Proteasome subunit beta type-5
Also known as: LMPX, MB1, PSMB5, X
Function
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB5 displays a chymotrypsin-like activity.
Classification
- Family (Pfam)
- PF00227 Proteasome
- InterPro
- Ntn_hydrolases_N, Pept_T1A_subB, Proteasome_bsu_CS, Proteasome_sua/b, Proteasome_suB-type
- Functional cluster
- Phosphoglucosamine/Phosphomannomutases
Experimental structures · PDB · 113
- 4R3O X-ray 2.60A
- 4R67 X-ray 2.89A
- 5A0Q EM 3.50A
- 5GJQ EM 4.50A
- 5GJR EM 3.50A
- 5L4G EM 4.02A
- 5L5W X-ray 2.80A
- 5L5X X-ray 2.90A
- 5L5Y X-ray 2.70A
- 5L5Z X-ray 2.70A
- 5L60 X-ray 2.70A
- 5L61 X-ray 2.80A
- … and 101 more
A predicted model is available from AlphaFold.
Gene Ontology · 28
- GO:0005737 cytoplasm
- GO:0005829 cytosol
- GO:0070062 extracellular exosome
- GO:0005654 nucleoplasm
- GO:0005634 nucleus
- GO:0000502 proteasome complex
- GO:0005839 proteasome core complex
- GO:0019774 proteasome core complex, beta-subunit complex
- GO:0034515 proteasome storage granule
- GO:0008021 synaptic vesicle
- GO:0004175 endopeptidase activity
- GO:0008233 peptidase activity
- GO:0004298 threonine-type endopeptidase activity
- GO:0006915 apoptotic process
- GO:0071357 cellular response to type I interferon
- GO:0006974 DNA damage response
- GO:0006281 DNA repair
- GO:0030317 flagellated sperm motility
- GO:0051321 meiotic cell cycle
- GO:0010498 proteasomal protein catabolic process
- GO:0010499 proteasomal ubiquitin-independent protein catabolic process
- GO:0043248 proteasome assembly
- GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
- GO:0006508 proteolysis
- GO:2000045 regulation of G1/S transition of mitotic cell cycle
- GO:0061136 regulation of proteasomal protein catabolic process
- GO:0006979 response to oxidative stress
- GO:0007283 spermatogenesis
Drugs targeting this protein · 7
- OPROZOMIB inhibitor
- IXAZOMIB inhibitor
- BORTEZOMIB inhibitor
- IXAZOMIB CITRATE inhibitor
- MARIZOMIB inhibitor
- CARFILZOMIB inhibitor
- BORTEZOMIB D-MANNITOL inhibitor
Related proteins · sequence + function similarity
- Proteasome subunit beta type-5 0.98
- Proteasome subunit beta type-5 0.97
- Proteasome subunit beta type-5 0.95
- Proteasome subunit beta type-5 0.91
- Proteasome subunit beta type-8 0.91
- Proteasome subunit beta type-5 0.90
- Proteasome subunit beta type-8 0.90
- Proteasome subunit beta type-8 0.90
- Proteasome subunit beta type-8 0.89
- Proteasome subunit beta type-5-B 0.89
- Proteasome subunit beta type-5-A 0.89
- Proteasome subunit beta type-5 0.87
Co-cited proteins · studied together in the literature
- Proteasome subunit beta type-6 15 shared papers
- Proteasome subunit alpha type-1 13 shared papers
- Proteasome subunit beta type-8 5 shared papers
- Proteasome subunit beta type-1 13 shared papers
- Proteasome subunit beta type-4 14 shared papers
- Proteasome subunit beta type-3 13 shared papers
- Proteasome subunit beta type-2 13 shared papers
- Proteasome subunit alpha type-6 12 shared papers
- Proteasome subunit beta type-7 12 shared papers
- Proteasome subunit alpha type-4 12 shared papers
- Proteasome subunit alpha type-2 12 shared papers
- Proteasome maturation protein 2 shared papers
Literature · 34 cited papers
- AKIRIN2 controls the nuclear import of proteasomes in vertebrates. Nature · 2021
- The inhibition mechanism of human 20S proteasomes enables next-generation inhibitor design. Science · 2016
- An atomic structure of the human 26S proteasome. Nat. Struct. Mol. Biol. · 2016
- Structure of the human 26S proteasome at a resolution of 3.9 Aa. Proc. Natl. Acad. Sci. U.S.A. · 2016
- Human 20S proteasome activity towards fluorogenic peptides of various chain lengths. Biol. Chem. · 2016
- Cryo-EM reveals the conformation of a substrate analogue in the human 20S proteasome core. Nat. Commun. · 2015
- Crystal structure of the human 20S proteasome in complex with carfilzomib. Structure · 2015
- An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
- Molecular architecture and assembly of the eukaryotic proteasome. Annu. Rev. Biochem. · 2013
- Initial characterization of the human central proteome. BMC Syst. Biol. · 2011
- Different mutants of PSMB5 confer varying bortezomib resistance in T lymphoblastic lymphoma/leukemia cells derived from the Jurkat cell line. Exp. Hematol. · 2009
- Regulation of estrogen receptor alpha expression in human breast cancer cells by sulforaphane. J. Nutr. Biochem. · 2009
- … and 22 more in the literature graph
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