Echinoderm microtubule-associated protein-like 4

Function

Essential for the formation and stability of microtubules (MTs). Required for the organization of the mitotic spindle and for the proper attachment of kinetochores to MTs. Promotes the recruitment of NUDC to the mitotic spindle for mitotic progression.

Classification

Family (Pfam)

PF23409 Beta-prop_EML, PF23414 Beta-prop_EML_2, PF03451 HELP

InterPro

Beta-prop_EML-like_2nd, Beta-prop_EML_1st, HELP, Quinoprotein_ADH-like_sf, WD40/YVTN_repeat-like_dom_sf, WD40_repeat_dom_sf, WD40_rpt, WD_repeat_EMAP

Functional cluster

Protein Serine/Threonine Kinases

Experimental structures · PDB · 1

• 4CGC X-ray 2.90A

A predicted model is available from AlphaFold.

Gene Ontology · 18

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0045171 intercellular bridge
• GO:0016020 membrane
• GO:0005874 microtubule
• GO:0015630 microtubule cytoskeleton
• GO:0005815 microtubule organizing center
• GO:0030496 midbody
• GO:0072686 mitotic spindle
• GO:0043014 alpha-tubulin binding
• GO:0048487 beta-tubulin binding
• GO:0008017 microtubule binding
• GO:0008608 attachment of spindle microtubules to kinetochore
• GO:0051301 cell division
• GO:0000226 microtubule cytoskeleton organization
• GO:0007017 microtubule-based process
• GO:0000278 mitotic cell cycle
• GO:0007080 mitotic metaphase chromosome alignment

Drugs targeting this protein · 4

• CERITINIB inhibitor
• LORLATINIB inhibitor
• ALECTINIB HYDROCHLORIDE inhibitor
• CRIZOTINIB inhibitor

Related proteins · sequence + function similarity

• Echinoderm microtubule-associated protein-like 4 0.97
• Echinoderm microtubule-associated protein-like 4 0.94
• Echinoderm microtubule-associated protein-like 4 0.93
• Echinoderm microtubule-associated protein-like 1 0.89
• Echinoderm microtubule-associated protein-like 1 0.88
• Echinoderm microtubule-associated protein-like 1 0.88
• 77 kDa echinoderm microtubule-associated protein 0.80
• 77 kDa echinoderm microtubule-associated protein 0.80
• Cilia- and flagella-associated protein 52 0.75
• Cilia- and flagella-associated protein 52 0.74
• Cilia- and flagella-associated protein 251 0.74
• Cilia- and flagella-associated protein 52 0.74

Co-cited proteins · studied together in the literature

• Nuclear migration protein nudC 1 shared papers
• ALK tyrosine kinase receptor 1 shared papers
• Echinoderm microtubule-associated protein-like 2 1 shared papers
• Echinoderm microtubule-associated protein-like 3 1 shared papers
• Serine/threonine-protein kinase Nek6 1 shared papers
• Serine/threonine-protein kinase Nek7 1 shared papers
• Echinoderm microtubule-associated protein-like 1 1 shared papers

Literature · 19 cited papers

• Mitotic phosphorylation by NEK6 and NEK7 reduces the microtubule affinity of EML4 to promote chromosome congression. Sci. Signal. · 2019
• EML4 promotes the loading of NUDC to the spindle for mitotic progression. Cell Cycle · 2015
• Microtubule association of EML proteins and the EML4-ALK variant 3 oncoprotein require an N-terminal trimerization domain. Biochem. J. · 2015
• An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
• Toward a comprehensive characterization of a human cancer cell phosphoproteome. J. Proteome Res. · 2013
• N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc. Natl. Acad. Sci. U.S.A. · 2012
• System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci. Signal. · 2011
• Initial characterization of the human central proteome. BMC Syst. Biol. · 2011
• Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci. Signal. · 2010
• Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci. Signal. · 2009
• Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal. Chem. · 2009
• A quantitative atlas of mitotic phosphorylation. Proc. Natl. Acad. Sci. U.S.A. · 2008
• … and 7 more in the literature graph