Cytosol aminopeptidase

Function

Cytosolic metallopeptidase that catalyzes the removal of unsubstituted N-terminal hydrophobic amino acids from various peptides. The presence of Zn(2+) ions is essential for the peptidase activity, and the association with other cofactors can modulate the substrate spectificity of the enzyme. For instance, in the presence of Mn(2+), it displays a specific Cys-Gly hydrolyzing activity of Cys-Gly-S-conjugates. Involved in the metabolism of glutathione and in the degradation of glutathione S-conjugates, which may play a role in the control of the cell redox status.

Classification

Family (Pfam)

PF00883 Peptidase_M17, PF02789 Peptidase_M17_N

InterPro

Leucine_aapep/pepB, Macro_dom-like, Peptidase_M17_C, Peptidase_M17_leu_NH2_pept, Peptidase_M17_N

Functional cluster

Tubulins & Mitochondrial Metabolic Enzymes

Gene Ontology · 13

• GO:0005737 cytoplasm
• GO:0070062 extracellular exosome
• GO:0005925 focal adhesion
• GO:0005739 mitochondrion
• GO:0005634 nucleus
• GO:0005802 trans-Golgi network
• GO:0004177 aminopeptidase activity
• GO:0004180 carboxypeptidase activity
• GO:0030145 manganese ion binding
• GO:0070006 metalloaminopeptidase activity
• GO:0008235 metalloexopeptidase activity
• GO:0008233 peptidase activity
• GO:0006508 proteolysis

Drugs targeting this protein · 1

• TOSEDOSTAT inhibitor

Related proteins · sequence + function similarity

• Cytosol aminopeptidase 0.99
• Cytosol aminopeptidase 0.99
• Cytosol aminopeptidase 0.98
• Cytosol aminopeptidase 0.98
• Cytosol aminopeptidase 0.97
• Putative aminopeptidase C13A11.05 0.81
• Crystallomitin S-Lap2 0.75
• Cytosol aminopeptidase 0.73
• Crystallomitin S-Lap1 0.72
• Putative leucine aminopeptidase 1 0.72
• Crystallomitin S-Lap5 0.71
• Leucine aminopeptidase 1 0.71

Co-cited proteins · studied together in the literature

• Cytosol aminopeptidase 1 shared papers

Literature · 9 cited papers

• N-terminome analysis of the human mitochondrial proteome. Proteomics · 2015
• An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
• Toward a comprehensive characterization of a human cancer cell phosphoproteome. J. Proteome Res. · 2013
• Initial characterization of the human central proteome. BMC Syst. Biol. · 2011
• Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci. Signal. · 2010
• Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science · 2009
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. · 2004
• Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat. Genet. · 2004
• Structural and immunological evidence for the identity of prolyl aminopeptidase with leucyl aminopeptidase. Biochem. Biophys. Res. Commun. · 1991