DNA gyrase subunit B
Also known as: gyrB
Function
A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.
Classification
- Family (Pfam)
- PF00204 DNA_gyraseB, PF00986 DNA_gyraseB_C, PF02518 HATPase_c, PF01751 Toprim
- InterPro
- DNA_gyrase_B_C, GyrB, HATPase_C_sf, HATPase_dom, Ribosomal_Su5_D2-typ_SF, Ribsml_uS5_D2-typ_fold_subgr, Topo_IIA, Topo_IIA-like_dom_sf, Topo_IIA_B, Topo_IIA_B_C, Topo_IIA_bsu_dom2, TopoIIA_CS, TOPRIM_dom, TOPRIM_GyrB
- Functional cluster
- S-Adenosylmethionine Synthases & related
Experimental structures · PDB · 32
- 3G75 X-ray 2.30A
- 3G7B X-ray 2.30A
- 3TTZ X-ray 1.63A
- 3U2D X-ray 1.85A
- 3U2K X-ray 1.64A
- 4PLB X-ray 2.69A
- 4URM X-ray 2.94A
- 4URO X-ray 2.59A
- 5BS3 X-ray 2.65A
- 5CPH X-ray 1.20A
- 5CTU X-ray 1.45A
- 5CTW X-ray 1.48A
- … and 20 more
A predicted model is available from AlphaFold.
Gene Ontology · 9
Drugs targeting this protein · 2
- FINAFLOXACIN inhibitor
- OZENOXACIN inhibitor
Related proteins · sequence + function similarity
- DNA gyrase subunit B 1.00
- DNA gyrase subunit B 1.00
- DNA gyrase subunit B 1.00
- DNA gyrase subunit B 1.00
- DNA gyrase subunit B 1.00
- DNA gyrase subunit B 1.00
- DNA gyrase subunit B 0.99
- DNA gyrase subunit B 0.99
- DNA gyrase subunit B 0.97
- DNA gyrase subunit B 0.97
- DNA gyrase subunit B 0.97
- DNA gyrase subunit B 0.97
Co-cited proteins · studied together in the literature
- DNA gyrase subunit A 4 shared papers
- DNA replication and repair protein RecF 3 shared papers
- Chromosomal replication initiator protein DnaA 1 shared papers
- Beta sliding clamp 1 shared papers
- DNA gyrase subunit B 1 shared papers
- DNA gyrase subunit A 1 shared papers
Literature · 6 cited papers
- Discovery of pyrazolthiazoles as novel and potent inhibitors of bacterial gyrase. Bioorg. Med. Chem. Lett. · 2010
- Cloning, sequencing, and expression of the DNA gyrase genes from Staphylococcus aureus. J. Bacteriol. · 1993
- Nucleotide sequence of the recF gene cluster from Staphylococcus aureus and complementation analysis in Bacillus subtilis recF mutants. Mol. Gen. Genet. · 1995
- Quinolone resistance mutations in the DNA gyrase gyrA and gyrB genes of Staphylococcus aureus. Antimicrob. Agents Chemother. · 1994
- DNA cloning and organization of the Staphylococcus aureus gyrA and gyrB genes: close homology among gyrase proteins and implications for 4-quinolone action and resistance. J. Bacteriol. · 1990
- Nucleotide sequence of the Staphylococcus aureus gyrB-gyrA locus encoding the DNA gyrase A and B proteins. J. Bacteriol. · 1992
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