. Biol. Chem. · 2005
Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement. Cell · 2003
All-atom homology model of the Escherichia coli 30S ribosomal subunit. Nat. Struct. Biol. · 2002
… and 11 more in the literature graph

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