Polyadenylate-binding protein 1

Function

Binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Binds to N6-methyladenosine (m6A)-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability.

Classification

Family (Pfam)

PF00658 MLLE, PF00076 RRM_1

InterPro

Nucleotide-bd_a/b_plait_sf, PABP-dom, PABP_1234, PABP_HYD_C, PABP_RRM1, RBD_domain_sf, RRM2_I_PABPs, RRM_dom, RRM_euk-type

Functional cluster

RNA-Polymerase II Transcription Factors

Experimental structures · PDB · 29

• 1CVJ X-ray 2.60A
• 1G9L NMR
• 1JGN NMR
• 1JH4 NMR
• 2K8G NMR
• 2RQG NMR
• 2RQH NMR
• 2X04 X-ray 1.49A
• 3KTP X-ray 1.50A
• 3KTR X-ray 1.70A
• 3KUI X-ray 2.30A
• 3KUJ X-ray 1.40A
• … and 17 more

A predicted model is available from AlphaFold.

Gene Ontology · 29

• GO:0071013 catalytic step 2 spliceosome
• GO:0031252 cell leading edge
• GO:0005737 cytoplasm
• GO:0036464 cytoplasmic ribonucleoprotein granule
• GO:0010494 cytoplasmic stress granule
• GO:0005829 cytosol
• GO:0070062 extracellular exosome
• GO:0005925 focal adhesion
• GO:0030027 lamellipodium
• GO:0016020 membrane
• GO:0005634 nucleus
• GO:1990904 ribonucleoprotein complex
• GO:0003730 mRNA 3'-UTR binding
• GO:0003729 mRNA binding
• GO:0008143 poly(A) binding
• GO:0008266 poly(U) RNA binding
• GO:0003723 RNA binding
• GO:0008494 translation activator activity
• GO:0070934 CRD-mediated mRNA stabilization
• GO:0000398 mRNA splicing, via spliceosome
• GO:0048255 mRNA stabilization
• GO:1900152 negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
• GO:2000623 negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
• GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
• GO:2000767 positive regulation of cytoplasmic translation
• GO:1900153 positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
• GO:0060213 positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
• GO:0045070 positive regulation of viral genome replication
• GO:0031047 regulatory ncRNA-mediated gene silencing

Neighborhood · nearest proteins

Related proteins · sequence + function similarity

• Polyadenylate-binding protein 1 1.00
• Polyadenylate-binding protein 1 1.00
• Polyadenylate-binding protein 1 1.00
• Polyadenylate-binding protein 1-B 0.99
• Polyadenylate-binding protein 1-A 0.99
• Polyadenylate-binding protein 1A 0.99
• Polyadenylate-binding protein 3 0.97
• Embryonic polyadenylate-binding protein 0.95
• Embryonic polyadenylate-binding protein A 0.95
• Polyadenylate-binding protein 4 0.95
• Embryonic polyadenylate-binding protein B 0.95
• Polyadenylate-binding protein 0.94

Co-cited proteins · studied together in the literature

• Polyadenylate-binding protein-interacting protein 2 4 shared papers
• Polyadenylate-binding protein-interacting protein 1 5 shared papers
• La-related protein 1 4 shared papers
• Endonuclease V 1 shared papers
• La-related protein 4B 1 shared papers
• Insulin-like growth factor 2 mRNA-binding protein 1 7 shared papers
• Post-transcriptional regulator MTA 1 shared papers
• Cold shock domain-containing protein E1 3 shared papers
• ATP-dependent RNA helicase with zinc finger domain 1 shared papers
• ATP-dependent RNA helicase with zinc finger domain 2 shared papers
• Eukaryotic initiation factor 4A-I 3 shared papers
• Polyadenylate-binding protein 4 3 shared papers

Literature · 63 cited papers

• The SARS-unique domain (SUD) of SARS-CoV and SARS-CoV-2 interacts with human Paip1 to enhance viral RNA translation. EMBO J. · 2021
• An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis. Nat. Commun. · 2020
• The Interactome analysis of the Respiratory Syncytial Virus protein M2-1 suggests a new role in viral mRNA metabolism post-transcription. Sci. Rep. · 2019
• HELZ directly interacts with CCR4-NOT and causes decay of bound mRNAs. Life. Sci Alliance · 2019
• Recognition of RNA N6-methyladenosine by IGF2BP proteins enhances mRNA stability and translation. Nat. Cell Biol. · 2018
• The non-canonical poly(A) polymerase FAM46C acts as an onco-suppressor in multiple myeloma. Nat. Commun. · 2017
• The helicase, DDX3X, interacts with poly(A)-binding protein 1 (PABP1) and caprin-1 at the leading edge of migrating fibroblasts and is required for efficient cell spreading. Biochem. J. · 2017
• Antagonistic actions of two human Pan3 isoforms on global mRNA turnover. RNA · 2017
• Identification of a nuclear exosome decay pathway for processed transcripts. Mol. Cell · 2016
• Regulation of Human Endonuclease V Activity and Relocalization to Cytoplasmic Stress Granules. J. Biol. Chem. · 2016
• Characterization of RyDEN (C19orf66) as an interferon-stimulated cellular inhibitor against dengue virus replication. PLoS Pathog. · 2016
• N-terminome analysis of the human mitochondrial proteome. Proteomics · 2015
• … and 51 more in the literature graph