Large ribosomal subunit protein bL12

Function

The binding site for several of the GTPase factors involved in protein synthesis (IF-2, EF-Tu, EF-G and RF3). Is thus essential for accurate translation. Deletion of 1 of the L12 dimers from the ribosome (by deleting the binding site on L10) leads to decreased IF-2 association with the 70S ribosome and decreased stimulation of the GTPase activity of EF-G.

Classification

Family (Pfam)

PF00542 Ribosomal_L12, PF16320 Ribosomal_L12_N

InterPro

Ribosomal_bL12, Ribosomal_bL12_C, Ribosomal_bL12_C/ClpS-like, Ribosomal_bL12_oligo, Ribosomal_bL12_oligo_N_sf

Functional cluster

Ribosomal Protein L12 & Acyl Carriers

Experimental structures · PDB · 58

• 1CTF X-ray 1.70A
• 1RQS NMR
• 1RQT NMR
• 1RQU NMR
• 1RQV NMR
• 2BCW EM 11.20A
• 3J7Z EM 3.90A
• 4UY8 EM 3.80A
• 4V4V EM 15.00A
• 4V4W EM 15.00A
• 4V5M EM 7.80A
• 4V5N EM 7.60A
• … and 46 more

A predicted model is available from AlphaFold.

Gene Ontology · 10

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0022625 cytosolic large ribosomal subunit
• GO:0015934 large ribosomal subunit
• GO:0003729 mRNA binding
• GO:0042803 protein homodimerization activity
• GO:0043022 ribosome binding
• GO:0003735 structural constituent of ribosome
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Drugs targeting this protein · 62

• TELITHROMYCIN inhibitor
• DEMECLOCYCLINE HYDROCHLORIDE inhibitor
• ERYTHROMYCIN LACTOBIONATE inhibitor
• ERYTHROMYCIN STEARATE inhibitor
• CLINDAMYCIN HYDROCHLORIDE inhibitor
• CLINDAMYCIN PALMITATE HYDROCHLORIDE inhibitor
• QUINUPRISTIN inhibitor
• ERYTHROMYCIN ETHYLSUCCINATE inhibitor
• DOXYCYCLINE inhibitor
• CHLORAMPHENICOL SODIUM SUCCINATE inhibitor
• MINOCYCLINE HYDROCHLORIDE inhibitor
• DALFOPRISTIN inhibitor
• TETRACYCLINE PHOSPHATE COMPLEX inhibitor
• DIRITHROMYCIN inhibitor
• TEDIZOLID inhibitor
• LINEZOLID inhibitor
• CHLORAMPHENICOL inhibitor
• TETRACYCLINE inhibitor
• KANAMYCIN SULFATE inhibitor
• LINCOMYCIN inhibitor
• OXYTETRACYCLINE inhibitor
• OXYTETRACYCLINE HYDROCHLORIDE inhibitor
• DELPAZOLID inhibitor
• CLARITHROMYCIN inhibitor
• BEKANAMYCIN inhibitor
• ERAVACYCLINE inhibitor
• OMADACYCLINE TOSYLATE inhibitor
• TYLVALOSIN TARTRATE inhibitor
• TEDIZOLID PHOSPHATE inhibitor
• GAMITHROMYCIN inhibitor
• CHLORTETRACYCLINE HYDROCHLORIDE inhibitor
• METHACYCLINE HYDROCHLORIDE inhibitor
• PAROMOMYCIN SULFATE inhibitor
• SISOMICIN inhibitor
• ERYTHROMYCIN ESTOLATE inhibitor
• ERAVACYCLINE DIHYDROCHLORIDE inhibitor
• JOSAMYCIN inhibitor
• PLAZOMICIN SULFATE inhibitor
• MECLOCYCLINE SULFOSALICYLATE inhibitor
• TILDIPIROSIN inhibitor
• QUINUPRISTIN/DALFOPRISTIN inhibitor
• CLINDAMYCIN PHOSPHATE inhibitor
• STREPTOMYCIN SULFATE inhibitor
• DIHYDROSTREPTOMYCIN SULFATE inhibitor
• CONTEZOLID inhibitor
• ERYTHROMYCIN GLUCEPTATE inhibitor
• LEFAMULIN ACETATE inhibitor
• CADAZOLID inhibitor
• TIGECYCLINE inhibitor
• OXYTETRACYCLINE CALCIUM inhibitor
• SPECTINOMYCIN HYDROCHLORIDE inhibitor
• DOXYCYCLINE HYCLATE inhibitor
• NETILMICIN SULFATE inhibitor
• PIRLIMYCIN HYDROCHLORIDE inhibitor
• CETHROMYCIN inhibitor
• CONTEZOLID ACEFOSAMIL inhibitor
• AMIKACIN SULFATE inhibitor
• NAFITHROMYCIN inhibitor
• TETRACYCLINE HYDROCHLORIDE inhibitor
• RADEZOLID inhibitor
• AZITHROMYCIN inhibitor
• ERYTHROMYCIN inhibitor

