Isoleucine--tRNA ligase

UniProt Q99YW3 Organism Streptococcus pyogenes serotype M1 Gene M5005_Spy1243, SPy_1513, ileS EC 6.1.1.5

Also known as: M5005_Spy1243, SPy_1513, ileS

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Classification

Family (Pfam): PF08264 Anticodon_1, PF00133 tRNA-synt_1
InterPro: aa-tRNA-synth_I_CS, aa-tRNA-synth_Ia, Anticodon_Ile_BEm, Ile-tRNA-ligase, Ile-tRNA-ligase_type1, Ile-tRNA_ligase, M/V/L/I-tRNA-synth_anticd-bd, Rossmann-like_a/b/a_fold, tRNAsynth_Ia_anticodon-bd, Val/Leu/Ile-tRNA-synth_edit
Functional cluster: Mur-Ligase Peptidoglycan Biosynthesis Enzymes

Gene Ontology · 6

Drugs targeting this protein · 2

MUPIROCIN CALCIUM inhibitor
MUPIROCIN inhibitor

Related proteins · sequence + function similarity

Literature · 2 cited papers

Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events. J. Infect. Dis. · 2005
Complete genome sequence of an M1 strain of Streptococcus pyogenes. Proc. Natl. Acad. Sci. U.S.A. · 2001

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