26S proteasome complex subunit SEM1
Also known as: C7orf76, DSS1, SEM1, SHFDG1, SHFM1
Function
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. Component of the TREX-2 complex (transcription and export complex 2), composed of at least ENY2, GANP, PCID2, SEM1, and either centrin CETN2 or CETN3. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. Binds and stabilizes BRCA2 and is thus involved in the control of R-loop-associated DNA damage and thus transcription-associated genomic instability. R-loop accumulation increases in SEM1-depleted cells.
Classification
- Family (Pfam)
- PF05160 DSS1_SEM1
- InterPro
- DSS1_SEM1
- Functional cluster
- Ribosomal Protein L12 & Acyl Carriers
Experimental structures · PDB · 83
- 1IYJ X-ray 3.40A
- 1MIU X-ray 3.10A
- 1MJE X-ray 3.50A
- 3T5X X-ray 2.12A
- 5GJQ EM 4.50A
- 5GJR EM 3.50A
- 5L4K EM 4.50A
- 5LN3 EM 6.80A
- 5M32 EM 3.80A
- 5T0C EM 3.80A
- 5T0G EM 4.40A
- 5T0H EM 6.80A
- … and 71 more
A predicted model is available from AlphaFold.
Gene Ontology · 16
- GO:0005829 cytosol
- GO:0032039 integrator complex
- GO:0005654 nucleoplasm
- GO:0000502 proteasome complex
- GO:0008541 proteasome regulatory particle, lid subcomplex
- GO:0032991 protein-containing complex
- GO:0008021 synaptic vesicle
- GO:0043130 ubiquitin binding
- GO:0071357 cellular response to type I interferon
- GO:0006406 mRNA export from nucleus
- GO:0045944 positive regulation of transcription by RNA polymerase II
- GO:0010498 proteasomal protein catabolic process
- GO:0043248 proteasome assembly
- GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
- GO:0061136 regulation of proteasomal protein catabolic process
- GO:0006979 response to oxidative stress
Drugs targeting this protein · 6
- OPROZOMIB inhibitor
- IXAZOMIB inhibitor
- BORTEZOMIB inhibitor
- IXAZOMIB CITRATE inhibitor
- CARFILZOMIB inhibitor
- BORTEZOMIB D-MANNITOL inhibitor
Related proteins · sequence + function similarity
- 26S proteasome complex subunit SEM1 1.00
- 26S proteasome complex subunit SEM1 1.00
- Probable 26S proteasome complex subunit sem1 0.91
- Protein DELETION OF SUV3 SUPPRESSOR 1(I) 0.91
- 26S proteasome complex subunit rpn15 0.89
- Protein DSS1 HOMOLOG ON CHROMOSOME V 0.89
- Probable 26S proteasome complex subunit SEM1 0.83
- Putative 26S proteasome complex subunit sem-1 0.82
- Probable 26S proteasome complex subunit dss-1 0.81
- Probable 26S proteasome complex subunit dss-1 0.80
- Probable 26S proteasome complex subunit sem1 0.77
- 26S proteasome complex subunit SEM1 0.76
Co-cited proteins · studied together in the literature
- 26S proteasome complex subunit SEM1 1 shared papers
- 26S proteasome complex subunit SEM1 1 shared papers
- PCI domain-containing protein 2 2 shared papers
- Breast cancer type 2 susceptibility protein 4 shared papers
- Breast cancer type 2 susceptibility protein homolog 1 shared papers
- Breast cancer type 2 susceptibility protein homolog 1 shared papers
- Centrin-3 1 shared papers
- DNA excision repair protein ERCC-5 1 shared papers
- Transcription and mRNA export factor ENY2 1 shared papers
- Partner and localizer of BRCA2 1 shared papers
- 26S proteasome non-ATPase regulatory subunit 6 3 shared papers
- Germinal-center associated nuclear protein 1 shared papers
Literature · 15 cited papers
- An atomic structure of the human 26S proteasome. Nat. Struct. Mol. Biol. · 2016
- Structure of the human 26S proteasome at a resolution of 3.9 Aa. Proc. Natl. Acad. Sci. U.S.A. · 2016
- Non-catalytic Roles for XPG with BRCA1 and BRCA2 in Homologous Recombination and Genome Stability. Mol. Cell · 2016
- BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2. Nature · 2014
- An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
- Functional and structural characterization of the mammalian TREX-2 complex that links transcription with nuclear messenger RNA export. Nucleic Acids Res. · 2012
- A comprehensive functional characterization of BRCA2 variants associated with Fanconi anemia using mouse ES cell-based assay. Blood · 2011
- Mass spectrometric characterization of the affinity-purified human 26S proteasome complex. Biochemistry · 2007
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. · 2004
- Sem1p is a novel subunit of the 26S proteasome from Saccharomyces cerevisiae. J. Biol. Chem. · 2004
- The DNA sequence of human chromosome 7. Nature · 2003
- BRCA2 function in DNA binding and recombination from a BRCA2-DSS1-ssDNA structure. Science · 2002
- … and 3 more in the literature graph