Acyl carrier protein, mitochondrial
Also known as: NDUFAB1
Function
Carrier of the growing fatty acid chain in fatty acid biosynthesis (By similarity). Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. Accessory protein, of the core iron-sulfur cluster (ISC) assembly complex, that regulates, in association with LYRM4, the stability and the cysteine desulfurase activity of NFS1 and participates in the [2Fe-2S] clusters assembly on the scaffolding protein ISCU. The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (By similarity).
Classification
- Family (Pfam)
- PF00550 PP-binding
- InterPro
- ACP, ACP-like_sf, PP-bd_ACP, Ppantetheine_attach_site
- Functional cluster
- Chloroplast & RuBisCO Photosynthetic Proteins
Experimental structures · PDB · 41
- 2DNW NMR
- 5OOL EM 3.06A
- 5OOM EM 3.03A
- 5XTB EM 3.40A
- 5XTC EM 3.70A
- 5XTD EM 3.70A
- 5XTH EM 3.90A
- 5XTI EM 17.40A
- 6ODD X-ray 2.00A
- 7A5H EM 3.30A
- 7A5J EM 3.10A
- 7O9K EM 3.10A
- … and 29 more
A predicted model is available from AlphaFold.
Gene Ontology · 20
- GO:0099128 mitochondrial [2Fe-2S] assembly complex
- GO:0005743 mitochondrial inner membrane
- GO:0005759 mitochondrial matrix
- GO:0031966 mitochondrial membrane
- GO:0005739 mitochondrion
- GO:0045271 respiratory chain complex I
- GO:0000035 acyl binding
- GO:0000036 acyl carrier activity
- GO:0005509 calcium ion binding
- GO:0005504 fatty acid binding
- GO:0140978 mitochondrial large ribosomal subunit binding
- GO:0005198 structural molecule activity
- GO:0044571 [2Fe-2S] cluster assembly
- GO:0009060 aerobic respiration
- GO:0006633 fatty acid biosynthetic process
- GO:0016226 iron-sulfur cluster assembly
- GO:0006120 mitochondrial electron transport, NADH to ubiquinone
- GO:1902775 mitochondrial large ribosomal subunit assembly
- GO:0009249 protein lipoylation
- GO:0042776 proton motive force-driven mitochondrial ATP synthesis
Drugs targeting this protein · 2
- METFORMIN HYDROCHLORIDE inhibitor
- ME-344 inhibitor
Related proteins · sequence + function similarity
- Acyl carrier protein, mitochondrial 1.00
- Acyl carrier protein, mitochondrial 1.00
- Acyl carrier protein, mitochondrial 0.95
- Acyl carrier protein, mitochondrial 0.95
- Acyl carrier protein, mitochondrial 0.88
- Acyl carrier protein 2, mitochondrial 0.77
- Acyl carrier protein 1, mitochondrial 0.77
- Acyl carrier protein 3, mitochondrial 0.75
- Acyl carrier protein, mitochondrial 0.74
- Putative acyl carrier protein, mitochondrial 0.74
- Acyl carrier protein, mitochondrial 0.73
- Acyl carrier protein, mitochondrial 0.72
Co-cited proteins · studied together in the literature
- Large ribosomal subunit protein uL4m 1 shared papers
- Protein AMBP 1 shared papers
- LYR motif-containing protein 4 1 shared papers
- Mitochondrial assembly of ribosomal large subunit protein 1 1 shared papers
- Cysteine desulfurase 1 shared papers
- Small ribosomal subunit protein mS27 1 shared papers
- NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial 3 shared papers
- Mitochondrial ribosome and complex I assembly factor AltMIEF1 1 shared papers
- NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 2 shared papers
- NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial 2 shared papers
- NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 2 shared papers
- NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 2 shared papers
Literature · 16 cited papers
- A late-stage assembly checkpoint of the human mitochondrial ribosome large subunit. Nat. Commun. · 2022
- Structure of the Human ACP-ISD11 Heterodimer. Biochemistry · 2019
- MIEF1 microprotein regulates mitochondrial translation. Biochemistry · 2018
- Structures of the human mitochondrial ribosome in native states of assembly. Nat. Struct. Mol. Biol. · 2017
- Accessory subunits are integral for assembly and function of human mitochondrial complex I. Nature · 2016
- Mitochondrial protein interaction mapping identifies regulators of respiratory chain function. Mol. Cell · 2016
- N-terminome analysis of the human mitochondrial proteome. Proteomics · 2015
- An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
- The radical-binding lipocalin A1M binds to a Complex I subunit and protects mitochondrial structure and function. Antioxid. Redox Signal. · 2013
- Initial characterization of the human central proteome. BMC Syst. Biol. · 2011
- Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini. Proc. Natl. Acad. Sci. U.S.A. · 2009
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. · 2004
- … and 4 more in the literature graph