D-alanine--D-alanine ligase

Function

Catalyzes the ATP-driven ligation of two D-alanine molecules to form the D-alanyl-D-alanine dipeptide. This molecule is a key building block in peptidoglycan biosynthesis.

Classification

Family (Pfam)

PF07478 Dala_Dala_lig_C, PF01820 Dala_Dala_lig_N

InterPro

ATP-grasp, ATP_grasp_subdomain_1, D-Ala_lig_Van_CS, D_ala_D_ala, Dala_Dala_lig_C, Dala_Dala_lig_N, PreATP-grasp_dom_sf

Functional cluster

Acyltransferases & Methyltransferases

Experimental structures · PDB · 1

• 3LWB X-ray 2.10A

A predicted model is available from AlphaFold.

Gene Ontology · 8

• GO:0005829 cytosol
• GO:0005886 plasma membrane
• GO:0005524 ATP binding
• GO:0008716 D-alanine-D-alanine ligase activity
• GO:0046872 metal ion binding
• GO:0071555 cell wall organization
• GO:0009252 peptidoglycan biosynthetic process
• GO:0008360 regulation of cell shape

Drugs targeting this protein · 1

• CYCLOSERINE inhibitor

Related proteins · sequence + function similarity

• D-alanine--D-alanine ligase 1.00
• D-alanine--D-alanine ligase 1.00
• D-alanine--D-alanine ligase 1.00
• D-alanine--D-alanine ligase 0.97
• D-alanine--D-alanine ligase 0.95
• D-alanine--D-alanine ligase 0.95
• D-alanine--D-alanine ligase 0.95
• D-alanine--D-alanine ligase 0.95
• D-alanine--D-alanine ligase 0.94
• D-alanine--D-alanine ligase 0.94
• D-alanine--D-alanine ligase 0.94
• D-alanine--D-alanine ligase 0.93

Literature · 4 cited papers

• Kinetic mechanism and inhibition of Mycobacterium tuberculosis D-alanine:D-alanine ligase by the antibiotic D-cycloserine. FEBS J. · 2013
• Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Mol. Cell. Proteomics · 2011
• Structure of the Mycobacterium tuberculosis D-alanine:D-alanine ligase, a target of the antituberculosis drug D-cycloserine. Antimicrob. Agents Chemother. · 2011
• Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature · 1998