DNA gyrase subunit A
Also known as: gyrA
Function
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.
Classification
- Family (Pfam)
- PF03989 DNA_gyraseA_C, PF00521 DNA_topoisoIV
- InterPro
- GyrA, GyrA/parC_rep, Gyrase/topoIV_suA_C, Topo_IIA-like_dom_sf, Topo_IIA_A/C_ab, Topo_IIA_A_a_sf, Topo_IIA_dom_A, Type_II_DNA_Topoisomerases
- Functional cluster
- AAA+ ATPases & Chaperones
Experimental structures · PDB · 5
A predicted model is available from AlphaFold.
Gene Ontology · 9
- GO:0005694 chromosome
- GO:0005737 cytoplasm
- GO:0009330 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
- GO:0005524 ATP binding
- GO:0003677 DNA binding
- GO:0034335 DNA negative supercoiling activity
- GO:0006265 DNA topological change
- GO:0006261 DNA-templated DNA replication
- GO:0046677 response to antibiotic
Drugs targeting this protein · 2
- FINAFLOXACIN inhibitor
- OZENOXACIN inhibitor
Related proteins · sequence + function similarity
- DNA gyrase subunit A 1.00
- DNA gyrase subunit A 1.00
- DNA gyrase subunit A 1.00
- DNA gyrase subunit A 1.00
- DNA gyrase subunit A 1.00
- DNA gyrase subunit A 1.00
- DNA gyrase subunit A 1.00
- DNA gyrase subunit A 1.00
- DNA gyrase subunit A 0.98
- DNA gyrase subunit A 0.98
- DNA gyrase subunit A 0.93
- DNA gyrase subunit A 0.92
Co-cited proteins · studied together in the literature
- DNA gyrase subunit B 4 shared papers
- DNA replication and repair protein RecF 2 shared papers
Literature · 6 cited papers
- Cloning, sequencing, and expression of the DNA gyrase genes from Staphylococcus aureus. J. Bacteriol. · 1993
- Quinolone resistance mutations in the DNA gyrase gyrA and gyrB genes of Staphylococcus aureus. Antimicrob. Agents Chemother. · 1994
- DNA gyrase gyrA mutations in ciprofloxacin-resistant strains of Staphylococcus aureus: close similarity with quinolone resistance mutations in Escherichia coli. J. Bacteriol. · 1990
- DNA cloning and organization of the Staphylococcus aureus gyrA and gyrB genes: close homology among gyrase proteins and implications for 4-quinolone action and resistance. J. Bacteriol. · 1990
- Detection of gyrA gene mutations associated with ciprofloxacin resistance in methicillin-resistant Staphylococcus aureus: analysis by polymerase chain reaction and automated direct DNA sequencing. Antimicrob. Agents Chemother. · 1992
- Nucleotide sequence of the Staphylococcus aureus gyrB-gyrA locus encoding the DNA gyrase A and B proteins. J. Bacteriol. · 1992
A document in the lmmol reference corpus — open public data (UniProt, GO, PDB, the literature graph) rendered as a single cross-linked page. Hover any link to preview its target.