ATP-dependent RNA helicase DDX1

UniProt Q92499 Organism Homo sapiens Gene DDX1 EC 3.6.4.13

Also known as: DDX1

Function

Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. It is also an accessory subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. Cooperates with ZBTB8OS (also known as archease) for the guanylylation of RTCB, a key intermediate step in activation of the tRNA ligase. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines via the adapter molecule TICAM1. Specifically binds (via helicase ATP-binding domain) on both short and long poly(I:C) dsRNA (By similarity).

Classification

Family (Pfam): PF00270 DEAD, PF00271 Helicase_C, PF00622 SPRY
InterPro: B30.2/SPRY, B30.2/SPRY_sf, ConA-like_dom_sf, DEAD/DEAH_box_helicase_dom, Helicase_ATP-bd, Helicase_C-like, P-loop_NTPase, RNA_helicase_DEAD_Q_motif, SPRY_dom
Functional cluster: Serine/Threonine Protein Kinases

Experimental structures · PDB · 2

4XW3 X-ray 2.00A
8TBX X-ray 2.71A

A predicted model is available from AlphaFold.

Gene Ontology · 32

Neighborhood · nearest proteins

Nearest neighbours of ATP-dependent RNA helicase DDX1: 3 by sequence/function similarity (left); 3 co-cited in the literature (right).

Related proteins · sequence + function similarity

Co-cited proteins · studied together in the literature

Protein Rev 1 shared papers
tRNA-splicing ligase complex subunit FAM98B 4 shared papers
tRNA-splicing ligase complex subunit RTRAF 4 shared papers
Cleavage stimulation factor subunit 2 1 shared papers
tRNA-splicing ligase complex subunit ASW 2 shared papers
Heterogeneous nuclear ribonucleoprotein K 1 shared papers
Protein FAM98A 1 shared papers
RNA-splicing ligase RTCB 3 shared papers
ATP-dependent RNA helicase DDX3X 3 shared papers
Polyprotein P1234 1 shared papers
Muscleblind-like protein 1 1 shared papers
Serine-protein kinase ATM 1 shared papers

Literature · 28 cited papers

Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation. Nat. Struct. Mol. Biol. · 2017
FAM98A associates with DDX1-C14orf166-FAM98B in a novel complex involved in colorectal cancer progression. Int. J. Biochem. Cell Biol. · 2017
Venezuelan equine encephalitis virus non-structural protein 3 (nsP3) interacts with RNA helicases DDX1 and DDX3 in infected cells. Antiviral Res. · 2016
Structure of the SPRY domain of the human RNA helicase DDX1, a putative interaction platform within a DEAD-box protein. Acta Crystallogr. F · 2015
Identification of Novel Proteins Co-Purifying with Cockayne Syndrome Group B (CSB) Reveals Potential Roles for CSB in RNA Metabolism and Chromatin Dynamics. PLoS ONE · 2015
N-terminome analysis of the human mitochondrial proteome. Proteomics · 2015
Analysis of orthologous groups reveals archease and DDX1 as tRNA splicing factors. Nature · 2014
hCLE/C14orf166 associates with DDX1-HSPC117-FAM98B in a novel transcription-dependent shuttling RNA-transporting complex. PLoS ONE · 2014
An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
Toward a comprehensive characterization of a human cancer cell phosphoproteome. J. Proteome Res. · 2013
The X-chromosome-linked intellectual disability protein PQBP1 is a component of neuronal RNA granules and regulates the appearance of stress granules. Hum. Mol. Genet. · 2011
A motif unique to the human DEAD-box protein DDX3 is important for nucleic acid binding, ATP hydrolysis, RNA/DNA unwinding and HIV-1 replication. PLoS ONE · 2011
… and 16 more in the literature graph

A document in the lmmol reference corpus — open public data (UniProt, GO, PDB, the literature graph) rendered as a single cross-linked page. Hover any link to preview its target.