Large ribosomal subunit protein uL5

Function

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53. Promotes nucleolar location of PML (By similarity).

Classification

Family (Pfam)

PF00281 Ribosomal_L5, PF00673 Ribosomal_L5_C

InterPro

Ribosomal_uL5, Ribosomal_uL5_C, Ribosomal_uL5_CS, Ribosomal_uL5_dom_sf, Ribosomal_uL5_euk_arc, Ribosomal_uL5_N

Functional cluster

Ribosomal S8 & Metabolic Enzymes

Experimental structures · PDB · 146

• 4UG0 EM
• 4V6X EM 5.00A
• 4XXB X-ray 2.40A
• 5AJ0 EM 3.50A
• 5LKS EM 3.60A
• 5T2C EM 3.60A
• 6IP5 EM 3.90A
• 6IP6 EM 4.50A
• 6IP8 EM 3.90A
• 6LQM EM 3.09A
• 6LSR EM 3.13A
• 6LSS EM 3.23A
• … and 134 more

A predicted model is available from AlphaFold.

Gene Ontology · 28

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0070062 extracellular exosome
• GO:0016020 membrane
• GO:0005730 nucleolus
• GO:0005654 nucleoplasm
• GO:0032991 protein-containing complex
• GO:0008097 5S rRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:1990948 ubiquitin ligase inhibitor activity
• GO:0031625 ubiquitin protein ligase binding
• GO:0002181 cytoplasmic translation
• GO:1901740 negative regulation of myoblast fusion
• GO:0032435 negative regulation of proteasomal ubiquitin-dependent protein catabolic process
• GO:2000059 negative regulation of ubiquitin-dependent protein catabolic process
• GO:1901798 positive regulation of signal transduction by p53 class mediator
• GO:0034504 protein localization to nucleus
• GO:0006605 protein targeting
• GO:1901796 regulation of signal transduction by p53 class mediator
• GO:0000027 ribosomal large subunit assembly
• GO:0042273 ribosomal large subunit biogenesis
• GO:0006364 rRNA processing
• GO:0007283 spermatogenesis
• GO:0006941 striated muscle contraction
• GO:0006412 translation

Disease associations

• Diamond-Blackfan anemia 7 MONDO:0012938

Drugs targeting this protein · 2

• CYCLOHEXIMIDE inhibitor
• ATALUREN modulator

Related proteins · sequence + function similarity

• Large ribosomal subunit protein uL5 1.00
• Large ribosomal subunit protein uL5 1.00
• Large ribosomal subunit protein uL5 1.00
• Large ribosomal subunit protein uL5 1.00
• Large ribosomal subunit protein uL5 1.00
• Large ribosomal subunit protein uL5 1.00
• Large ribosomal subunit protein uL5 0.98
• Large ribosomal subunit protein uL5 0.91
• Large ribosomal subunit protein uL5B 0.89
• Large ribosomal subunit protein uL5B 0.89
• Large ribosomal subunit protein uL5y/uL5x/uL5w 0.88
• Large ribosomal subunit protein uL5 0.88

Co-cited proteins · studied together in the literature

• Ribosome biogenesis protein NOP53 1 shared papers
• Large ribosomal subunit protein uL18 5 shared papers
• Ribosome biogenesis protein NOP53 3 shared papers
• Small ribosomal subunit protein eS17 1 shared papers
• Small ribosomal subunit protein eS7 1 shared papers
• Ribosome biogenesis regulatory protein homolog 2 shared papers
• p21-activated protein kinase-interacting protein 1 1 shared papers
• Transcription factor IIIA 1 shared papers
• Ribosome production factor 2 homolog 2 shared papers
• HEAT repeat-containing protein 3 1 shared papers
• Large ribosomal subunit protein eL29 1 shared papers
• Large ribosomal subunit protein uL22 2 shared papers

Literature · 24 cited papers

• Structure of nascent 5S RNPs at the crossroad between ribosome assembly and MDM2-p53 pathways. Nat. Struct. Mol. Biol. · 2023
• Structural snapshots of human pre-60S ribosomal particles before and after nuclear export. Nat. Commun. · 2020
• Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation. Nat. Struct. Mol. Biol. · 2017
• PICT-1 is a key nucleolar sensor in DNA damage response signaling that regulates apoptosis through the RPL11-MDM2-p53 pathway. Oncotarget · 2016
• N-terminome analysis of the human mitochondrial proteome. Proteomics · 2015
• GLTSCR2/PICT1 links mitochondrial stress and Myc signaling. Proc. Natl. Acad. Sci. U.S.A. · 2014
• A new system for naming ribosomal proteins. Curr. Opin. Struct. Biol. · 2014
• An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
• The 5S RNP couples p53 homeostasis to ribosome biogenesis and nucleolar stress. Cell Rep. · 2013
• Structures of the human and Drosophila 80S ribosome. Nature · 2013
• Toward a comprehensive characterization of a human cancer cell phosphoproteome. J. Proteome Res. · 2013
• N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc. Natl. Acad. Sci. U.S.A. · 2012
• … and 12 more in the literature graph