Cyclin-dependent kinase 13

Function

Cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, probably by phosphorylating SRSF1/SF2. Required during hematopoiesis. In case of infection by HIV-1 virus, interacts with HIV-1 Tat protein acetylated at 'Lys-50' and 'Lys-51', thereby increasing HIV-1 mRNA splicing and promoting the production of the doubly spliced HIV-1 protein Nef.

Classification

Family (Pfam)

PF00069 Pkinase

InterPro

CDK, Kinase-like_dom_sf, Prot_kinase_dom, Protein_kinase_ATP_BS, Ser/Thr_kinase_AS

Functional cluster

RRM RNA-Binding & Protein Kinases

Experimental structures · PDB · 2

• 5EFQ X-ray 2.00A
• 7NXJ X-ray 2.36A

A predicted model is available from AlphaFold.

Gene Ontology · 22

• GO:0002945 cyclin K-CDK13 complex
• GO:0008024 cyclin/CDK positive transcription elongation factor complex
• GO:0005576 extracellular region
• GO:0005615 extracellular space
• GO:1904813 ficolin-1-rich granule lumen
• GO:0019908 nuclear cyclin-dependent protein kinase holoenzyme complex
• GO:0016607 nuclear speck
• GO:0005654 nucleoplasm
• GO:0005634 nucleus
• GO:0005524 ATP binding
• GO:0030332 cyclin binding
• GO:0004693 cyclin-dependent protein serine/threonine kinase activity
• GO:0004672 protein kinase activity
• GO:0019901 protein kinase binding
• GO:0106310 protein serine kinase activity
• GO:0003723 RNA binding
• GO:0008353 RNA polymerase II CTD heptapeptide repeat kinase activity
• GO:0000380 alternative mRNA splicing, via spliceosome
• GO:0030097 hemopoiesis
• GO:0045944 positive regulation of transcription by RNA polymerase II
• GO:0032968 positive regulation of transcription elongation by RNA polymerase II
• GO:0009966 regulation of signal transduction

Disease associations

• congenital heart defects, dysmorphic facial features, and intellectual developmental disorder MONDO:0044302

Drugs targeting this protein · 4

• PHA-793887 inhibitor
• RONICICLIB inhibitor
• AT-7519 inhibitor
• AZD-5438 inhibitor

Related proteins · sequence + function similarity

• Cyclin-dependent kinase 13 1.00
• Cyclin-dependent kinase 13 0.99
• Cyclin-dependent kinase 12 0.83
• Cyclin-dependent kinase 12 0.83
• Cyclin-dependent kinase 12 0.82
• Cyclin-dependent kinase 12 0.82
• Cyclin-dependent kinase 12 0.82
• Cyclin-dependent kinase 12 0.81
• Cyclin-dependent kinase 12 0.78
• Zinc finger CCCH domain-containing protein 18 0.76
• Zinc finger CCCH domain-containing protein 18 0.76
• Zinc finger CCCH domain-containing protein 18 0.75

Co-cited proteins · studied together in the literature

• Coatomer subunit alpha 1 shared papers
• Cyclin-dependent kinase 12 1 shared papers
• Cyclin-dependent kinase 12 3 shared papers
• Protein Tat 1 shared papers
• Cyclin-dependent kinase 12 1 shared papers
• Complement component 1 Q subcomponent-binding protein, mitochondrial 1 shared papers
• Serine/threonine-protein kinase D1 1 shared papers
• Cyclin-dependent kinase 13 1 shared papers
• Cyclin-K 1 shared papers
• Cyclin-K 2 shared papers
• ATP-dependent chromatin remodeler CHD4 1 shared papers
• Cyclin-L1 1 shared papers

Literature · 29 cited papers

• HSV-1 ICP22 Is a Selective Viral Repressor of Cellular RNA Polymerase II-Mediated Transcription Elongation. Vaccines (Basel) · 2021
• Phenotypic and molecular characterisation of CDK13-related congenital heart defects, dysmorphic facial features and intellectual developmental disorders. Genome Med. · 2017
• Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation. Nat. Struct. Mol. Biol. · 2017
• Distinct genetic architectures for syndromic and nonsyndromic congenital heart defects identified by exome sequencing. Nat. Genet. · 2016
• An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
• Toward a comprehensive characterization of a human cancer cell phosphoproteome. J. Proteome Res. · 2013
• The Cyclin K/Cdk12 complex maintains genomic stability via regulation of expression of DNA damage response genes. Genes Dev. · 2011
• Genome-wide evaluation and discovery of vertebrate A-to-I RNA editing sites. Biochem. Biophys. Res. Commun. · 2011
• System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci. Signal. · 2011
• Initial characterization of the human central proteome. BMC Syst. Biol. · 2011
• CDK12 is a transcription elongation-associated CTD kinase, the metazoan ortholog of yeast Ctk1. Genes Dev. · 2010
• Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci. Signal. · 2010
• … and 17 more in the literature graph