Guanine nucleotide-binding protein subunit alpha-13

Function

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway. Inhibits CDH1-mediated cell adhesion in a process independent from Rho activation. In lymphoid follicles, transmits P2RY8- and S1PR2-dependent signals that lead to inhibition of germinal center (GC) B cell growth and migration outside the GC niche.

Classification

Family (Pfam)

PF00503 G-alpha

InterPro

Gprotein_alpha_12/13, Gprotein_alpha_su, GproteinA_insert, P-loop_NTPase

Functional cluster

Ras-like GTPases & Nucleoid Proteins

Experimental structures · PDB · 15

• 7SF7 EM 2.90A
• 7SF8 EM 2.70A
• 7T6B EM 3.19A
• 7YDH EM 3.10A
• 8H8J EM 3.20A
• 8IKL EM 2.33A
• 8KGG EM 3.06A
• 8ZX4 EM 2.85A
• 8ZX5 EM 3.03A
• 9GE2 EM 2.51A
• 9GE3 EM 2.87A
• 9IY8 EM 3.01A
• … and 3 more

A predicted model is available from AlphaFold.

Gene Ontology · 26

• GO:0031526 brush border membrane
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0070062 extracellular exosome
• GO:0005925 focal adhesion
• GO:0005834 heterotrimeric G-protein complex
• GO:0042470 melanosome
• GO:0016020 membrane
• GO:0005634 nucleus
• GO:0005886 plasma membrane
• GO:0098794 postsynapse
• GO:0031752 D5 dopamine receptor binding
• GO:0003925 G protein activity
• GO:0031683 G-protein beta/gamma-subunit complex binding
• GO:0005525 GTP binding
• GO:0003924 GTPase activity
• GO:0005085 guanyl-nucleotide exchange factor activity
• GO:0046872 metal ion binding
• GO:0007189 adenylate cyclase-activating G protein-coupled receptor signaling pathway
• GO:0007188 adenylate cyclase-modulating G protein-coupled receptor signaling pathway
• GO:0008217 regulation of blood pressure
• GO:0150052 regulation of postsynapse assembly
• GO:0051056 regulation of small GTPase mediated signal transduction
• GO:0007266 Rho protein signal transduction
• GO:0160221 Rho-activating G protein-coupled receptor signaling pathway
• GO:0007165 signal transduction

Neighborhood · nearest proteins

Related proteins · sequence + function similarity

• Guanine nucleotide-binding protein subunit alpha-13 1.00
• Guanine nucleotide-binding protein subunit alpha-13 1.00
• Guanine nucleotide-binding protein alpha-12 subunit 0.90
• Guanine nucleotide-binding protein subunit alpha-12 0.90
• Guanine nucleotide-binding protein alpha-12 subunit 0.89
• Guanine nucleotide-binding protein subunit alpha-12 0.89
• Guanine nucleotide-binding protein alpha-2 subunit 0.88
• Guanine nucleotide-binding protein subunit alpha-12 0.88
• Guanine nucleotide binding protein alpha subunit C 0.88
• Guanine nucleotide-binding protein G(i) subunit alpha 0.86
• Guanine nucleotide-binding protein subunit alpha homolog 0.86
• Guanine nucleotide-binding protein subunit alpha 0.85

Co-cited proteins · studied together in the literature

• Guanine nucleotide-binding protein subunit alpha-12 10 shared papers
• Rho guanine nucleotide exchange factor 1 1 shared papers
• UBX domain-containing protein 11 1 shared papers
• G protein-coupled receptor 35 1 shared papers
• HCLS1-associated protein X-1 1 shared papers
• Guanine nucleotide-binding protein subunit alpha-13 4 shared papers
• Rho guanine nucleotide exchange factor 12 1 shared papers
• Rho guanine nucleotide exchange factor 11 1 shared papers
• Regulator of G protein signaling 22 1 shared papers
• Regulator of G protein signaling 22 1 shared papers
• Adhesion G protein-coupled receptor L3 1 shared papers
• Sphingosine 1-phosphate receptor 2 1 shared papers

Literature · 23 cited papers

• Insights into divalent cation regulation and G13-coupling of orphan receptor GPR35. Cell Discov. · 2022
• The tethered peptide activation mechanism of adhesion GPCRs. Nature · 2022
• Galpha12/13 signaling promotes cervical cancer invasion through the RhoA/ROCK-JNK signaling axis. Biochem. Biophys. Res. Commun. · 2016
• N-terminome analysis of the human mitochondrial proteome. Proteomics · 2015
• Loss of signalling via Galpha13 in germinal centre B-cell-derived lymphoma. Nature · 2014
• A novel Galphas-binding protein, Gas-2 like 2, facilitates the signaling of the A2A adenosine receptor. Biochim. Biophys. Acta · 2013
• Initial characterization of the human central proteome. BMC Syst. Biol. · 2011
• RGS22, a novel testis-specific regulator of G-protein signaling involved in human and mouse spermiogenesis along with GNA12/13 subunits. Biol. Reprod. · 2008
• Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes. J. Proteome Res. · 2006
• A role for the G12 family of heterotrimeric G proteins in prostate cancer invasion. J. Biol. Chem. · 2006
• The G12 family of heterotrimeric G proteins promotes breast cancer invasion and metastasis. Proc. Natl. Acad. Sci. U.S.A. · 2006
• Socius, a novel binding partner of Galpha12/13, promotes the Galpha12-induced RhoA activation. Biochem. Biophys. Res. Commun. · 2005
• … and 11 more in the literature graph