Small ribosomal subunit protein RACK1
Also known as: GNB2L1, RACK1
Function
Scaffolding protein involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Involved in the initiation of the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, by promoting ubiquitination of a subset of 40S ribosomal subunits. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane. Promotes migration of breast carcinoma cells by binding to and activating RHOA. Acts as an adapter for the dephosphorylation and inactivation of AKT1 by promoting recruitment of PP2A phosphatase to AKT1 (By similarity).
Classification
- Family (Pfam)
- PF00400 WD40
- InterPro
- RACK1-like, WD40/YVTN_repeat-like_dom_sf, WD40_PAC1, WD40_repeat_CS, WD40_repeat_dom_sf, WD40_rpt
- Functional cluster
- Histones & Chromatin Proteins
Experimental structures · PDB · 167
- 4AOW X-ray 2.45A
- 4UG0 EM
- 4V5Z EM 8.70A
- 4V6X EM 5.00A
- 5A2Q EM 3.90A
- 5AJ0 EM 3.50A
- 5FLX EM 3.90A
- 5LKS EM 3.60A
- 5OA3 EM 4.30A
- 5T2C EM 3.60A
- 5VYC X-ray 6.00A
- 6FEC EM 6.30A
- … and 155 more
A predicted model is available from AlphaFold.
Gene Ontology · 68
- GO:0005737 cytoplasm
- GO:0005829 cytosol
- GO:0030425 dendrite
- GO:0070062 extracellular exosome
- GO:1990630 IRE1-RACK1-PP2A complex
- GO:0030496 midbody
- GO:0005739 mitochondrion
- GO:0043025 neuronal cell body
- GO:0005654 nucleoplasm
- GO:0005634 nucleus
- GO:0043204 perikaryon
- GO:0048471 perinuclear region of cytoplasm
- GO:0001891 phagocytic cup
- GO:0015935 small ribosomal subunit
- GO:0051434 BH3 domain binding
- GO:0045296 cadherin binding
- GO:0030332 cyclin binding
- GO:0008656 cysteine-type endopeptidase activator activity involved in apoptotic process
- GO:0008047 enzyme activator activity
- GO:0019899 enzyme binding
- GO:0042802 identical protein binding
- GO:0008200 ion channel inhibitor activity
- GO:0060090 molecular adaptor activity
- GO:0042803 protein homodimerization activity
- GO:0005080 protein kinase C binding
- GO:0019903 protein phosphatase binding
- GO:0030291 protein serine/threonine kinase inhibitor activity
- GO:0030292 protein tyrosine kinase inhibitor activity
- GO:0030971 receptor tyrosine kinase binding
- GO:0043022 ribosome binding
- GO:0003723 RNA binding
- GO:0042169 SH2 domain binding
- GO:0035591 signaling adaptor activity
- GO:0005102 signaling receptor binding
- GO:0045182 translation regulator activity
- GO:0071333 cellular response to glucose stimulus
- GO:0071363 cellular response to growth factor stimulus
- GO:0002181 cytoplasmic translation
- GO:0007369 gastrulation
- GO:0030308 negative regulation of cell growth
- GO:1900102 negative regulation of endoplasmic reticulum unfolded protein response
- GO:0010629 negative regulation of gene expression
- GO:1903751 negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide
- GO:0050765 negative regulation of phagocytosis
- GO:0051898 negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
- GO:0032091 negative regulation of protein binding
- GO:0045879 negative regulation of smoothened signaling pathway
- GO:0017148 negative regulation of translation
- GO:2001125 negative regulation of translational frameshifting
- GO:0030178 negative regulation of Wnt signaling pathway
- GO:0043065 positive regulation of apoptotic process
- GO:0030335 positive regulation of cell migration
- GO:2000543 positive regulation of gastrulation
- GO:0042998 positive regulation of Golgi to plasma membrane protein transport
- GO:0043547 positive regulation of GTPase activity
- GO:2001244 positive regulation of intrinsic apoptotic signaling pathway
- GO:0051901 positive regulation of mitochondrial depolarization
- GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
- GO:0001934 positive regulation of protein phosphorylation
- GO:0031334 positive regulation of protein-containing complex assembly
- GO:0016567 protein ubiquitination
- GO:0106070 regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway
- GO:0051726 regulation of cell cycle
- GO:0051302 regulation of cell division
- GO:2000114 regulation of establishment of cell polarity
- GO:0032880 regulation of protein localization
- GO:0072344 rescue of stalled cytosolic ribosome
- GO:0048511 rhythmic process
Neighborhood · nearest proteins
Related proteins · sequence + function similarity
- Small ribosomal subunit protein RACK1 1.00
- Small ribosomal subunit protein RACK1 1.00
- Small ribosomal subunit protein RACK1 1.00
- Small ribosomal subunit protein RACK1 1.00
- Small ribosomal subunit protein RACK1 1.00
- Small ribosomal subunit protein RACK1 1.00
- Small ribosomal subunit protein RACK1 1.00
- Small ribosomal subunit protein RACK1 0.99
- Small ribosomal subunit protein RACK1 0.98
- Small ribosomal subunit protein RACK1 0.98
- Small ribosomal subunit protein RACK1 0.97
- Small ribosomal subunit protein RACK1 0.96
Co-cited proteins · studied together in the literature
- Small ribosomal subunit protein RACK1 1 shared papers
- Sodium bicarbonate cotransporter 3 1 shared papers
- Thromboxane A2 receptor 1 shared papers
- La-related protein 4B 1 shared papers
- Protein Nef 1 shared papers
- Disintegrin and metalloproteinase domain-containing protein 12 1 shared papers
- Protein PA-X 1 shared papers
- Sodium/hydrogen exchanger 5 1 shared papers
- Major surface antigen p30 1 shared papers
- B1 kinase 1 shared papers
- C-type lectin domain family 1 member B 1 shared papers
- Polycystin-2-like protein 1 1 shared papers
Literature · 61 cited papers
- Toxoplasma gondii surface antigen 1 (SAG1) interacts in vitro with host cell receptor for activated C kinase 1 (RACK1). Acta Trop. · 2024
- Proteomic and genetic analyses of influenza A viruses identify pan-viral host targets. Nat. Commun. · 2023
- The matrix protein of respiratory syncytial virus suppresses interferon signaling via RACK1 association. J. Virol. · 2023
- LACC1 deficiency links juvenile arthritis with autophagy and metabolism in macrophages. J. Exp. Med. · 2021
- Trafficking, localization and degradation of the Na+,HCO3- co-transporter NBCn1 in kidney and breast epithelial cells. Sci. Rep. · 2018
- Trans-kingdom mimicry underlies ribosome customization by a poxvirus kinase. Nature · 2017
- Structural analysis of ribosomal RACK1 and its role in translational control. Cell. Signal. · 2017
- ZNF598 and RACK1 regulate mammalian ribosome-associated quality control function by mediating regulatory 40S ribosomal ubiquitylation. Mol. Cell · 2017
- N-terminome analysis of the human mitochondrial proteome. Proteomics · 2015
- RACK1 controls IRES-mediated translation of viruses. Cell · 2014
- A new system for naming ribosomal proteins. Curr. Opin. Struct. Biol. · 2014
- An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
- … and 49 more in the literature graph