Carbapenem-hydrolyzing beta-lactamase KPC-2

Function

Class A beta-lactamase which confers resistance to the beta-lactam antibiotics, including penicillins, cephalosporins and carbapenems. Acts via hydrolysis of the beta-lactam ring. Has penicillin-, cephalosporin- and carbapenem-hydrolyzing activities.

Classification

Family (Pfam)

PF13354 Beta-lactamase2

InterPro

Beta-lactam/transpept-like, Beta-lactam_cat, Beta-lactam_class-A, KPC-2/SME-1-like

Functional cluster

Oxidoreductases & Polyketide Synthases

Experimental structures · PDB · 83

• 2OV5 X-ray 1.85A
• 3E2K X-ray 2.10A
• 3E2L X-ray 1.87A
• 3RXW X-ray 1.26A
• 3RXX X-ray 1.62A
• 4ZBE X-ray 1.80A
• 5EEC X-ray 1.87A
• 5UJ3 X-ray 1.45A
• 5UJ4 X-ray 1.40A
• 5UL8 X-ray 1.15A
• 6B1F X-ray 1.44A
• 6B1H X-ray 1.80A
• … and 71 more

A predicted model is available from AlphaFold.

Gene Ontology · 4

• GO:0005829 cytosol
• GO:0008800 beta-lactamase activity
• GO:0030655 beta-lactam antibiotic catabolic process
• GO:0046677 response to antibiotic

Drugs targeting this protein · 1

• VABORBACTAM inhibitor

Related proteins · sequence + function similarity

• Carbapenem-hydrolyzing beta-lactamase KPC 1.00
• Beta-lactamase CTX-M-14 0.92
• Beta-lactamase CTX-M-25 0.92
• Beta-lactamase CTX-M-8 0.91
• Beta-lactamase OXY-1 0.90
• Beta-lactamase OXY-2 0.90
• Beta-lactamase 0.90
• Beta-lactamase L2 0.90
• Beta-lactamase CTX-M-15 0.89
• Beta-lactamase CTX-M-6 0.88
• Beta-lactamase CTX-M-1 0.88
• Beta-lactamase CTX-M-4 0.88

Co-cited proteins · studied together in the literature

• Beta-lactamase SHV-29 1 shared papers

Literature · 6 cited papers

• Structural Characterization of the D179N and D179Y Variants of KPC-2 beta-Lactamase: -Loop Destabilization as a Mechanism of Resistance to Ceftazidime-Avibactam. Antimicrob. Agents Chemother. · 2022
• Cyclic boronates as versatile scaffolds for KPC-2 beta-lactamase inhibition. RSC Med. Chem. · 2020
• Natural variants modify Klebsiella pneumoniae carbapenemase (KPC) acyl-enzyme conformational dynamics to extend antibiotic resistance. J. Biol. Chem. · 2021
• Molecular Basis of Substrate Recognition and Product Release by the Klebsiella pneumoniae Carbapenemase (KPC-2). J. Med. Chem. · 2017
• Novel carbapenem-hydrolyzing beta-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae. Antimicrob. Agents Chemother. · 2001
• A standard numbering scheme for the class A beta-lactamases. Biochem. J. · 1991