D-alanyl-D-alanine carboxypeptidase DacA

UniProt P0AEB2 Organism Escherichia coli (strain K12) Gene JW0627, b0632, dacA, pfv EC 3.4.16.4, 3.5.2.6

Also known as: JW0627, b0632, dacA, pfv

Function

Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.

Classification

Family (Pfam): PF07943 PBP5_C, PF00768 Peptidase_S11
InterPro: Beta-lactam/transpept-like, Peniciliin-bd_prot_C_sf, Peptidase_S11, Peptidase_S11_C, Peptidase_S11_C_sf, Peptidase_S11_N
Functional cluster: Membrane Insertases & Cytochromes

Experimental structures · PDB · 13

1HD8 X-ray 2.30A
1NJ4 X-ray 1.90A
1NZO X-ray 1.85A
1NZU X-ray 2.00A
1SDN X-ray 2.50A
1Z6F X-ray 1.60A
3BEB X-ray 2.00A
3BEC X-ray 1.60A
3MZD X-ray 1.90A
3MZE X-ray 2.10A
3MZF X-ray 1.50A
5J8X X-ray 2.53A
… and 1 more

A predicted model is available from AlphaFold.

Gene Ontology · 13

Drugs targeting this protein · 85

CEFOTAXIME SODIUM inhibitor
AMOXICILLIN inhibitor
CEFUROXIME AXETIL inhibitor
PENICILLIN G SODIUM inhibitor
CEFAMANDOLE inhibitor
CYCLACILLIN inhibitor
MOXALACTAM DISODIUM inhibitor
CEPHALEXIN HYDROCHLORIDE inhibitor
CEFOPERAZONE SODIUM inhibitor
CEFAZOLIN SODIUM inhibitor
CEFOXITIN SODIUM inhibitor
CEPHALEXIN inhibitor
LORACARBEF inhibitor
CEFPIRAMIDE SODIUM inhibitor
AMPICILLIN SODIUM inhibitor
CEFTAZIDIME SODIUM inhibitor
PIPERACILLIN SODIUM inhibitor
PENICILLIN V POTASSIUM inhibitor
TICARCILLIN DISODIUM inhibitor
CEFEPIME HYDROCHLORIDE inhibitor
BACAMPICILLIN HYDROCHLORIDE inhibitor
CEPHALOGLYCIN inhibitor
CEFTIBUTEN DIHYDRATE inhibitor
CARBENICILLIN INDANYL SODIUM inhibitor
CEFONICID SODIUM inhibitor
CEFPODOXIME PROXETIL inhibitor
CEFACLOR inhibitor
CEFTIZOXIME SODIUM inhibitor
CEPHAPIRIN SODIUM inhibitor
CEFORANIDE inhibitor
CEFOTETAN DISODIUM inhibitor
CEFMETAZOLE SODIUM inhibitor
CEFTOLOZANE SULFATE inhibitor
PENICILLIN G POTASSIUM inhibitor
ERTAPENEM SODIUM inhibitor
RAZUPENEM inhibitor
FAROPENEM MEDOXOMIL inhibitor
MEROPENEM inhibitor
TEMOCILLIN inhibitor
ERTAPENEM inhibitor
CEFIXIME inhibitor
FLOMOXEF inhibitor
AZTREONAM inhibitor
CEPHRADINE inhibitor
CEFTIBUTEN inhibitor
PHENETHICILLIN inhibitor
CEFAMANDOLE NAFATE inhibitor
TALAMPICILLIN inhibitor
CEPHALOTHIN SODIUM inhibitor
CEFADROXIL inhibitor
CEFPODOXIME inhibitor
MEZLOCILLIN SODIUM inhibitor
AMPICILLIN inhibitor
SULOPENEM inhibitor
PENAMECILLIN inhibitor
TEBIPENEM PIVOXIL inhibitor
CEFUROXIME SODIUM inhibitor
FLOXACILLIN inhibitor
CEFTIOFUR inhibitor
CEFODIZIME inhibitor
PIVAMPICILLIN inhibitor
CEFMENOXIME HYDROCHLORIDE inhibitor
CEFSULODIN inhibitor
CEFTAROLINE FOSAMIL ACETATE inhibitor
AZTREONAM LYSINE inhibitor
PENICILLIN G BENZATHINE inhibitor
CEFIDEROCOL inhibitor
CEFIDEROCOL SULFATE TOSYLATE inhibitor
CEFILAVANCIN inhibitor
CEFOTIAM HYDROCHLORIDE inhibitor
DORIPENEM MONOHYDRATE inhibitor
IMIPENEM inhibitor
CEFTAZIDIME inhibitor
CEFDITOREN PIVOXIL inhibitor
SULOPENEM ETZADROXIL inhibitor
DORIPENEM inhibitor
CEFTAROLINE FOSAMIL inhibitor
CEFTOBIPROLE inhibitor
CEFPROZIL, (E)- inhibitor
FAROPENEM inhibitor
CARBENICILLIN DISODIUM inhibitor
CEFTRIAXONE SODIUM inhibitor
PENICILLIN V inhibitor
CEFPIROME inhibitor
CEFDINIR inhibitor

