Small ribosomal subunit protein bS1

UniProt P0AG67 Organism Escherichia coli (strain K12) Gene JW0894, b0911, rpsA, ssyF

Also known as: JW0894, b0911, rpsA, ssyF

Function

Required for translation of most natural mRNAs except for leaderless mRNA. Binds mRNA upstream of the Shine-Dalgarno (SD) sequence and helps it bind to the 30S ribosomal subunit; acts as an RNA chaperone to unfold structured mRNA on the ribosome but is not essential for mRNAs with strong SDs and little 5'-UTR structure, thus it may help fine-tune which mRNAs that are translated. Unwinds dsRNA by binding to transiently formed ssRNA regions; binds about 10 nucleotides. Has a preference for polypyrimidine tracts. Negatively autoregulates its own translation.

Classification

Family (Pfam): PF00575 S1
InterPro: NA-bd_OB-fold, Ribos_protein_bS1-like, Ribosomal_bS1, Ribosomal_protein_S1-like, S1_domain
Functional cluster: Co-Chaperonins & Elongation Factors

Experimental structures · PDB · 69

2BH8 X-ray 1.90A
2KHI NMR
2KHJ NMR
4Q7J X-ray 2.90A
4R71 X-ray 3.21A
5XQ5 NMR
6BU8 EM 3.50A
6H4N EM 3.00A
6H58 EM 7.90A
6VU3 EM 3.70A
6VYQ EM 3.70A
6VYR EM 3.80A
… and 57 more

A predicted model is available from AlphaFold.

Gene Ontology · 12

Drugs targeting this protein · 62

TELITHROMYCIN inhibitor
DEMECLOCYCLINE HYDROCHLORIDE inhibitor
ERYTHROMYCIN LACTOBIONATE inhibitor
ERYTHROMYCIN STEARATE inhibitor
CLINDAMYCIN HYDROCHLORIDE inhibitor
CLINDAMYCIN PALMITATE HYDROCHLORIDE inhibitor
QUINUPRISTIN inhibitor
ERYTHROMYCIN ETHYLSUCCINATE inhibitor
DOXYCYCLINE inhibitor
CHLORAMPHENICOL SODIUM SUCCINATE inhibitor
MINOCYCLINE HYDROCHLORIDE inhibitor
DALFOPRISTIN inhibitor
TETRACYCLINE PHOSPHATE COMPLEX inhibitor
DIRITHROMYCIN inhibitor
TEDIZOLID inhibitor
LINEZOLID inhibitor
CHLORAMPHENICOL inhibitor
TETRACYCLINE inhibitor
KANAMYCIN SULFATE inhibitor
LINCOMYCIN inhibitor
OXYTETRACYCLINE inhibitor
OXYTETRACYCLINE HYDROCHLORIDE inhibitor
DELPAZOLID inhibitor
CLARITHROMYCIN inhibitor
BEKANAMYCIN inhibitor
ERAVACYCLINE inhibitor
OMADACYCLINE TOSYLATE inhibitor
TYLVALOSIN TARTRATE inhibitor
TEDIZOLID PHOSPHATE inhibitor
GAMITHROMYCIN inhibitor
CHLORTETRACYCLINE HYDROCHLORIDE inhibitor
METHACYCLINE HYDROCHLORIDE inhibitor
PAROMOMYCIN SULFATE inhibitor
SISOMICIN inhibitor
ERYTHROMYCIN ESTOLATE inhibitor
ERAVACYCLINE DIHYDROCHLORIDE inhibitor
JOSAMYCIN inhibitor
PLAZOMICIN SULFATE inhibitor
MECLOCYCLINE SULFOSALICYLATE inhibitor
TILDIPIROSIN inhibitor
QUINUPRISTIN/DALFOPRISTIN inhibitor
CLINDAMYCIN PHOSPHATE inhibitor
STREPTOMYCIN SULFATE inhibitor
DIHYDROSTREPTOMYCIN SULFATE inhibitor
CONTEZOLID inhibitor
ERYTHROMYCIN GLUCEPTATE inhibitor
LEFAMULIN ACETATE inhibitor
CADAZOLID inhibitor
TIGECYCLINE inhibitor
OXYTETRACYCLINE CALCIUM inhibitor
SPECTINOMYCIN HYDROCHLORIDE inhibitor
DOXYCYCLINE HYCLATE inhibitor
NETILMICIN SULFATE inhibitor
PIRLIMYCIN HYDROCHLORIDE inhibitor
CETHROMYCIN inhibitor
CONTEZOLID ACEFOSAMIL inhibitor
AMIKACIN SULFATE inhibitor
NAFITHROMYCIN inhibitor
TETRACYCLINE HYDROCHLORIDE inhibitor
RADEZOLID inhibitor
AZITHROMYCIN inhibitor
ERYTHROMYCIN inhibitor

Related proteins · sequence + function similarity

Co-cited proteins · studied together in the literature

Cytidylate kinase 2 shared papers
Endoribonuclease RegB 1 shared papers
Small ribosomal subunit protein uS2 3 shared papers
Small ribosomal subunit protein uS5 2 shared papers
RNA-directed RNA polymerase subunit beta 3 shared papers
Small ribosomal subunit protein uS15 1 shared papers
NAD--protein ADP-ribosyltransferase modB 1 shared papers
Integration host factor subunit beta 1 shared papers
Nucleoside diphosphate kinase 1 shared papers
Small ribosomal subunit protein uS3 2 shared papers
Elongation factor Ts 2 shared papers
Large ribosomal subunit assembly factor BipA 1 shared papers

Literature · 36 cited papers

A viral ADP-ribosyltransferase attaches RNA chains to host proteins. Nature · 2023
Ribosome collisions induce mRNA cleavage and ribosome rescue in bacteria. Nature · 2022
Molecular insights into replication initiation by Qbeta replicase using ribosomal protein S1. Nucleic Acids Res. · 2014
A new system for naming ribosomal proteins. Curr. Opin. Struct. Biol. · 2014
Escherichia coli ribosomal protein S1 unfolds structured mRNAs onto the ribosome for active translation initiation. PLoS Biol. · 2013
Ribosomal protein S1 functions as a termination factor in RNA synthesis by Qbeta phage replicase. Nat. Commun. · 2013
Ribosomal protein S1 unwinds double-stranded RNA in multiple steps. Proc. Natl. Acad. Sci. U.S.A. · 2012
Multiple activities of RNA-binding proteins S1 and Hfq. Biochimie · 2012
Probing the relationship between Gram-negative and Gram-positive S1 proteins by sequence analysis. Nucleic Acids Res. · 2009
Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli. Mol. Cell. Proteomics · 2009
Ribosomal protein S1 influences trans-translation in vitro and in vivo. Nucleic Acids Res. · 2007
Ribosomal protein S1 is not essential for the trans-translation machinery. J. Mol. Biol. · 2007
… and 24 more in the literature graph

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