DNA topoisomerase 4 subunit A

Function

Topoisomerase IV is essential for chromosome segregation; it is the principal protein responsible for decatenating newly replicated chromosomes. It relaxes supercoiled DNA. MukB stimulates the relaxation activity of topoisomerase IV and also has a modest effect on decatenation.

Classification

Family (Pfam)

PF03989 DNA_gyraseA_C, PF00521 DNA_topoisoIV

InterPro

GyrA/parC_rep, Gyrase/topoIV_suA_C, Topo_IIA-like_dom_sf, Topo_IIA_A/C_ab, Topo_IIA_A_a_sf, Topo_IIA_dom_A, TopoIV_A_Gneg, Type_II_DNA_Topoisomerases

Functional cluster

Mixed Regulatory & Membrane Proteins

Experimental structures · PDB · 3

• 1ZVT X-ray 1.70A
• 1ZVU X-ray 3.00A
• 4MN4 X-ray 2.30A

A predicted model is available from AlphaFold.

Gene Ontology · 12

• GO:0005694 chromosome
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0009330 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
• GO:0019897 extrinsic component of plasma membrane
• GO:0005524 ATP binding
• GO:0003677 DNA binding
• GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• GO:0007059 chromosome segregation
• GO:0006265 DNA topological change
• GO:0030541 plasmid partitioning
• GO:0007062 sister chromatid cohesion

Drugs targeting this protein · 21

• ALATROFLOXACIN MESYLATE inhibitor
• MOXIFLOXACIN HYDROCHLORIDE inhibitor
• TROVAFLOXACIN MESYLATE inhibitor
• BESIFLOXACIN HYDROCHLORIDE inhibitor
• CIPROFLOXACIN HYDROCHLORIDE inhibitor
• NEMONOXACIN inhibitor
• PRADOFLOXACIN inhibitor
• ACORAFLOXACIN inhibitor
• DELAFLOXACIN MEGLUMINE inhibitor
• ZABOFLOXACIN inhibitor
• PEFLOXACIN inhibitor
• GARENOXACIN MESYLATE inhibitor
• GEPOTIDACIN inhibitor
• NADIFLOXACIN inhibitor
• SITAFLOXACIN inhibitor
• PRULIFLOXACIN inhibitor
• TROVAFLOXACIN inhibitor
• RIFAQUIZINONE inhibitor
• CIPROFLOXACIN inhibitor
• SPARFLOXACIN inhibitor
• NORFLOXACIN inhibitor

Related proteins · sequence + function similarity

• DNA topoisomerase 4 subunit A 1.00
• DNA topoisomerase 4 subunit A 0.99
• DNA topoisomerase 4 subunit A 0.94
• DNA topoisomerase 4 subunit A 0.93
• DNA topoisomerase 4 subunit A 0.82
• DNA topoisomerase 4 subunit A 0.75
• DNA topoisomerase 4 subunit A 0.75
• DNA topoisomerase 4 subunit A 0.74
• DNA topoisomerase 4 subunit A 0.74
• DNA topoisomerase 4 subunit A 0.70
• DNA topoisomerase 4 subunit A 0.70
• DNA topoisomerase 4 subunit A 0.69

Co-cited proteins · studied together in the literature

• DNA topoisomerase 4 subunit B 5 shared papers
• 1-acyl-sn-glycerol-3-phosphate acyltransferase 1 shared papers
• Cell division protein FtsP 1 shared papers
• Chromosome partition protein MukF 1 shared papers
• Acyl carrier protein 1 shared papers
• Chromosome partition protein MukB 1 shared papers
• Chromosome partition protein MukE 1 shared papers
• DNA gyrase subunit B 1 shared papers
• DNA gyrase subunit A 1 shared papers

Literature · 10 cited papers

• Use of divalent metal ions in the DNA cleavage reaction of topoisomerase IV. Nucleic Acids Res. · 2011
• Escherichia coli condensin MukB stimulates topoisomerase IV activity by a direct physical interaction. Proc. Natl. Acad. Sci. U.S.A. · 2010
• Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol. Syst. Biol. · 2006
• The structural basis for substrate specificity in DNA topoisomerase IV. J. Mol. Biol. · 2005
• The Glu-84 of the ParC subunit plays critical roles in both topoisomerase IV-quinolone and topoisomerase IV-DNA interactions. Biochemistry · 2002
• Topoisomerase IV, not gyrase, decatenates products of site-specific recombination in Escherichia coli. Genes Dev. · 1997
• The complete genome sequence of Escherichia coli K-12. Science · 1997
• Escherichia coli topoisomerase IV. Purification, characterization, subunit structure, and subunit interactions. J. Biol. Chem. · 1993
• New topoisomerase essential for chromosome segregation in E. coli. Cell · 1990
• Characterization of the Escherichia coli gene for 1-acyl-sn-glycerol-3-phosphate acyltransferase (plsC). Mol. Gen. Genet. · 1992