Elongation factor 1-alpha 2

Function

Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome (By similarity). The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation (By similarity).

Classification

Family (Pfam)

PF22594 GTP-eEF1A_C, PF00009 GTP_EFTU, PF03144 GTP_EFTU_D2

InterPro

EFTu-like_2, G_TR_CS, GTP-eEF1A_C, P-loop_NTPase, T_Tr_GTP-bd_dom, TRAFAC_GTPase_members, Transl_B-barrel_sf, Transl_elong_EF1A/Init_IF2_C, Transl_elong_EF1A_euk/arc

Functional cluster

Translational GTPases (EF-Tu/EF-G)

Experimental structures · PDB · 2

• 3C5J X-ray 1.80A
• 8B6Z EM 2.90A

A predicted model is available from AlphaFold.

Gene Ontology · 15

• GO:0005737 cytoplasm
• GO:0098574 cytoplasmic side of lysosomal membrane
• GO:0005789 endoplasmic reticulum membrane
• GO:0005853 eukaryotic translation elongation factor 1 complex
• GO:0045202 synapse
• GO:0005525 GTP binding
• GO:0003924 GTPase activity
• GO:0046872 metal ion binding
• GO:0019901 protein kinase binding
• GO:0003746 translation elongation factor activity
• GO:0008135 translation factor activity, RNA binding
• GO:0090218 positive regulation of lipid kinase activity
• GO:1904714 regulation of chaperone-mediated autophagy
• GO:0006412 translation
• GO:0006414 translational elongation

Disease associations

• intellectual disability, autosomal dominant 38 MONDO:0014617
• developmental and epileptic encephalopathy, 33 MONDO:0014625

Drugs targeting this protein · 1

• PLITIDEPSIN inhibitor

Related proteins · sequence + function similarity

• Elongation factor 1-alpha 2 1.00
• Elongation factor 1-alpha 2 1.00
• Elongation factor 1-alpha 2 1.00
• Elongation factor 1-alpha 1 0.98
• Elongation factor 1-alpha, somatic form 0.98
• Elongation factor 1-alpha 1 0.98
• Elongation factor 1-alpha 1 0.98
• Elongation factor 1-alpha 1 0.98
• Elongation factor 1-alpha 1 0.98
• Elongation factor 1-alpha 1 0.98
• Elongation factor 1-alpha 1 0.98
• Elongation factor 1-alpha 1 0.98

Co-cited proteins · studied together in the literature

• Elongation factor 1-alpha 1 2 shared papers
• eEF1A lysine and N-terminal methyltransferase 2 shared papers
• EEF1A lysine methyltransferase 4 1 shared papers
• Elongation factor 1-alpha 2 1 shared papers
• Elongation factor 1-alpha 1 4 shared papers
• Elongation factor 1-alpha 1 1 shared papers
• EEF1A lysine methyltransferase 3 1 shared papers
• Elongation factor 1-alpha 2 shared papers
• EEF1A lysine methyltransferase 3 1 shared papers
• Olfactory receptor 10H2 1 shared papers
• Probable tRNA methyltransferase 9B 1 shared papers
• HLA class II histocompatibility antigen, DR beta 3 chain 1 shared papers

Literature · 21 cited papers

• Visualization of translation and protein biogenesis at the ER membrane. Nature · 2023
• METTL13 methylation of eEF1A increases translational output to promote tumorigenesis. Cell · 2018
• The dual methyltransferase METTL13 targets N terminus and Lys55 of eEF1A and modulates codon-specific translation rates. Nat. Commun. · 2018
• Methylation of human eukaryotic elongation factor alpha (eEF1A) by a member of a novel protein lysine methyltransferase family modulates mRNA translation. Nucleic Acids Res. · 2017
• The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A). Nucleic Acids Res. · 2017
• Identification of novel genetic causes of Rett syndrome-like phenotypes. J. Med. Genet. · 2016
• PMID 26330559 FASEB J. · 2015
• De novo EEF1A2 mutations in patients with characteristic facial features, intellectual disability, autistic behaviors and epilepsy. Clin. Genet. · 2015
• Immunoaffinity enrichment and mass spectrometry analysis of protein methylation. Mol. Cell. Proteomics · 2014
• Exome sequencing reveals new causal mutations in children with epileptic encephalopathies. Epilepsia · 2013
• Diagnostic exome sequencing in persons with severe intellectual disability. N. Engl. J. Med. · 2012
• Initial characterization of the human central proteome. BMC Syst. Biol. · 2011
• … and 9 more in the literature graph