Nucleoprotein

Function

Encapsidates the viral RNA genome by forming a left-handed helical nucleocapsid that protects the RNA from nucleases. RNA replication depends on the availability of soluble nucleoprotein. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Together with the phosphoprotein, sequesters host NF-kappa-B in inclusion bodies (IBs) thereby inhibiting this host defense pathway. May also act as a modulator of the innate immune response by sequestration of host IFIH1/MDA5 and MAVS into IBs.

Classification

Family (Pfam)

PF03246 Pneumo_ncap

InterPro

Pneumo_ncap

Functional cluster

Viral Structural & Replication Proteins

Experimental structures · PDB · 16

• 2WJ8 X-ray 3.29A
• 2YHM X-ray 3.60A
• 4BKK EM
• 4UC6 X-ray 2.10A
• 4UC7 X-ray 2.45A
• 4UC8 X-ray 2.00A
• 4UC9 X-ray 2.40A
• 4UCA X-ray 3.22A
• 4UCB X-ray 2.79A
• 4UCC X-ray 2.05A
• 4UCD X-ray 2.66A
• 4UCE X-ray 2.95A
• … and 4 more

A predicted model is available from AlphaFold.

Gene Ontology · 12

• GO:0019029 helical viral capsid
• GO:0030430 host cell cytoplasm
• GO:1990904 ribonucleoprotein complex
• GO:0019028 viral capsid
• GO:0019013 viral nucleocapsid
• GO:0030291 protein serine/threonine kinase inhibitor activity
• GO:0003723 RNA binding
• GO:0039545 symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity
• GO:0039554 symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MDA-5 activity
• GO:0085034 symbiont-mediated suppression of host NF-kappaB cascade
• GO:0039580 symbiont-mediated suppression of host PKR/eIFalpha signaling
• GO:0039502 symbiont-mediated suppression of host type I interferon-mediated signaling pathway

Drugs targeting this protein · 1

• RSV-604 inhibitor

Related proteins · sequence + function similarity

• Nucleoprotein 0.98
• Nucleoprotein 0.98
• Nucleoprotein 0.98
• Nucleoprotein 0.98
• Nucleoprotein 0.98
• Nucleoprotein 0.97
• Methyl-coenzyme M reductase subunit beta 0.81
• Methyl-coenzyme M reductase subunit alpha 0.80
• Methyl-coenzyme M reductase subunit beta 0.80
• Methyl-coenzyme M reductase I subunit alpha 0.78
• Fructose-1,6-bisphosphate aldolase/phosphatase 0.77
• Methyl-coenzyme M reductase subunit alpha 0.77

Co-cited proteins · studied together in the literature

• Phosphoprotein 14 shared papers
• Nucleoprotein 1 shared papers
• RNA-directed RNA polymerase L 5 shared papers
• Protein M2-2 5 shared papers
• Eukaryotic translation initiation factor 1A, X-chromosomal 1 shared papers
• Protein M2-1 4 shared papers
• Phosphoprotein 1 shared papers
• Nucleoprotein 1 shared papers
• Tax1-binding protein 1 1 shared papers
• Fusion glycoprotein F0 4 shared papers
• Interferon-induced, double-stranded RNA-activated protein kinase 1 shared papers
• Major surface glycoprotein G 3 shared papers

Literature · 29 cited papers

• Depletion of TAX1BP1 Amplifies Innate Immune Responses during Respiratory Syncytial Virus Infection. J. Virol. · 2021
• Respiratory syncytial virus sequesters NF-kappaB subunit p65 to cytoplasmic inclusion bodies to inhibit innate immune signalling. J. Virol. · 2020
• A conformational switch balances viral RNA accessibility and protection in a nucleocapsid ring model. Arch. Biochem. Biophys. · 2019
• Biochemical characterization of the respiratory syncytial virus N0-P complex in solution. J. Biol. Chem. · 2019
• New Insights into Structural Disorder in Human Respiratory Syncytial Virus Phosphoprotein and Implications for Binding of Protein Partners. J. Biol. Chem. · 2017
• Phosphorylation of the human respiratory syncytial virus P protein mediates M2-2 regulation of viral RNA synthesis, a process that involves two P proteins. Virus Res. · 2016
• A Druggable Pocket at the Nucleocapsid/Phosphoprotein Interaction Site of Human Respiratory Syncytial Virus. J. Virol. · 2015
• Identification and characterization of the binding site of the respiratory syncytial virus phosphoprotein to RNA-free nucleoprotein. J. Virol. · 2015
• The eukaryotic elongation factor 1A is critical for genome replication of the paramyxovirus respiratory syncytial virus. PLoS ONE · 2014
• Quantitative investigation of the affinity of human respiratory syncytial virus phosphoprotein C-terminus binding to nucleocapsid protein. Virol. J. · 2014
• The respiratory syncytial virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid. J. Gen. Virol. · 2013
• Characterization of a viral phosphoprotein binding site on the surface of the respiratory syncytial nucleoprotein. J. Virol. · 2012
• … and 17 more in the literature graph