5'-3' DNA helicase ZGRF1

Function

5'-3' DNA helicase which is recruited to sites of DNA damage and promotes repair of replication-blocking DNA lesions through stimulation of homologous recombination (HR). Promotes HR by directly stimulating RAD51-mediated strand exchange activity. Not required to load RAD51 at sites of DNA damage but promotes recombinational repair after RAD51 recruitment. Also promotes HR by positively regulating EXO1-mediated DNA end resection of double-strand breaks. Required for recruitment of replication protein RPA2 to DNA damage sites. Promotes the initiation of the G2/M checkpoint but not its maintenance. Catalyzes Holliday junction branch migration and dissociation of D-loops and DNA flaps.

Classification

Family (Pfam)

PF13086 AAA_11, PF13087 AAA_12, PF10382 ZGRF1-like_N, PF06839 Zn_ribbon_GRF

InterPro

DNA2/NAM7-like_C, DNA2/NAM7_AAA_11, DNA_Repair_Helicase_ZGRF1, P-loop_NTPase, SF1_C_Upf1, ZGRF1-like_N, Znf_GRF

Functional cluster

Zinc-Finger & Chromatin Regulatory Proteins

Gene Ontology · 8

• GO:0005634 nucleus
• GO:0035861 site of double-strand break
• GO:0043139 5'-3' DNA helicase activity
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0008270 zinc ion binding
• GO:0006302 double-strand break repair
• GO:0000725 recombinational repair

Neighborhood · nearest proteins

Related proteins · sequence + function similarity

• Breast cancer type 2 susceptibility protein homolog 0.92
• 5'-3' DNA helicase ZGRF1 0.92
• Breast cancer type 2 susceptibility protein 0.92
• Breast cancer type 2 susceptibility protein homolog 0.92
• Breast cancer type 1 susceptibility protein homolog 0.91
• Breast cancer type 2 susceptibility protein homolog 0.91
• Breast cancer type 1 susceptibility protein homolog 0.91
• Breast cancer type 1 susceptibility protein homolog 0.91
• Breast cancer type 1 susceptibility protein homolog 0.91
• Breast cancer type 1 susceptibility protein homolog 0.91
• Testis-expressed protein 15 0.91
• Breast cancer type 1 susceptibility protein 0.91

Co-cited proteins · studied together in the literature

• Exonuclease 1 2 shared papers
• DNA repair protein RAD51 homolog 1 1 shared papers
• Breast cancer type 1 susceptibility protein 2 shared papers

Literature · 8 cited papers

• PMID 36400755 Cell. Death. Discov. · 2022
• ZGRF1 promotes end resection of DNA homologous recombination via forming complex with BRCA1/EXO1. Cell. Death. Discov. · 2021
• The ZGRF1 Helicase Promotes Recombinational Repair of Replication-Blocking DNA Damage in Human Cells. Cell Rep. · 2020
• Toward a comprehensive characterization of a human cancer cell phosphoproteome. J. Proteome Res. · 2013
• The full-ORF clone resource of the German cDNA consortium. BMC Genomics · 2007
• Generation and annotation of the DNA sequences of human chromosomes 2 and 4. Nature · 2005
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. · 2004
• Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat. Genet. · 2004