DNA-directed RNA polymerase subunit alpha
Also known as: JW3257, b3295, pez, phs, rpoA, sez
Function
DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.
Classification
- Family (Pfam)
- PF01000 RNA_pol_A_bac, PF03118 RNA_pol_A_CTD, PF01193 RNA_pol_L
- InterPro
- DNA-dir_RNA_pol_insert, DNA-dir_RNA_pol_RpoA/D/Rpb3, DNA-dir_RpoA, RBP11-like, RNAP_asu_C, RNApol_insert_sf
- Functional cluster
- AAA+ ATPases & Chaperones
Experimental structures · PDB · 370
- 1BDF X-ray 2.50A
- 1COO NMR
- 1LB2 X-ray 3.10A
- 1XS9 NMR
- 3IYD EM
- 3K4G X-ray 2.05A
- 3LU0 EM
- 3N4M X-ray 2.99A
- 3N97 X-ray 3.25A
- 4JK1 X-ray 3.90A
- 4JK2 X-ray 4.20A
- 4KMU X-ray 3.85A
- … and 358 more
A predicted model is available from AlphaFold.
Gene Ontology · 19
- GO:0005737 cytoplasm
- GO:0005829 cytosol
- GO:0000428 DNA-directed RNA polymerase complex
- GO:0016020 membrane
- GO:0003677 DNA binding
- GO:0003899 DNA-directed RNA polymerase activity
- GO:0046983 protein dimerization activity
- GO:0044780 bacterial-type flagellum assembly
- GO:0071973 bacterial-type flagellum-dependent cell motility
- GO:0048870 cell motility
- GO:0036460 cellular response to cell envelope stress
- GO:0006352 DNA-templated transcription initiation
- GO:0006879 intracellular iron ion homeostasis
- GO:0042128 nitrate assimilation
- GO:0032784 regulation of DNA-templated transcription elongation
- GO:2000142 regulation of DNA-templated transcription initiation
- GO:0009408 response to heat
- GO:0090605 submerged biofilm formation
- GO:0031564 transcription antitermination
Drugs targeting this protein · 7
- RIFAXIMIN inhibitor
- RIFAPENTINE inhibitor
- RIFAMYCIN SODIUM inhibitor
- RIFALAZIL inhibitor
- RIFAMPIN inhibitor
- RIFABUTIN inhibitor
- RIFAQUIZINONE inhibitor
Related proteins · sequence + function similarity
- DNA-directed RNA polymerase subunit alpha 1.00
- DNA-directed RNA polymerase subunit alpha 1.00
- DNA-directed RNA polymerase subunit alpha 1.00
- DNA-directed RNA polymerase subunit alpha 1.00
- DNA-directed RNA polymerase subunit alpha 1.00
- DNA-directed RNA polymerase subunit alpha 1.00
- DNA-directed RNA polymerase subunit alpha 1.00
- DNA-directed RNA polymerase subunit alpha 1.00
- DNA-directed RNA polymerase subunit alpha 1.00
- DNA-directed RNA polymerase subunit alpha 1.00
- DNA-directed RNA polymerase subunit alpha 1.00
- DNA-directed RNA polymerase subunit alpha 1.00
Co-cited proteins · studied together in the literature
- DNA-binding transcriptional dual regulator CRP 4 shared papers
- DNA-directed RNA polymerase subunit beta' 6 shared papers
- NAD--protein ADP-ribosyltransferase modA 2 shared papers
- Small ribosomal subunit protein uS4 3 shared papers
- DNA-directed RNA polymerase subunit beta 6 shared papers
- NAD--protein ADP-ribosyltransferase modB 2 shared papers
- Large ribosomal subunit protein bL17 2 shared papers
- RNA polymerase sigma factor RpoD 3 shared papers
- DNA-directed RNA polymerase subunit omega 3 shared papers
- Methionyl-tRNA formyltransferase 1 shared papers
- Peptidyl-lysine N-acetyltransferase PatZ 1 shared papers
- Small ribosomal subunit protein uS11 1 shared papers
Literature · 23 cited papers
- Structure-Based Mechanisms of a Molecular RNA Polymerase/Chaperone Machine Required for Ribosome Biosynthesis. Mol. Cell · 2020
- Transcription inhibition by the depsipeptide antibiotic salinamide A. Elife · 2014
- Involvement of protein acetylation in glucose-induced transcription of a stress-responsive promoter. Mol. Microbiol. · 2011
- Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. Proc. Natl. Acad. Sci. U.S.A. · 2009
- Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol. Syst. Biol. · 2006
- ModA and ModB, two ADP-ribosyltransferases encoded by bacteriophage T4: catalytic properties and mutation analysis. J. Bacteriol. · 2004
- Structural basis of transcription activation: the CAP-alpha CTD-DNA complex. Science · 2002
- Overexpression, purification, and partial characterization of ADP-ribosyltransferases modA and modB of bacteriophage T4. Gene Expr. · 1999
- Structure of the Escherichia coli RNA polymerase alpha subunit amino-terminal domain. Science · 1998
- Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis · 1997
- Escherichia coli proteome analysis using the gene-protein database. Electrophoresis · 1997
- The complete genome sequence of Escherichia coli K-12. Science · 1997
- … and 11 more in the literature graph
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