Polymerase basic protein 2

UniProt P03428 Organism Influenza A virus (strain A/Puerto Rico/8/1934 H1N1) Gene PB2

Also known as: PB2

Function

Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. In addition, participates in the inhibition of type I interferon induction through interaction with and inhibition of the host mitochondrial antiviral signaling protein MAVS.

Classification

Family (Pfam): PF20947 Flu_PB2_1st, PF20948 Flu_PB2_2nd, PF20949 Flu_PB2_3rd, PF20950 Flu_PB2_4th, PF00604 Flu_PB2_5th, PF20951 Flu_PB2_6th, PF20952 Flu_PB2_7th
InterPro: Flu_PB2_2nd, Flu_PB2_6th, Flu_PB2_C, Flu_PB2_CAP-bd, Flu_PB2_middle, Flu_PB2_N, INV_PB2, PB2_helical, PDB2_C
Functional cluster: Mixed Regulatory & Membrane Proteins

Experimental structures · PDB · 10

2ZTT X-ray 2.10A
3A1G X-ray 1.70A
3CW4 X-ray 2.70A
3WI0 X-ray 2.00A
3WI1 X-ray 1.93A
4ENF X-ray 1.32A
4J2R X-ray 2.42A
4U6O X-ray 1.30A
7JYW X-ray 2.90A
7JYX X-ray 2.95A

A predicted model is available from AlphaFold.

Gene Ontology · 13

Drugs targeting this protein · 2

FAVIPIRAVIR inhibitor
PIMODIVIR inhibitor

Related proteins · sequence + function similarity

Co-cited proteins · studied together in the literature

Polymerase acidic protein 1 shared papers
Polymerase basic protein 2 1 shared papers
PB2-S1 3 shared papers
Protein PA-X 3 shared papers
Polymerase acidic protein 4 shared papers
RNA-directed RNA polymerase catalytic subunit 3 shared papers
Hemagglutinin 2 shared papers
Nucleoprotein 2 shared papers
Neuraminidase 2 shared papers
Nuclear export protein 2 shared papers
Matrix protein 2 2 shared papers
Non-structural protein 1 2 shared papers

Literature · 15 cited papers

Comparative influenza protein interactomes identify the role of plakophilin 2 in virus restriction. Nat. Commun. · 2017
An important amino acid in nucleoprotein contributes to influenza A virus replication by interacting with polymerase PB2. Virology · 2014
Crystallization and preliminary X-ray diffraction studies of a surface mutant of the middle domain of PB2 from human influenza A (H1N1) virus. Acta Crystallogr. F · 2014
Conformational polymorphism of m7GTP in crystal structure of the PB2 middle domain from human influenza A virus. PLoS ONE · 2013
Structural and functional characterization of K339T substitution identified in the PB2 subunit cap-binding pocket of influenza A virus. J. Biol. Chem. · 2013
Influenza A polymerase subunit PB2 possesses overlapping binding sites for polymerase subunit PB1 and human MAVS proteins. Virus Res. · 2013
Influenza polymerase activity correlates with the strength of interaction between nucleoprotein and PB2 through the host-specific residue K/E627. PLoS ONE · 2012
The PB2 subunit of the influenza virus RNA polymerase affects virulence by interacting with the mitochondrial antiviral signaling protein and inhibiting expression of beta interferon. J. Virol. · 2010
Structural basis of the influenza A virus RNA polymerase PB2 RNA-binding domain containing the pathogenicity-determinant lysine 627 residue. J. Biol. Chem. · 2009
Role of the influenza virus heterotrimeric RNA polymerase complex in the initiation of replication. J. Gen. Virol. · 2006
Characterization of a mitochondrial-targeting signal in the PB2 protein of influenza viruses. Virology · 2006
The PA subunit is required for efficient nuclear accumulation of the PB1 subunit of the influenza A virus RNA polymerase complex. J. Virol. · 2004
… and 3 more in the literature graph

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