Related proteins · sequence + function similarity

• Large ribosomal subunit protein bL12 1.00
• Large ribosomal subunit protein bL12 1.00
• Large ribosomal subunit protein bL12 1.00
• Large ribosomal subunit protein bL12 1.00
• Large ribosomal subunit protein bL12 1.00
• Large ribosomal subunit protein bL12 1.00
• Large ribosomal subunit protein bL12 1.00
• Large ribosomal subunit protein bL12 1.00
• Large ribosomal subunit protein bL12 1.00
• Large ribosomal subunit protein bL12 1.00
• Large ribosomal subunit protein bL12 1.00
• Large ribosomal subunit protein bL12 1.00

Co-cited proteins · studied together in the literature

• Large ribosomal subunit protein uL10 5 shared papers
• Large ribosomal subunit protein uL10 2 shared papers
• Large ribosomal subunit protein bL12 2 shared papers
• Large ribosomal subunit protein uL11 3 shared papers
• Large ribosomal subunit protein uL10 1 shared papers
• Large ribosomal subunit protein uL10 1 shared papers
• Large ribosomal subunit protein bL12 1 shared papers
• Large ribosomal subunit protein uL1 1 shared papers
• Large ribosomal subunit protein uL10 1 shared papers
• DNA-directed RNA polymerase subunit beta 1 shared papers
• Large ribosomal subunit protein bL12 1 shared papers
• Inhibitor of vertebrate lysozyme 1 shared papers

Literature · 19 cited papers

• Molecular basis for the ribosome functioning as an L-tryptophan sensor. Cell Rep. · 2014
• A new system for naming ribosomal proteins. Curr. Opin. Struct. Biol. · 2014
• Bacterial ribosome requires multiple L12 dimers for efficient initiation and elongation of protein synthesis involving IF2 and EF-G. Nucleic Acids Res. · 2012
• Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol. Syst. Biol. · 2006
• Structural basis for the function of the ribosomal L7/12 stalk in factor binding and GTPase activation. Cell · 2005
• Heptameric (L12)6/L10 rather than canonical pentameric complexes are found by tandem MS of intact ribosomes from thermophilic bacteria. Proc. Natl. Acad. Sci. U.S.A. · 2005
• Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry. Anal. Biochem. · 1999
• Small genes/gene-products in Escherichia coli K-12. FEMS Microbiol. Lett. · 1998
• Protein identification with N and C-terminal sequence tags in proteome projects. J. Mol. Biol. · 1998
• Conformational independence of N- and C-domains in ribosomal protein L7/L12 and in the complex with protein L10. FEBS Lett. · 1998
• Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis · 1997
• Escherichia coli proteome analysis using the gene-protein database. Electrophoresis · 1997
• … and 7 more in the literature graph