Toll-like receptor 4
Also known as: TLR4
Function
Transmembrane receptor that functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways. At the plasma membrane, cooperates with LY96 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Mechanistically, acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Alternatively, CD14-mediated TLR4 internalization via endocytosis is associated with the initiation of a MYD88-independent signaling via the TICAM1-TBK1-IRF3 axis leading to type I interferon production. In addition to the secretion of proinflammatory cytokines, initiates the activation of NLRP3 inflammasome and formation of a positive feedback loop between autophagy and NF-kappa-B signaling cascade. In complex with TLR6, promotes inflammation in monocytes/macrophages by associating with TLR6 and the receptor CD86. Upon ligand binding, such as oxLDL or amyloid-beta 42, the TLR4:TLR6 complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway. In myeloid dendritic cells, vesicular stomatitis virus glycoprotein G but not LPS promotes the activation of IRF7, leading to type I IFN production in a CD14-dependent manner. Required for the migration-promoting effects of ZG16B/PAUF on pancreatic cancer cells.
Classification
- Family (Pfam)
- PF12799 LRR_4, PF13516 LRR_6, PF13855 LRR_8, PF01582 TIR
- InterPro
- Cys-rich_flank_reg_C, Leu-rich_rpt, Leu-rich_rpt_4, Leu-rich_rpt_typical-subtyp, LRR_dom_sf, TIR_dom, Toll-like_receptor, Toll_tir_struct_dom_sf
- Functional cluster
- Immunoglobulin-Domain Cell Adhesion Proteins
Experimental structures · PDB · 15
- 2Z62 X-ray 1.70A
- 2Z63 X-ray 2.00A
- 2Z65 X-ray 2.70A
- 2Z66 X-ray 1.90A
- 3FXI X-ray 3.10A
- 3UL7 X-ray 2.37A
- 3UL8 X-ray 2.50A
- 3UL9 X-ray 2.45A
- 3ULA X-ray 3.60A
- 4G8A X-ray 2.40A
- 5NAM NMR
- 5NAO NMR
- … and 3 more
A predicted model is available from AlphaFold.
Gene Ontology · 76
- GO:0009986 cell surface
- GO:0005737 cytoplasm
- GO:0005769 early endosome
- GO:0010008 endosome membrane
- GO:0009897 external side of plasma membrane
- GO:0046696 lipopolysaccharide receptor complex
- GO:0048471 perinuclear region of cytoplasm
- GO:0001891 phagocytic cup
- GO:0005886 plasma membrane
- GO:0043235 receptor complex
- GO:0001726 ruffle
- GO:0001540 amyloid-beta binding
- GO:0042802 identical protein binding
- GO:0001530 lipopolysaccharide binding
- GO:0001875 lipopolysaccharide immune receptor activity
- GO:0046982 protein heterodimerization activity
- GO:0038023 signaling receptor activity
- GO:0005102 signaling receptor binding
- GO:0004888 transmembrane signaling receptor activity
- GO:1904646 cellular response to amyloid-beta
- GO:0071222 cellular response to lipopolysaccharide
- GO:0071260 cellular response to mechanical stimulus
- GO:0140052 cellular response to oxidised low-density lipoprotein particle stimulus
- GO:0036120 cellular response to platelet-derived growth factor stimulus
- GO:0071346 cellular response to type II interferon
- GO:0042742 defense response to bacterium
- GO:0050829 defense response to Gram-negative bacterium
- GO:0016046 detection of fungus
- GO:0032497 detection of lipopolysaccharide
- GO:0006955 immune response
- GO:0006954 inflammatory response
- GO:0045087 innate immune response
- GO:0060729 intestinal epithelial structure maintenance
- GO:0031663 lipopolysaccharide-mediated signaling pathway
- GO:0042116 macrophage activation
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0120163 negative regulation of cold-induced thermogenesis
- GO:0070373 negative regulation of ERK1 and ERK2 cascade
- GO:0032700 negative regulation of interleukin-17 production
- GO:0032707 negative regulation of interleukin-23 production
- GO:0032715 negative regulation of interleukin-6 production
- GO:0045671 negative regulation of osteoclast differentiation
- GO:0032720 negative regulation of tumor necrosis factor production
- GO:0032689 negative regulation of type II interferon production
- GO:0006909 phagocytosis
- GO:0043123 positive regulation of canonical NF-kappaB signal transduction
- GO:1903974 positive regulation of cellular response to macrophage colony-stimulating factor stimulus
- GO:2000343 positive regulation of chemokine (C-X-C motif) ligand 2 production
- GO:0032722 positive regulation of chemokine production
