Chymase

Function

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.

Classification

Family (Pfam)

PF00089 Trypsin

InterPro

Peptidase_S1_PA, Peptidase_S1_PA_chymotrypsin, Peptidase_S1A, Trypsin_dom, TRYPSIN_HIS, TRYPSIN_SER

Functional cluster

Venom Serine Proteases & Phospholipase A2

Experimental structures · PDB · 42

• 1KLT X-ray 1.90A
• 1NN6 X-ray 1.75A
• 1PJP X-ray 2.20A
• 1T31 X-ray 1.90A
• 2HVX X-ray 2.60A
• 3N7O X-ray 1.80A
• 3S0N X-ray 1.95A
• 4AFQ X-ray 1.51A
• 4AFS X-ray 1.90A
• 4AFU X-ray 1.82A
• 4AFZ X-ray 2.25A
• 4AG1 X-ray 1.40A
• … and 30 more

A predicted model is available from AlphaFold.

Gene Ontology · 20

• GO:0036464 cytoplasmic ribonucleoprotein granule
• GO:0031012 extracellular matrix
• GO:0005576 extracellular region
• GO:0005615 extracellular space
• GO:0030141 secretory granule
• GO:0004175 endopeptidase activity
• GO:0042277 peptide binding
• GO:0004252 serine-type endopeptidase activity
• GO:0008236 serine-type peptidase activity
• GO:0002003 angiotensin maturation
• GO:0034769 basement membrane disassembly
• GO:0071333 cellular response to glucose stimulus
• GO:0140447 cytokine precursor processing
• GO:0022617 extracellular matrix disassembly
• GO:0030901 midbrain development
• GO:0006518 peptide metabolic process
• GO:0045766 positive regulation of angiogenesis
• GO:0030163 protein catabolic process
• GO:0051604 protein maturation
• GO:0050727 regulation of inflammatory response

Drugs targeting this protein · 1

• FULACIMSTAT inhibitor

Related proteins · sequence + function similarity

• Chymase 1.00
• Chymase 1.00
• Chymase 0.98
• Chymase 0.98
• Mast cell protease 2 0.97
• Chymase 0.96
• Mast cell protease 2 0.96
• Granzyme H 0.95
• Mast cell protease 1 0.95
• Chymase 0.95
• Mast cell protease 4 0.94
• Mast cell protease 3 0.94

Literature · 12 cited papers

• Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J. Proteome Res. · 2009
• Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nat. Methods · 2008
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. · 2004
• The DNA sequence and analysis of human chromosome 14. Nature · 2003
• PMID 10208809 J. Mol. Biol. · 1999
• The 2.2-A crystal structure of human chymase in complex with succinyl-Ala-Ala-Pro-Phe-chloromethylketone: structural explanation for its dipeptidyl carboxypeptidase specificity. J. Mol. Biol. · 1999
• Crystal structure of phenylmethanesulfonyl fluoride-treated human chymase at 1.9 A. Biochemistry · 1997
• Purification and molecular cloning of chymase from human tonsils. FEBS Lett. · 1993
• Determination of the primary structures of human skin chymase and cathepsin G from cutaneous mast cells of urticaria pigmentosa lesions. J. Immunol. · 1994
• Structure, chromosomal assignment, and deduced amino acid sequence of a human gene for mast cell chymase. J. Biol. Chem. · 1991
• Angiotensin II-forming heart chymase is a mast-cell-specific enzyme. Biochem. J. · 1991
• Cloning of the gene and cDNA for human heart chymase. J. Biol. Chem. · 1991