Small ribosomal subunit protein uS5

Function

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. Plays a role in the assembly and function of the 40S ribosomal subunit (By similarity). Mutations in this protein affects the control of translational fidelity (By similarity). Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly (By similarity).

Classification

Family (Pfam)

PF00333 Ribosomal_S5, PF03719 Ribosomal_S5_C

InterPro

Ribosomal_Su5_D2-typ_SF, Ribosomal_uS5, Ribosomal_uS5_C, Ribosomal_uS5_euk_arc, Ribosomal_uS5_N, Ribosomal_uS5_N_CS, Ribsml_uS5_D2-typ_fold_subgr

Functional cluster

RuBisCO & Carbon-Fixation Enzymes

Experimental structures · PDB · 172

• 4UG0 EM
• 4V6X EM 5.00A
• 5A2Q EM 3.90A
• 5AJ0 EM 3.50A
• 5FLX EM 3.90A
• 5LKS EM 3.60A
• 5OA3 EM 4.30A
• 5T2C EM 3.60A
• 5VYC X-ray 6.00A
• 6G18 EM 3.60A
• 6G4S EM 4.00A
• 6G51 EM 4.10A
• … and 160 more

A predicted model is available from AlphaFold.

Gene Ontology · 18

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0070062 extracellular exosome
• GO:0005925 focal adhesion
• GO:0016020 membrane
• GO:0005654 nucleoplasm
• GO:0005634 nucleus
• GO:0045296 cadherin binding
• GO:0019899 enzyme binding
• GO:0017134 fibroblast growth factor binding
• GO:0003729 mRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0002181 cytoplasmic translation
• GO:0051443 positive regulation of ubiquitin-protein transferase activity
• GO:0006412 translation

Drugs targeting this protein · 2

• CYCLOHEXIMIDE inhibitor
• ATALUREN modulator

Related proteins · sequence + function similarity

• Small ribosomal subunit protein uS5 1.00
• Small ribosomal subunit protein uS5 0.99
• Small ribosomal subunit protein uS5 0.99
• Small ribosomal subunit protein uS5 0.98
• Small ribosomal subunit protein uS5 0.94
• Small ribosomal subunit protein uS5 0.93
• Small ribosomal subunit protein uS5 0.93
• Small ribosomal subunit protein uS5y/uS5u/uS5v 0.92
• Small ribosomal subunit protein uS5z 0.92
• Small ribosomal subunit protein uS5x 0.92
• Small ribosomal subunit protein uS5w 0.92
• Small ribosomal subunit protein uS5 0.90

Co-cited proteins · studied together in the literature

• uS5 assembly chaperone PDCD2 2 shared papers
• Protein arginine N-methyltransferase 3 1 shared papers
• Zinc finger protein 277 1 shared papers
• E3 ubiquitin-protein ligase RNF10 4 shared papers
• Small ribosomal subunit protein uS3 8 shared papers
• uS5 assembly chaperone PDCD2L 1 shared papers
• Protein arginine N-methyltransferase 3 2 shared papers
• Serine/threonine-protein kinase RIO3 2 shared papers
• Ubiquitin carboxyl-terminal hydrolase 10 4 shared papers
• Small ribosomal subunit protein eS10 4 shared papers
• Small ribosomal subunit protein uS10 3 shared papers
• OTU domain-containing protein 3 1 shared papers

Literature · 30 cited papers

• RIOK3 mediates the degradation of 40S ribosomes. Mol. Cell · 2025
• The integrated stress response regulates 18S nonfunctional rRNA decay in mammals. Mol. Cell · 2025
• iRQC, a surveillance pathway for 40S ribosomal quality control during mRNA translation initiation. Cell Rep. · 2021
• The E3 ubiquitin ligase RNF10 modifies 40S ribosomal subunits of ribosomes compromised in translation. Cell Rep. · 2021
• PDCD2 functions as an evolutionarily conserved chaperone dedicated for the 40S ribosomal protein uS5 (RPS2). Nucleic Acids Res. · 2020
• Distinct regulatory ribosomal ubiquitylation events are reversible and hierarchically organized. Elife · 2020
• The G3BP1-family-USP10 deubiquitinase complex rescues ubiquitinated 40S subunits of ribosomes stalled in translation from lysosomal degradation. Mol. Cell · 2020
• The 40S ribosomal protein uS5 (RPS2) assembles into an extraribosomal complex with human ZNF277 that competes with the PRMT3-uS5 interaction. J. Biol. Chem. · 2019
• ZNF598 and RACK1 regulate mammalian ribosome-associated quality control function by mediating regulatory 40S ribosomal ubiquitylation. Mol. Cell · 2017
• Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation. Nat. Struct. Mol. Biol. · 2017
• Human PDCD2L Is an Export Substrate of CRM1 That Associates with 40S Ribosomal Subunit Precursors. Mol. Cell. Biol. · 2016
• N-terminome analysis of the human mitochondrial proteome. Proteomics · 2015
• … and 18 more in the literature graph