Bifunctional epoxide hydrolase 2

Function

Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides (By similarity). Also determines steady-state levels of physiological mediators.

Classification

Family (Pfam)

PF00561 Abhydrolase_1, PF00702 Hydrolase

InterPro

AB_hydrolase_1, AB_hydrolase_fold, Epox_hydrolase-like, HAD-like_sf, HAD-SF_hydro_IA, HAD-SF_ppase_IA/epoxid_hydro_N, HAD_sf, PGP-like_dom2

Functional cluster

Oxidoreductases & Polyketide Synthases

Experimental structures · PDB · 129

• 1S8O X-ray 2.60A
• 1VJ5 X-ray 2.35A
• 1ZD2 X-ray 3.00A
• 1ZD3 X-ray 2.30A
• 1ZD4 X-ray 2.70A
• 1ZD5 X-ray 2.60A
• 3ANS X-ray 1.98A
• 3ANT X-ray 2.40A
• 3I1Y X-ray 2.47A
• 3I28 X-ray 1.95A
• 3KOO X-ray 2.79A
• 3OTQ X-ray 3.00A
• … and 117 more

A predicted model is available from AlphaFold.

Gene Ontology · 21

• GO:0005829 cytosol
• GO:0070062 extracellular exosome
• GO:0005782 peroxisomal matrix
• GO:0005777 peroxisome
• GO:0033885 10-hydroxy-9-(phosphonooxy)octadecanoate phosphatase activity
• GO:0004301 epoxide hydrolase activity
• GO:0042577 lipid phosphatase activity
• GO:0052642 lysophosphatidic acid phosphatase activity
• GO:0000287 magnesium ion binding
• GO:0016791 phosphatase activity
• GO:0042803 protein homodimerization activity
• GO:0015643 toxic substance binding
• GO:0042632 cholesterol homeostasis
• GO:0016311 dephosphorylation
• GO:0097176 epoxide metabolic process
• GO:0046839 phospholipid dephosphorylation
• GO:0010628 positive regulation of gene expression
• GO:0001558 regulation of cell growth
• GO:0090181 regulation of cholesterol metabolic process
• GO:0009636 response to toxic substance
• GO:0046272 stilbene catabolic process

Drugs targeting this protein · 2

• GSK2256294 inhibitor
• AR9281 inhibitor

Related proteins · sequence + function similarity

• Bifunctional epoxide hydrolase 2 0.97
• Bifunctional epoxide hydrolase 2 0.97
• Bifunctional epoxide hydrolase 2 0.96
• Epoxide hydrolase 2 0.69
• Epoxide hydrolase 3 0.67
• Epoxide hydrolase 1 0.65
• Pseudouridine-5'-phosphatase 0.65
• Epoxide hydrolase 3 0.63
• Pseudouridine-5'-phosphatase 0.63
• Acyl-CoA dehydrogenase family member 10 0.60
• Haloacid dehalogenase-like hydrolase domain-containing protein 3 0.60
• N-acylneuraminate-9-phosphatase 0.58

Co-cited proteins · studied together in the literature

• Bifunctional epoxide hydrolase 2 2 shared papers
• Bifunctional epoxide hydrolase 2 1 shared papers
• Epoxide hydrolase 3 1 shared papers
• Epoxide hydrolase 3 1 shared papers
• Epoxide hydrolase 1 1 shared papers

Literature · 23 cited papers

• An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
• EH3 (ABHD9): the first member of a new epoxide hydrolase family with high activity for fatty acid epoxides. J. Lipid Res. · 2012
• Role of soluble epoxide hydrolase phosphatase activity in the metabolism of lysophosphatidic acids. Biochem. Biophys. Res. Commun. · 2012
• Lysophosphatidic acids are new substrates for the phosphatase domain of soluble epoxide hydrolase. J. Lipid Res. · 2012
• Initial characterization of the human central proteome. BMC Syst. Biol. · 2011
• Mammalian soluble epoxide hydrolase is identical to liver hepoxilin hydrolase. J. Lipid Res. · 2011
• Redox regulation of soluble epoxide hydrolase by 15-deoxy-delta-prostaglandin J2 controls coronary hypoxic vasodilation. Circ. Res. · 2011
• Substituted pyrazoles as novel sEH antagonist: investigation of key binding interactions within the catalytic domain. Bioorg. Med. Chem. Lett. · 2010
• Optimization of piperidyl-ureas as inhibitors of soluble epoxide hydrolase. Bioorg. Med. Chem. Lett. · 2010
• Structure-based optimization of arylamides as inhibitors of soluble epoxide hydrolase. J. Med. Chem. · 2009
• Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science · 2009
• DNA sequence and analysis of human chromosome 8. Nature · 2006
• … and 11 more in the literature graph