RNA-directed RNA polymerase catalytic subunit

Function

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.

Classification

Family (Pfam)

PF00602 Flu_PB1

InterPro

RNA-dir_pol_NSvirus, RNA_pol_PB1_influenza

Functional cluster

S-Adenosylmethionine Synthases & related

Experimental structures · PDB · 4

• 2ZNL X-ray 2.30A
• 2ZTT X-ray 2.10A
• 3A1G X-ray 1.70A
• 9IM6 EM 3.21A

A predicted model is available from AlphaFold.

Gene Ontology · 11

• GO:0005576 extracellular region
• GO:0030430 host cell cytoplasm
• GO:0042025 host cell nucleus
• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-directed RNA polymerase activity
• GO:0006351 DNA-templated transcription
• GO:0039657 symbiont-mediated suppression of host gene expression
• GO:0039523 symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
• GO:0039694 viral RNA genome replication
• GO:0019083 viral transcription

Drugs targeting this protein · 1

• FAVIPIRAVIR inhibitor

Related proteins · sequence + function similarity

• RNA-directed RNA polymerase catalytic subunit 1.00
• RNA-directed RNA polymerase catalytic subunit 1.00
• RNA-directed RNA polymerase catalytic subunit 1.00
• RNA-directed RNA polymerase catalytic subunit 1.00
• RNA-directed RNA polymerase catalytic subunit 1.00
• RNA-directed RNA polymerase catalytic subunit 1.00
• RNA-directed RNA polymerase catalytic subunit 1.00
• RNA-directed RNA polymerase catalytic subunit 1.00
• RNA-directed RNA polymerase catalytic subunit 1.00
• RNA-directed RNA polymerase catalytic subunit 1.00
• RNA-directed RNA polymerase catalytic subunit 1.00
• RNA-directed RNA polymerase catalytic subunit 1.00

Co-cited proteins · studied together in the literature

• Protein PB1-F2 2 shared papers
• Polymerase acidic protein 4 shared papers
• Polymerase basic protein 2 3 shared papers
• Hemagglutinin 2 shared papers
• PB2-S1 2 shared papers
• Nucleoprotein 2 shared papers
• Protein PA-X 2 shared papers
• Neuraminidase 2 shared papers
• Nuclear export protein 2 shared papers
• Matrix protein 2 2 shared papers
• Non-structural protein 1 2 shared papers
• Matrix protein 1 2 shared papers

Literature · 10 cited papers

• Comparative influenza protein interactomes identify the role of plakophilin 2 in virus restriction. Nat. Commun. · 2017
• The RNA polymerase of influenza a virus: mechanisms of viral transcription and replication. Acta Virol. · 2013
• Nuclear import and assembly of influenza A virus RNA polymerase studied in live cells by fluorescence cross-correlation spectroscopy. J. Virol. · 2010
• Structural insight into the essential PB1-PB2 subunit contact of the influenza virus RNA polymerase. EMBO J. · 2009
• Influenza A virus proteins PB1 and NS1 are subject to functionally important phosphorylation by protein kinase C. J. Gen. Virol. · 2009
• The structural basis for an essential subunit interaction in influenza virus RNA polymerase. Nature · 2008
• Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments. Virus Res. · 2004
• Plasmid-only rescue of influenza A virus vaccine candidates. Philos. Trans. R. Soc. Lond., B, Biol. Sci. · 2001
• Influenza virus PB1 protein is the minimal and essential subunit of RNA polymerase. Arch. Virol. · 1996
• Nucleotide sequence of human influenza A/PR/8/34 segment 2. Nucleic Acids Res. · 1982