Nitric oxide synthase 1

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

Classification

Family (Pfam)

PF00667 FAD_binding_1, PF00258 Flavodoxin_1, PF00175 NAD_binding_1, PF02898 NO_synthase, PF00595 PDZ

InterPro

CysJ-like_FAD-binding, FAD-bd_FR_type, Flavdoxin-like, Flavodoxin/NO_synth, Flavoprot_Pyr_Nucl_cyt_Rdtase, Flavoprotein-like_sf, FNR_nucleotide-bd, NADPH_Cyt_P450_Rdtase_alpha, NOS, NOS_dom_1, NOS_dom_2, NOS_dom_3, NOS_euk, NOS_N, NOS_N_sf, OxRdtase_FAD/NAD-bd, PDZ, PDZ_sf, Riboflavin_synthase-like_b-brl

Functional cluster

Viral Structural & Replication Proteins

Experimental structures · PDB · 115

• 4D1N X-ray 2.03A
• 4UCH X-ray 2.20A
• 4UH5 X-ray 1.98A
• 4UH6 X-ray 1.98A
• 4V3U X-ray 2.30A
• 5ADF X-ray 1.97A
• 5ADG X-ray 1.98A
• 5ADI X-ray 2.20A
• 5FVU X-ray 2.22A
• 5FVV X-ray 2.05A
• 5FVW X-ray 2.20A
• 5FVX X-ray 2.30A
• … and 103 more

A predicted model is available from AlphaFold.

Gene Ontology · 69

• GO:0044305 calyx of Held
• GO:0005901 caveola
• GO:0071944 cell periphery
• GO:0005737 cytoplasm
• GO:0005856 cytoskeleton
• GO:0005829 cytosol
• GO:0043197 dendritic spine
• GO:0045121 membrane raft
• GO:0005739 mitochondrion
• GO:0005634 nucleus
• GO:0048471 perinuclear region of cytoplasm
• GO:0001917 photoreceptor inner segment
• GO:0005886 plasma membrane
• GO:0014069 postsynaptic density
• GO:0032991 protein-containing complex
• GO:0042383 sarcolemma
• GO:0016529 sarcoplasmic reticulum
• GO:0033017 sarcoplasmic reticulum membrane
• GO:0045202 synapse
• GO:0030315 T-tubule
• GO:0030018 Z disc
• GO:0034618 arginine binding
• GO:0046870 cadmium ion binding
• GO:0005246 calcium channel regulator activity
• GO:0048306 calcium-dependent protein binding
• GO:0005516 calmodulin binding
• GO:0050660 flavin adenine dinucleotide binding
• GO:0010181 FMN binding
• GO:0020037 heme binding
• GO:0050661 NADP binding
• GO:0004517 nitric-oxide synthase activity
• GO:0035605 peptidyl-cysteine S-nitrosylase activity
• GO:0097110 scaffold protein binding
• GO:0017080 sodium channel regulator activity
• GO:0034617 tetrahydrobiopterin binding
• GO:0044325 transmembrane transporter binding
• GO:0045454 cell redox homeostasis
• GO:0071363 cellular response to growth factor stimulus
• GO:0006527 L-arginine catabolic process
• GO:0033555 multicellular organismal response to stress
• GO:0007520 myoblast fusion
• GO:0045776 negative regulation of blood pressure
• GO:0051926 negative regulation of calcium ion transport
• GO:0010523 negative regulation of calcium ion transport into cytosol
• GO:0043267 negative regulation of potassium ion transport
• GO:0051612 negative regulation of serotonin uptake
• GO:0006809 nitric oxide biosynthetic process
• GO:0007263 nitric oxide mediated signal transduction
• GO:0106071 positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway
• GO:0045893 positive regulation of DNA-templated transcription
• GO:1905026 positive regulation of membrane repolarization during ventricular cardiac muscle cell action potential
• GO:0043525 positive regulation of neuron apoptotic process
• GO:1902307 positive regulation of sodium ion transmembrane transport
• GO:0098735 positive regulation of the force of heart contraction
• GO:0045944 positive regulation of transcription by RNA polymerase II
• GO:1902514 regulation of calcium ion transmembrane transport via high voltage-gated calcium channel
• GO:0055117 regulation of cardiac muscle contraction
• GO:0010882 regulation of cardiac muscle contraction by calcium ion signaling
• GO:0050767 regulation of neurogenesis
• GO:0060078 regulation of postsynaptic membrane potential
• GO:0002028 regulation of sodium ion transport
• GO:0009408 response to heat
• GO:0009725 response to hormone
• GO:0001666 response to hypoxia
• GO:0032496 response to lipopolysaccharide
• GO:0006941 striated muscle contraction
• GO:0099163 synaptic signaling by nitric oxide
• GO:0042311 vasodilation
• GO:0042178 xenobiotic catabolic process

Drugs targeting this protein · 2

• TILARGININE ACETATE inhibitor
• TILARGININE inhibitor

Related proteins · sequence + function similarity

• Nitric oxide synthase 1 0.98
• Nitric oxide synthase 1 0.97
• Nitric oxide synthase 1 0.96
• Nitric oxide synthase 1 0.94
• Nitric oxide synthase 0.72
• Nitric oxide synthase 0.69
• Protein Nef 0.64
• Protein Nef 0.64
• Protein Nef 0.64
• Nitric oxide synthase 0.64
• ORFB polyprotein 0.64
• Protein Nef 0.63

Co-cited proteins · studied together in the literature

• Nitric oxide synthase 3 2 shared papers
• Nitric oxide synthase, inducible 1 shared papers
• Nitric oxide synthase 1 1 shared papers

Literature · 9 cited papers

• 2-Aminopyridines with a shortened amino sidechain as potent, selective, and highly permeable human neuronal nitric oxide synthase inhibitors. Bioorg. Med. Chem. · 2022
• Structures of human constitutive nitric oxide synthases. Acta Crystallogr. D · 2014
• The finished DNA sequence of human chromosome 12. Nature · 2006
• Isolation and characterization of a novel, human neuronal nitric oxide synthase cDNA. Biochem. Biophys. Res. Commun. · 1998
• A novel, testis-specific mRNA transcript encoding an NH2-terminal truncated nitric-oxide synthase. J. Biol. Chem. · 1997
• Neuronal isoform of nitric oxide synthase is expressed at low levels in human retina. Cell. Mol. Neurobiol. · 1996
• Cloned human brain nitric oxide synthase is highly expressed in skeletal muscle. FEBS Lett. · 1993
• Structural organization of the human neuronal nitric oxide synthase gene (NOS1). J. Biol. Chem. · 1994
• Expression of two types of nitric oxide synthase mRNA in human neuroblastoma cell lines. J. Neurochem. · 1994