- GO:1900017 positive regulation of cytokine production involved in inflammatory response
- GO:2001238 positive regulation of extrinsic apoptotic signaling pathway
- GO:0010628 positive regulation of gene expression
- GO:0050729 positive regulation of inflammatory response
- GO:0032727 positive regulation of interferon-alpha production
- GO:0032728 positive regulation of interferon-beta production
- GO:0032731 positive regulation of interleukin-1 beta production
- GO:0032732 positive regulation of interleukin-1 production
- GO:0032733 positive regulation of interleukin-10 production
- GO:0032735 positive regulation of interleukin-12 production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0032757 positive regulation of interleukin-8 production
- GO:0043032 positive regulation of macrophage activation
- GO:1904466 positive regulation of matrix metallopeptidase secretion
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:1900227 positive regulation of NLRP3 inflammasome complex assembly
- GO:0010572 positive regulation of platelet activation
- GO:1903428 positive regulation of reactive oxygen species biosynthetic process
- GO:0045944 positive regulation of transcription by RNA polymerase II
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0032729 positive regulation of type II interferon production
- GO:0032496 response to lipopolysaccharide
- GO:0042088 T-helper 1 type immune response
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0002224 toll-like receptor signaling pathway
- GO:0035666 TRIF-dependent toll-like receptor signaling pathway
- GO:0002246 wound healing involved in inflammatory response
Drugs targeting this protein · 2
- RESATORVID inhibitor
- ERITORAN TETRASODIUM antagonist
Related proteins · sequence + function similarity
- Toll-like receptor 4 1.00
- Toll-like receptor 4 1.00
- Toll-like receptor 4 1.00
- Toll-like receptor 4 0.99
- Toll-like receptor 4 0.98
- Toll-like receptor 4 0.98
- Toll-like receptor 4 0.98
- Toll-like receptor 4 0.98
- Toll-like receptor 4 0.98
- Toll-like receptor 4 0.97
- Toll-like receptor 4 0.96
- Toll-like receptor 4 0.96
Co-cited proteins · studied together in the literature
- Mitogen-activated protein kinase kinase kinase 21 1 shared papers
- Triggering receptor expressed on myeloid cells 1 2 shared papers
- CD180 antigen 1 shared papers
- Toll-like receptor 4 7 shared papers
- Toll-like receptor 4 1 shared papers
- Toll-like receptor 4 1 shared papers
- Monocyte differentiation antigen CD14 3 shared papers
- TRAF3-interacting JNK-activating modulator 1 shared papers
- Lymphocyte antigen 96 3 shared papers
- Toll-like receptor 1 5 shared papers
- CD82 antigen 1 shared papers
- TIR domain-containing adapter molecule 2 2 shared papers
Literature · 49 cited papers
- Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-kappaB. Cell · 2024
- A mosquito salivary protein-driven influx of myeloid cells facilitates flavivirus transmission. EMBO J. · 2024
- RNF128 regulates neutrophil infiltration and myeloperoxidase functions to prevent acute lung injury. Cell Death Dis. · 2023
- Recombinant human KAI1/CD82 attenuates M1 macrophage polarization on LPS-stimulated RAW264.7 cells via blocking TLR4/JNK/NF-kappaB signal pathway. BMB Rep. · 2023
- PAUF Induces Migration of Human Pancreatic Cancer Cells Exclusively via the TLR4/MyD88/NF-kappaB Signaling Pathway. Int. J. Mol. Sci. · 2022
- Mycoplasma pneumoniae lipids license TLR-4 for activation of NLRP3 inflammasome and autophagy to evoke a proinflammatory response. Clin. Exp. Immunol. · 2021
- TRAF3-interacting JNK-activating modulator promotes inflammation by stimulating translocation of Toll-like receptor 4 to lipid rafts. J. Biol. Chem. · 2019
- NMI and IFP35 serve as proinflammatory DAMPs during cellular infection and injury. Nat. Commun. · 2017
- The SARM1 Toll/Interleukin-1 receptor domain possesses intrinsic NAD+ cleavage activity that promotes pathological axonal degeneration. Neuron · 2017
- Raftlin controls lipopolysaccharide-induced TLR4 internalization and TICAM-1 signaling in a cell type-specific manner. J. Immunol. · 2016
- Neomorphic effects of recurrent somatic mutations in Yin Yang 1 in insulin-producing adenomas. Proc. Natl. Acad. Sci. U.S.A. · 2015
- WDFY1 mediates TLR3/4 signaling by recruiting TRIF. EMBO Rep. · 2015
- … and 37 more in the literature graph