Penicillin-binding protein 1B

UniProt P02919 Organism Escherichia coli (strain K12) Gene JW0145, b0149, mrcB, pbpF, ponB EC 2.4.99.28, 3.4.16.4

Also known as: JW0145, b0149, mrcB, pbpF, ponB

Function

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).

Classification

Family (Pfam): PF14812 PBP1_TM, PF00912 Transgly, PF00905 Transpeptidase, PF14814 UB2H
InterPro: Beta-lactam/transpept-like, Glyco_trans_51, Glycosyltr_51/Transpeptidase, Lysozyme-like_dom_sf, PBP1b_TM, PBP_1b, PBP_transglycosylase, PCN-bd_Tpept, UB2H
Functional cluster: Membrane Insertases & Cytochromes

Experimental structures · PDB · 11

3FWL X-ray 3.09A
3VMA X-ray 2.16A
5FGZ X-ray 2.85A
5HL9 X-ray 2.70A
5HLA X-ray 2.36A
5HLB X-ray 2.42A
5HLD X-ray 2.31A
6FZK NMR
6G5R NMR
6YN0 X-ray 2.40A
7LQ6 EM 3.28A

A predicted model is available from AlphaFold.

Gene Ontology · 13

Drugs targeting this protein · 85

CEFOTAXIME SODIUM inhibitor
AMOXICILLIN inhibitor
CEFUROXIME AXETIL inhibitor
PENICILLIN G SODIUM inhibitor
CEFAMANDOLE inhibitor
CYCLACILLIN inhibitor
MOXALACTAM DISODIUM inhibitor
CEPHALEXIN HYDROCHLORIDE inhibitor
CEFOPERAZONE SODIUM inhibitor
CEFAZOLIN SODIUM inhibitor
CEFOXITIN SODIUM inhibitor
CEPHALEXIN inhibitor
LORACARBEF inhibitor
CEFPIRAMIDE SODIUM inhibitor
AMPICILLIN SODIUM inhibitor
CEFTAZIDIME SODIUM inhibitor
PIPERACILLIN SODIUM inhibitor
PENICILLIN V POTASSIUM inhibitor
TICARCILLIN DISODIUM inhibitor
CEFEPIME HYDROCHLORIDE inhibitor
BACAMPICILLIN HYDROCHLORIDE inhibitor
CEPHALOGLYCIN inhibitor
CEFTIBUTEN DIHYDRATE inhibitor
CARBENICILLIN INDANYL SODIUM inhibitor
CEFONICID SODIUM inhibitor
CEFPODOXIME PROXETIL inhibitor
CEFACLOR inhibitor
CEFTIZOXIME SODIUM inhibitor
CEPHAPIRIN SODIUM inhibitor
CEFORANIDE inhibitor
CEFOTETAN DISODIUM inhibitor
CEFMETAZOLE SODIUM inhibitor
CEFTOLOZANE SULFATE inhibitor
PENICILLIN G POTASSIUM inhibitor
ERTAPENEM SODIUM inhibitor
RAZUPENEM inhibitor
FAROPENEM MEDOXOMIL inhibitor
MEROPENEM inhibitor
TEMOCILLIN inhibitor
ERTAPENEM inhibitor
CEFIXIME inhibitor
FLOMOXEF inhibitor
AZTREONAM inhibitor
CEPHRADINE inhibitor
CEFTIBUTEN inhibitor
PHENETHICILLIN inhibitor
CEFAMANDOLE NAFATE inhibitor
TALAMPICILLIN inhibitor
CEPHALOTHIN SODIUM inhibitor
CEFADROXIL inhibitor
CEFPODOXIME inhibitor
MEZLOCILLIN SODIUM inhibitor
AMPICILLIN inhibitor
SULOPENEM inhibitor
PENAMECILLIN inhibitor
TEBIPENEM PIVOXIL inhibitor
CEFUROXIME SODIUM inhibitor
FLOXACILLIN inhibitor
CEFTIOFUR inhibitor
CEFODIZIME inhibitor
PIVAMPICILLIN inhibitor
CEFMENOXIME HYDROCHLORIDE inhibitor
CEFSULODIN inhibitor
CEFTAROLINE FOSAMIL ACETATE inhibitor
AZTREONAM LYSINE inhibitor
PENICILLIN G BENZATHINE inhibitor
CEFIDEROCOL inhibitor
CEFIDEROCOL SULFATE TOSYLATE inhibitor
CEFILAVANCIN inhibitor
CEFOTIAM HYDROCHLORIDE inhibitor
DORIPENEM MONOHYDRATE inhibitor
IMIPENEM inhibitor
CEFTAZIDIME inhibitor
CEFDITOREN PIVOXIL inhibitor
SULOPENEM ETZADROXIL inhibitor
DORIPENEM inhibitor
CEFTAROLINE FOSAMIL inhibitor
CEFTOBIPROLE inhibitor
CEFPROZIL, (E)- inhibitor
FAROPENEM inhibitor
CARBENICILLIN DISODIUM inhibitor
CEFTRIAXONE SODIUM inhibitor
PENICILLIN V inhibitor
CEFPIROME inhibitor
CEFDINIR inhibitor

