DNA topoisomerase 4 subunit B
Also known as: JW2998, b3030, nfxD, parE
Function
Topoisomerase IV is essential for chromosome segregation; it is the principal protein responsible for decatenating newly replicated chromosomes. It relaxes supercoiled DNA. MukB stimulates the relaxation activity of topoisomerase IV and also has a modest effect on decatenation.
Classification
- Family (Pfam)
- PF00204 DNA_gyraseB, PF00986 DNA_gyraseB_C, PF02518 HATPase_c, PF01751 Toprim
- InterPro
- DNA_gyrase_B_C, HATPase_C_sf, HATPase_dom, Ribosomal_Su5_D2-typ_SF, Ribsml_uS5_D2-typ_fold_subgr, Topo_IIA, Topo_IIA-like_dom_sf, Topo_IIA_B_C, Topo_IIA_bsu_dom2, TopoIIA_CS, TopoIV_B_Gneg, TOPRIM_dom
- Functional cluster
- S-Adenosylmethionine Synthases & related
Experimental structures · PDB · 4
A predicted model is available from AlphaFold.
Gene Ontology · 12
- GO:0005694 chromosome
- GO:0005829 cytosol
- GO:0005524 ATP binding
- GO:0003677 DNA binding
- GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
- GO:0046872 metal ion binding
- GO:0051276 chromosome organization
- GO:0007059 chromosome segregation
- GO:0006265 DNA topological change
- GO:0030541 plasmid partitioning
- GO:0046677 response to antibiotic
- GO:0007062 sister chromatid cohesion
Drugs targeting this protein · 21
- ALATROFLOXACIN MESYLATE inhibitor
- MOXIFLOXACIN HYDROCHLORIDE inhibitor
- TROVAFLOXACIN MESYLATE inhibitor
- BESIFLOXACIN HYDROCHLORIDE inhibitor
- CIPROFLOXACIN HYDROCHLORIDE inhibitor
- NEMONOXACIN inhibitor
- PRADOFLOXACIN inhibitor
- ACORAFLOXACIN inhibitor
- DELAFLOXACIN MEGLUMINE inhibitor
- ZABOFLOXACIN inhibitor
- PEFLOXACIN inhibitor
- GARENOXACIN MESYLATE inhibitor
- GEPOTIDACIN inhibitor
- NADIFLOXACIN inhibitor
- SITAFLOXACIN inhibitor
- PRULIFLOXACIN inhibitor
- TROVAFLOXACIN inhibitor
- RIFAQUIZINONE inhibitor
- CIPROFLOXACIN inhibitor
- SPARFLOXACIN inhibitor
- NORFLOXACIN inhibitor
Related proteins · sequence + function similarity
- DNA topoisomerase 4 subunit B 0.99
- DNA topoisomerase 4 subunit B 0.99
- DNA topoisomerase 4 subunit B 0.89
- DNA topoisomerase 4 subunit B 0.88
- DNA gyrase subunit B 0.81
- DNA gyrase subunit B 0.81
- DNA gyrase subunit B 0.81
- DNA gyrase subunit B 0.81
- DNA gyrase subunit B 0.81
- DNA gyrase subunit B 0.79
- DNA topoisomerase 4 subunit B 0.79
- DNA gyrase subunit B 0.79
Co-cited proteins · studied together in the literature
- DNA topoisomerase 4 subunit A 5 shared papers
- DNA topoisomerase 4 subunit B 1 shared papers
- DNA topoisomerase 4 subunit B 2 shared papers
- DNA topoisomerase 4 subunit B 1 shared papers
- DNA gyrase subunit B 3 shared papers
- DNA gyrase subunit B 1 shared papers
- DNA gyrase subunit A 2 shared papers
- Chromosome partition protein MukF 1 shared papers
- Acyl carrier protein 1 shared papers
- Chromosome partition protein MukB 1 shared papers
- Chromosome partition protein MukE 1 shared papers
Literature · 12 cited papers
- Pyrrolopyrimidine inhibitors of DNA gyrase B (GyrB) and topoisomerase IV (ParE). Part I: Structure guided discovery and optimization of dual targeting agents with potent, broad-spectrum enzymatic activity. Bioorg. Med. Chem. Lett. · 2013
- Pyrrolopyrimidine inhibitors of DNA gyrase B (GyrB) and topoisomerase IV (ParE), Part II: development of inhibitors with broad spectrum, Gram-negative antibacterial activity. Bioorg. Med. Chem. Lett. · 2013
- Use of divalent metal ions in the DNA cleavage reaction of topoisomerase IV. Nucleic Acids Res. · 2011
- Escherichia coli condensin MukB stimulates topoisomerase IV activity by a direct physical interaction. Proc. Natl. Acad. Sci. U.S.A. · 2010
- Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol. Syst. Biol. · 2006
- Crystal structures of Escherichia coli topoisomerase IV ParE subunit (24 and 43 kilodaltons): a single residue dictates differences in novobiocin potency against topoisomerase IV and DNA gyrase. Antimicrob. Agents Chemother. · 2004
- Topoisomerase IV, not gyrase, decatenates products of site-specific recombination in Escherichia coli. Genes Dev. · 1997
- The complete genome sequence of Escherichia coli K-12. Science · 1997
- Quinolone resistance locus nfxD of Escherichia coli is a mutant allele of the parE gene encoding a subunit of topoisomerase IV. Antimicrob. Agents Chemother. · 1997
- Molecular characterization of the Salmonella typhimurium parE gene. Nucleic Acids Res. · 1993
- Escherichia coli topoisomerase IV. Purification, characterization, subunit structure, and subunit interactions. J. Biol. Chem. · 1993
- New topoisomerase essential for chromosome segregation in E. coli. Cell · 1990
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