Related proteins · sequence + function similarity

D-alanyl-D-alanine carboxypeptidase DacA 1.00
D-alanyl-D-alanine carboxypeptidase DacA 1.00
D-alanyl-D-alanine carboxypeptidase DacC 0.92
D-alanyl-D-alanine carboxypeptidase DacA 0.90
D-alanyl-D-alanine carboxypeptidase DacD 0.86
D-alanyl-D-alanine carboxypeptidase DacD 0.82
D-alanyl-D-alanine endopeptidase 0.76
D-alanyl-D-alanine endopeptidase 0.76
D-alanyl-D-alanine endopeptidase 0.72
Beta-lactamase SME-1 0.70
Beta-lactamase OXY-2 0.69
Beta-lactamase OXY-1 0.69

Co-cited proteins · studied together in the literature

D-alanyl-D-alanine carboxypeptidase DacC 2 shared papers
Endolytic peptidoglycan transglycosylase RlpA 1 shared papers
Peptidoglycan glycosyltransferase MrdB 1 shared papers
LPS-assembly lipoprotein LptE 1 shared papers
DNA polymerase III subunit delta 1 shared papers

Literature · 13 cited papers

Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol. Syst. Biol. · 2006
Crystal structure of Escherichia coli penicillin-binding protein 5 bound to a tripeptide boronic acid inhibitor: a role for Ser-110 in deacylation. Biochemistry · 2005
Crystal structure of wild-type penicillin-binding protein 5 from Escherichia coli. Implications for deacylation of the acyl-enzyme complex. J. Biol. Chem. · 2003
Crystal structure of a deacylation-defective mutant of penicillin-binding protein 5 at 2.3-A resolution. J. Biol. Chem. · 2001
Escherichia coli proteome analysis using the gene-protein database. Electrophoresis · 1997
The complete genome sequence of Escherichia coli K-12. Science · 1997
A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. · 1996
Domain organization of penicillin-binding protein 5 from Escherichia coli analysed by C-terminal truncation. Biochem. J. · 1993
Amino acid sequence homologies between Escherichia coli penicillin-binding protein 5 and class A beta-lactamases. FEBS Lett. · 1982
An amino acid substitution that blocks the deacylation step in the enzyme mechanism of penicillin-binding protein 5 of Escherichia coli. FEBS Lett. · 1984
Genes encoding two lipoproteins in the leuS-dacA region of the Escherichia coli chromosome. J. Bacteriol. · 1987
Nucleotide sequences of the penicillin-binding protein 5 and 6 genes of Escherichia coli. Nucleic Acids Res. · 1988
… and 1 more in the literature graph

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