Serine/arginine-rich splicing factor 1

UniProt Q07955 Organism Homo sapiens Gene ASF, SF2, SF2P33, SFRS1, SRSF1

Also known as: ASF, SF2, SF2P33, SFRS1, SRSF1

Function

Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.

Classification

Family (Pfam): PF00076 RRM_1
InterPro: Nucleotide-bd_a/b_plait_sf, RBD_domain_sf, RRM_dom, RRT5_SRSF_SR, SRSF1_RRM1, SRSF1_RRM2
Functional cluster: RNA-Binding & Splicing Proteins

Experimental structures · PDB · 9

1X4A NMR
2M7S NMR
2M8D NMR
2O3D NMR
3BEG X-ray 2.90A
4C0O X-ray 2.56A
6HPJ NMR
7ABG EM 7.80A
8QO9 EM 5.29A

A predicted model is available from AlphaFold.

Gene Ontology · 22

Disease associations

neurodevelopmental disorder with dysmorphic facies and behavioral abnormalities MONDO:0957583

Neighborhood · nearest proteins

Nearest neighbours of Serine/arginine-rich splicing factor 1: 3 by sequence/function similarity (left); 3 co-cited in the literature (right).

Related proteins · sequence + function similarity

Co-cited proteins · studied together in the literature

SRSF protein kinase 1 7 shared papers
Serine/arginine-rich splicing factor 2 7 shared papers
Serine-arginine protein 55 1 shared papers
Constitutive coactivator of PPAR-gamma-like protein 1 1 shared papers
Nuclear speckle splicing regulatory protein 1 1 shared papers
Nuclear speckle splicing regulatory protein 1 1 shared papers
PC4 and SFRS1-interacting protein 1 shared papers
Serine/arginine-rich splicing factor 3 3 shared papers
Luc7-like protein 3 2 shared papers
Serine/Arginine-related protein 53 1 shared papers
Serine/Arginine-related protein 53 1 shared papers
Splicing regulatory glutamine/lysine-rich protein 1 1 shared papers

Literature · 48 cited papers

FAM120A couples SREBP-dependent transcription and splicing of lipogenesis enzymes downstream of mTORC1. Mol. Cell · 2023
SRSF1 haploinsufficiency is responsible for a syndromic developmental disorder associated with intellectual disability. Am. J. Hum. Genet. · 2023
Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation. Nat. Struct. Mol. Biol. · 2017
N-terminome analysis of the human mitochondrial proteome. Proteomics · 2015
System-wide analysis of SUMOylation dynamics in response to replication stress reveals novel small ubiquitin-like modified target proteins and acceptor lysines relevant for genome stability. Mol. Cell. Proteomics · 2015
Nuclear ARVCF protein binds splicing factors and contributes to the regulation of alternative splicing. J. Biol. Chem. · 2014
Structural basis for nuclear import of splicing factors by human Transportin 3. Proc. Natl. Acad. Sci. U.S.A. · 2014
An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
Immunoaffinity enrichment and mass spectrometry analysis of protein methylation. Mol. Cell. Proteomics · 2014
RRP1B is a metastasis modifier that regulates the expression of alternative mRNA isoforms through interactions with SRSF1. Oncogene · 2014
Toward a comprehensive characterization of a human cancer cell phosphoproteome. J. Proteome Res. · 2013
System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci. Signal. · 2011
… and 36 more in the literature graph

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