Mitogen-activated protein kinase HOG1

Function

Proline-directed serine/threonine-protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription via the stress response element (STRE) in promoters of target genes. Upon osmotic shock, associates with the SKO1-SSN6-TUP1 complex, phosphorylates SKO1, and converts it into an activator that subsequently recruits Swi/Snf and SAGA complexes. Activates the SMP1 transcription factor and the RCK2 kinase, both also involved in the regulation of the expression of a subset of osmotic stress-related genes. Phosphorylation of HSL1 by HOG1 leads to a G2 arrest essential for cell survival at high osmolarity. Also mediates cell-cycle arrest in G1 phase by the dual targeting of SIC1. Phosphorylates methyltransferase DOT1 at least on 'Ser-565' and 'Thr-576'. Regulates MFA2 ARE-mediated translation in response to carbon source. Targets RPD3 histone deacetylase to osmoresponsive promoters to induce gene expression on stress. Required for the Golgi apparatus localization of MNN1. Plays an essential role in maintaining water homeostasis, arsenite detoxification, copper-resistance, cold-resistance, hydrogen peroxide response, adaptation to citric acid stress, and repression of the mating pathway activity. Functions as an arsenic sensor and effector via direct binding to arsenic and subsequent phosphorylation of the ARR1 transcription factor.

Classification

Family (Pfam)

PF00069 Pkinase

InterPro

Kinase-like_dom_sf, MAP_kinase_CS, MAPK, MAPK_HOG-like, MAPK_Sty1/HOG1, Prot_kinase_dom, Protein_kinase_ATP_BS, Ser/Thr_kinase_AS

Functional cluster

Protein Serine/Threonine Kinases

Gene Ontology · 20

• GO:0005737 cytoplasm
• GO:0005634 nucleus
• GO:0005524 ATP binding
• GO:0005516 calmodulin binding
• GO:0003682 chromatin binding
• GO:0004707 MAP kinase activity
• GO:0106310 protein serine kinase activity
• GO:0004674 protein serine/threonine kinase activity
• GO:0071474 cellular hyperosmotic response
• GO:0071470 cellular response to osmotic stress
• GO:0034599 cellular response to oxidative stress
• GO:0006351 DNA-templated transcription
• GO:0006972 hyperosmotic response
• GO:0001100 negative regulation of exit from mitosis
• GO:0007231 osmosensory signaling pathway
• GO:0045944 positive regulation of transcription by RNA polymerase II
• GO:0016241 regulation of macroautophagy
• GO:0033262 regulation of nuclear cell cycle DNA replication
• GO:0006357 regulation of transcription by RNA polymerase II
• GO:0051403 stress-activated MAPK cascade

Neighborhood · nearest proteins

Related proteins · sequence + function similarity

• Mitogen-activated protein kinase HOG1 0.98
• Mitogen-activated protein kinase HOG1 0.98
• Mitogen-activated protein kinase HOG1 0.97
• Mitogen-activated protein kinase HOG1 0.97
• Mitogen-activated protein kinase HOG1 0.96
• Mitogen-activated protein kinase HOG2 0.94
• Mitogen-activated protein kinase HOG1 0.93
• Mitogen-activated protein kinase HOG1 0.93
• Mitogen-activated protein kinase HOG1 0.92
• Mitogen-activated protein kinase HOG1 0.91
• Mitogen-activated protein kinase hog1 0.91
• Mitogen-activated protein kinase HOG1 0.90

Co-cited proteins · studied together in the literature

• High osmolarity signaling protein SHO1 5 shared papers
• High-osmolarity-induced transcription protein 1 2 shared papers
• Tyrosine-protein phosphatase 2 2 shared papers
• Tyrosine-protein phosphatase 3 2 shared papers
• Protein SIC1 1 shared papers
• Serine/threonine-protein kinase RCK2 1 shared papers
• MAP kinase kinase PBS2 4 shared papers
• Transcription factor SMP1 1 shared papers
• Arsenical-resistance protein ARR1 1 shared papers
• Hsp90 co-chaperone Cdc37 1 shared papers
• Glycerol-3-phosphate dehydrogenase [NAD(+)] 1 1 shared papers
• Guanine nucleotide-binding protein alpha-1 subunit 1 shared papers

Literature · 57 cited papers

• Proline-directed yeast and human MAP kinases phosphorylate the Dot1p/DOT1L histone H3K79 methyltransferase. FEBS J. · 2024
• Thiol-based direct threat sensing by the stress-activated protein kinase Hog1. Sci. Signal. · 2019
• The reference genome sequence of Saccharomyces cerevisiae: Then and now. G3 (Bethesda) · 2014
• N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc. Natl. Acad. Sci. U.S.A. · 2012
• Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution. Science · 2009
• A multidimensional chromatography technology for in-depth phosphoproteome analysis. Mol. Cell. Proteomics · 2008
• Plc1p is required for SAGA recruitment and derepression of Sko1p-regulated genes. Mol. Biol. Cell · 2007
• A systems-biology analysis of feedback inhibition in the Sho1 osmotic-stress-response pathway. Curr. Biol. · 2007
• Dissecting yeast Hog1 MAP kinase pathway using a chemical genetic approach. FEBS Lett. · 2007
• Cdc37p is required for stress-induced high-osmolarity glycerol and protein kinase C mitogen-activated protein kinase pathway functionality by interaction with Hog1p and Slt2p (Mpk1p). Eukaryot. Cell · 2007
• Mitogen-activated protein kinase Hog1 is essential for the response to arsenite in Saccharomyces cerevisiae. Eukaryot. Cell · 2006
• Analysis of mitogen-activated protein kinase signaling specificity in response to hyperosmotic stress: use of an analog-sensitive HOG1 allele. Eukaryot. Cell · 2006
• … and 45 more in the literature graph