Monocyte differentiation antigen CD14

Function

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-).

Classification

Family (Pfam)

PF13516 LRR_6

InterPro

Leu-rich_rpt, LRR_dom_sf, Monocyte_diff_Ag_CD14

Functional cluster

Secreted Growth Factors & Cytokines

Experimental structures · PDB · 1

• 4GLP X-ray 4.00A

A predicted model is available from AlphaFold.

Gene Ontology · 37

• GO:0010008 endosome membrane
• GO:0009897 external side of plasma membrane
• GO:0070062 extracellular exosome
• GO:0005576 extracellular region
• GO:0005615 extracellular space
• GO:0005794 Golgi apparatus
• GO:0046696 lipopolysaccharide receptor complex
• GO:0045121 membrane raft
• GO:0005886 plasma membrane
• GO:0030667 secretory granule membrane
• GO:0001530 lipopolysaccharide binding
• GO:0001875 lipopolysaccharide immune receptor activity
• GO:0070891 lipoteichoic acid binding
• GO:0140104 molecular carrier activity
• GO:0001847 opsonin receptor activity
• GO:0016019 peptidoglycan immune receptor activity
• GO:0006915 apoptotic process
• GO:0002752 cell surface pattern recognition receptor signaling pathway
• GO:0007166 cell surface receptor signaling pathway
• GO:0071726 cellular response to diacyl bacterial lipopeptide
• GO:0071222 cellular response to lipopolysaccharide
• GO:0071223 cellular response to lipoteichoic acid
• GO:0071219 cellular response to molecule of bacterial origin
• GO:0071727 cellular response to triacyl bacterial lipopeptide
• GO:0006954 inflammatory response
• GO:0045087 innate immune response
• GO:0006909 phagocytosis
• GO:0001819 positive regulation of cytokine production
• GO:0045807 positive regulation of endocytosis
• GO:0032757 positive regulation of interleukin-8 production
• GO:0031666 positive regulation of lipopolysaccharide-mediated signaling pathway
• GO:0034145 positive regulation of toll-like receptor 4 signaling pathway
• GO:0032760 positive regulation of tumor necrosis factor production
• GO:0032481 positive regulation of type I interferon production
• GO:0032729 positive regulation of type II interferon production
• GO:0006898 receptor-mediated endocytosis
• GO:0034142 toll-like receptor 4 signaling pathway

Drugs targeting this protein · 1

• VB-201 inhibitor

Related proteins · sequence + function similarity

• Monocyte differentiation antigen CD14 0.93
• Monocyte differentiation antigen CD14 0.93
• Monocyte differentiation antigen CD14 0.93
• Monocyte differentiation antigen CD14 0.93
• Transforming growth factor beta activator LRRC33 0.82
• Transforming growth factor beta activator LRRC33 0.81
• Transforming growth factor beta activator LRRC33 0.80
• Toll-like receptor 12 0.79
• Transforming growth factor beta activator LRRC32 0.79
• Transforming growth factor beta activator LRRC33 0.78
• Transforming growth factor beta activator LRRC32 0.78
• Tsukushi 0.77

Co-cited proteins · studied together in the literature

• Monocyte differentiation antigen CD14 2 shared papers
• Lipopolysaccharide-binding protein 2 shared papers
• Follistatin-related protein 1 1 shared papers
• Toll-like receptor 4 3 shared papers
• Lymphocyte antigen 96 2 shared papers
• Lymphocyte antigen 96 1 shared papers
• Pulmonary surfactant-associated protein A 1 shared papers
• Toll-like receptor 6 2 shared papers
• Toll-like receptor 1 2 shared papers
• Macrophage scavenger receptor types I and II 1 shared papers
• Unconventional myosin-XVIIIa 1 shared papers
• Toll-like receptor 4 1 shared papers

Literature · 22 cited papers

• SP-R210 (Myo18A) isoforms as intrinsic modulators of macrophage priming and activation. PLoS ONE · 2015
• 27-Hydroxycholesterol up-regulates CD14 and predisposes monocytic cells to superproduction of CCL2 in response to lipopolysaccharide. Biochim. Biophys. Acta · 2015
• An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
• CD14 and TLR4 mediate cytokine release promoted by electronegative LDL in monocytes. Atherosclerosis · 2013
• The crystal structure of human soluble CD14 reveals a bent solenoid with a hydrophobic amino-terminal pocket. J. Immunol. · 2013
• Follistatin-related protein/follistatin-like 1 evokes an innate immune response via CD14 and toll-like receptor 4. FEBS Lett. · 2012
• Lipopolysaccharide-binding protein-mediated Toll-like receptor 4 dimerization enables rapid signal transduction against lipopolysaccharide stimulation on membrane-associated CD14-expressing cells. Int. Immunol. · 2010
• Enrichment of glycopeptides for glycan structure and attachment site identification. Nat. Methods · 2009
• Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J. Proteome Res. · 2009
• Natural selection in the TLR-related genes in the course of primate evolution. Immunogenetics · 2008
• Membrane sorting of toll-like receptor (TLR)-2/6 and TLR2/1 heterodimers at the cell surface determines heterotypic associations with CD36 and intracellular targeting. J. Biol. Chem. · 2006
• Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. J. Proteome Res. · 2005
• … and 10 more in the literature graph