Related proteins · sequence + function similarity

Penicillin-binding protein 1B 0.85
Penicillin-binding protein 1A 0.85
Penicillin-binding protein 1B 0.84
Penicillin-binding protein 1A 0.84
Penicillin-binding protein 1A 0.83
Peptidoglycan D,D-transpeptidase FtsI 0.81
Peptidoglycan D,D-transpeptidase FtsI 0.81
Penicillin-binding protein 1A 0.81
Penicillin-binding protein 1A 0.79
Penicillin-binding protein 1A 0.79
Penicillin-binding protein 1A 0.79
Penicillin-binding protein 1A 0.79

Co-cited proteins · studied together in the literature

Penicillin-binding protein 1A 3 shared papers
Penicillin-binding protein 1C 1 shared papers
Membrane-bound lytic murein transglycosylase A 1 shared papers
MltA-interacting protein 1 shared papers

Literature · 14 cited papers

Crystal structure of the membrane-bound bifunctional transglycosylase PBP1b from Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. · 2009
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol. Syst. Biol. · 2006
The catalytic, glycosyl transferase and acyl transferase modules of the cell wall peptidoglycan-polymerizing penicillin-binding protein 1b of Escherichia coli. Mol. Microbiol. · 1999
Demonstration of molecular interactions between the murein polymerase PBP1B, the lytic transglycosylase MltA, and the scaffolding protein MipA of Escherichia coli. J. Biol. Chem. · 1999
Multimodular penicillin-binding proteins: an enigmatic family of orthologs and paralogs. Microbiol. Mol. Biol. Rev. · 1998
The complete genome sequence of Escherichia coli K-12. Science · 1997
Topographical and functional investigation of Escherichia coli penicillin-binding protein 1b by alanine stretch scanning mutagenesis. J. Bacteriol. · 1997
Localization of a putative second membrane association site in penicillin-binding protein 1B of Escherichia coli. Biochem. J. · 1996
Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region. Nucleic Acids Res. · 1994
Functional biosynthesis of cell wall peptidoglycan by polymorphic bifunctional polypeptides. Penicillin-binding protein 1Bs of Escherichia coli with activities of transglycosylase and transpeptidase. J. Biol. Chem. · 1984
Sequences of the active-site peptides of three of the high-Mr penicillin-binding proteins of Escherichia coli K-12. Proc. Natl. Acad. Sci. U.S.A. · 1985
The nucleotide sequences of the ponA and ponB genes encoding penicillin-binding protein 1A and 1B of Escherichia coli K12. Eur. J. Biochem. · 1985
… and 2 more in the literature graph

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