lmmol · Proteins

DNA primase large subunit

UniProt P49643 Organism Homo sapiens Gene PRIM2, PRIM2A

Also known as: PRIM2, PRIM2A

Function

Regulatory subunit of the DNA primase complex and component of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which play an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1 (By similarity). The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity). In the primase complex, both subunits are necessary for the initial di-nucleotide formation, but the extension of the primer depends only on the catalytic subunit. Binds RNA:DNA duplex and coordinates the catalytic activities of PRIM1 and POLA2 during primase-to-polymerase switch.

Classification

Family (Pfam)
PF04104 DNA_primase_lrg, PF26466 DNA_primase_lrg_N
InterPro
DNA_primase_C, DNA_primase_lsu_euk, DNA_primase_lsu_euk/arc
Functional cluster
Aminoacyl-tRNA Synthetases

Experimental structures · PDB · 22

A predicted model is available from AlphaFold.

Gene Ontology · 8

Drugs targeting this protein · 6

Related proteins · sequence + function similarity

Co-cited proteins · studied together in the literature

Literature · 14 cited papers

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