DNA polymerase alpha subunit B
Also known as: POLA2
Function
Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity). The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (By similarity). These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity).
Classification
- Family (Pfam)
- PF04042 DNA_pol_E_B, PF22062 OB_DPOA2, PF08418 Pol_alpha_B_N
- InterPro
- DNA_pol_a/d/e_bsu, DNA_pol_alpha_B_N_sf, DNA_pol_alpha_bsu, OB_DPOA2, Pol_alpha_B_N
- Functional cluster
- Zinc-Finger & Chromatin Regulatory Proteins
Experimental structures · PDB · 13
- 2KEB NMR
- 4E2I X-ray 5.00A
- 4Y97 X-ray 2.51A
- 5EXR X-ray 3.60A
- 7OPL EM 4.12A
- 7U5C EM 4.60A
- 8B9D EM 3.40A
- 8D0B EM 3.43A
- 8D0K EM 4.27A
- 8D9D EM 3.59A
- 8QJ7 EM 3.07A
- 8VY3 EM 2.98A
- … and 1 more
A predicted model is available from AlphaFold.
Gene Ontology · 6
Drugs targeting this protein · 6
- FLUDARABINE PHOSPHATE inhibitor
- GEMCITABINE HYDROCHLORIDE inhibitor
- CLOFARABINE inhibitor
- TROXACITABINE inhibitor
- ASPACYTARABINE inhibitor
- CYTARABINE inhibitor
Related proteins · sequence + function similarity
- DNA polymerase alpha subunit B 0.98
- DNA polymerase alpha subunit B 0.97
- DNA polymerase alpha subunit B 0.95
- DNA polymerase delta subunit 2 0.74
- DNA polymerase delta subunit 2 0.71
- DNA polymerase alpha subunit B 0.66
- DNA polymerase delta subunit 2 0.64
- DNA polymerase delta small subunit 0.63
- DNA polymerase delta subunit 2 0.63
- DNA polymerase delta subunit 2 0.62
- DNA polymerase delta small subunit 0.61
- DNA polymerase alpha subunit B 0.59
Co-cited proteins · studied together in the literature
- DNA primase small subunit 2 shared papers
- DNA primase large subunit 2 shared papers
- DNA polymerase alpha catalytic subunit 2 shared papers
Literature · 14 cited papers
- Mechanism of Concerted RNA-DNA Primer Synthesis by the Human Primosome. J. Biol. Chem. · 2016
- Toward a comprehensive characterization of a human cancer cell phosphoproteome. J. Proteome Res. · 2013
- System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci. Signal. · 2011
- Initial characterization of the human central proteome. BMC Syst. Biol. · 2011
- Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci. Signal. · 2010
- Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci. Signal. · 2009
- Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal. Chem. · 2009
- A quantitative atlas of mitotic phosphorylation. Proc. Natl. Acad. Sci. U.S.A. · 2008
- Human chromosome 11 DNA sequence and analysis including novel gene identification. Nature · 2006
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. · 2004
- Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat. Genet. · 2004
- Primase activity of human DNA polymerase alpha-primase. Divalent cations stabilize the enzyme activity of the p48 subunit. J. Biol. Chem. · 1998
- … and 2 more in the literature graph