Probable ATP-dependent RNA helicase DDX5

Function

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as a transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.

Classification

Family (Pfam)

PF00270 DEAD, PF00271 Helicase_C, PF08061 P68HR

InterPro

DEAD/DEAH_box_helicase_dom, Helicase_ATP-bd, Helicase_C-like, P-loop_NTPase, P68_rpt, RNA-helicase_DEAD-box_CS, RNA_helicase_DEAD_Q_motif

Functional cluster

Protein Serine/Threonine Kinases

Experimental structures · PDB · 2

• 3FE2 X-ray 2.60A
• 4A4D X-ray 2.70A

A predicted model is available from AlphaFold.

Gene Ontology · 48

• GO:0071013 catalytic step 2 spliceosome
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0070062 extracellular exosome
• GO:0016020 membrane
• GO:0016607 nuclear speck
• GO:0005730 nucleolus
• GO:0005654 nucleoplasm
• GO:0005634 nucleus
• GO:1990904 ribonucleoprotein complex
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity
• GO:0048306 calcium-dependent protein binding
• GO:0005516 calmodulin binding
• GO:0019899 enzyme binding
• GO:0035500 MH2 domain binding
• GO:0003730 mRNA 3'-UTR binding
• GO:0003729 mRNA binding
• GO:0050681 nuclear androgen receptor binding
• GO:0036002 pre-mRNA binding
• GO:0070878 primary miRNA binding
• GO:1990841 promoter-specific chromatin binding
• GO:0070412 R-SMAD binding
• GO:0043021 ribonucleoprotein complex binding
• GO:0003723 RNA binding
• GO:0003724 RNA helicase activity
• GO:0046332 SMAD binding
• GO:0000380 alternative mRNA splicing, via spliceosome
• GO:0030521 androgen receptor signaling pathway
• GO:0030509 BMP signaling pathway
• GO:0001837 epithelial to mesenchymal transition
• GO:0030520 estrogen receptor signaling pathway
• GO:0072332 intrinsic apoptotic signaling pathway by p53 class mediator
• GO:0061614 miRNA transcription
• GO:0000398 mRNA splicing, via spliceosome
• GO:0009299 mRNA transcription
• GO:0045445 myoblast differentiation
• GO:0000122 negative regulation of transcription by RNA polymerase II
• GO:0000956 nuclear-transcribed mRNA catabolic process
• GO:0043517 positive regulation of DNA damage response, signal transduction by p53 class mediator
• GO:0031053 primary miRNA processing
• GO:0000381 regulation of alternative mRNA splicing, via spliceosome
• GO:0060765 regulation of androgen receptor signaling pathway
• GO:0045667 regulation of osteoblast differentiation
• GO:2001014 regulation of skeletal muscle cell differentiation
• GO:0006357 regulation of transcription by RNA polymerase II
• GO:0045069 regulation of viral genome replication
• GO:0048511 rhythmic process

Neighborhood · nearest proteins

Related proteins · sequence + function similarity

• Probable ATP-dependent RNA helicase DDX5 1.00
• Probable ATP-dependent RNA helicase DDX5 1.00
• Probable ATP-dependent RNA helicase DDX5 1.00
• Probable ATP-dependent RNA helicase DDX5 1.00
• Probable ATP-dependent RNA helicase DDX17 0.94
• ATP-dependent RNA helicase dbp2 0.92
• ATP-dependent RNA helicase DBP2 0.92
• ATP-dependent RNA helicase dbp2 0.91
• DEAD-box ATP-dependent RNA helicase 30 0.91
• ATP-dependent RNA helicase dbp2 0.91
• ATP-dependent RNA helicase dbp2 0.91
• ATP-dependent RNA helicase DBP2 0.90

Co-cited proteins · studied together in the literature

• Probable ATP-dependent RNA helicase DDX17 10 shared papers
• ATP-dependent RNA helicase dbp2 1 shared papers
• Probable ATP-dependent RNA helicase DDX5 2 shared papers
• Estrogen receptor 3 shared papers
• RNA-binding protein 4 2 shared papers
• rRNA 2'-O-methyltransferase fibrillarin 1 shared papers
• ATP-dependent RNA helicase DBP2 1 shared papers
• Probable ATP-dependent RNA helicase DDX17 1 shared papers
• Heterogeneous nuclear ribonucleoprotein H2 1 shared papers
• ATP-dependent RNA helicase DDX3X 2 shared papers
• Runt-related transcription factor 2 1 shared papers
• Splicing regulator ARVCF 1 shared papers

Literature · 45 cited papers

• Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation. Nat. Struct. Mol. Biol. · 2017
• Identification of Novel Proteins Co-Purifying with Cockayne Syndrome Group B (CSB) Reveals Potential Roles for CSB in RNA Metabolism and Chromatin Dynamics. PLoS ONE · 2015
• N-terminome analysis of the human mitochondrial proteome. Proteomics · 2015
• SUMO-2 orchestrates chromatin modifiers in response to DNA damage. Cell Rep. · 2015
• System-wide analysis of SUMOylation dynamics in response to replication stress reveals novel small ubiquitin-like modified target proteins and acceptor lysines relevant for genome stability. Mol. Cell. Proteomics · 2015
• PMID 25452582 EMBO J. · 2014
• Uncovering global SUMOylation signaling networks in a site-specific manner. Nat. Struct. Mol. Biol. · 2014
• Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli. Proc. Natl. Acad. Sci. U.S.A. · 2014
• Nuclear ARVCF protein binds splicing factors and contributes to the regulation of alternative splicing. J. Biol. Chem. · 2014
• PMID 24509260 Mol. Cell. Biol. · 2014
• Toward a comprehensive characterization of a human cancer cell phosphoproteome. J. Proteome Res. · 2013
• The DEAD-box RNA helicase DDX3 interacts with DDX5, co-localizes with it in the cytoplasm during the G2/M phase of the cycle, and affects its shuttling during mRNP export. J. Cell. Biochem. · 2012
• … and 33 more in the literature graph