POU domain, class 5, transcription factor 1
Also known as: Oct-3, Oct-4, Otf-3, Otf3, Pou5f1
Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Classification
- Family (Pfam)
- PF00046 Homeodomain, PF00157 Pou
- InterPro
- HD, Homeobox_CS, Homeodomain-like_sf, Lambda_DNA-bd_dom_sf, POU, POU_dom, POU_domain_TF
- Functional cluster
- Homeobox & Zinc-Finger Transcription Factors
Experimental structures · PDB · 5
A predicted model is available from AlphaFold.
Gene Ontology · 62
- GO:0000785 chromatin
- GO:0005737 cytoplasm
- GO:0005829 cytosol
- GO:0005739 mitochondrion
- GO:0005730 nucleolus
- GO:0005654 nucleoplasm
- GO:0005634 nucleus
- GO:0005667 transcription regulator complex
- GO:0017053 transcription repressor complex
- GO:0003682 chromatin binding
- GO:0031490 chromatin DNA binding
- GO:0000987 cis-regulatory region sequence-specific DNA binding
- GO:0019955 cytokine binding
- GO:0003677 DNA binding
- GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific
- GO:0003700 DNA-binding transcription factor activity
- GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
- GO:0140297 DNA-binding transcription factor binding
- GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific
- GO:0140129 histone H3K56ac reader activity
- GO:0071837 HMG box domain binding
- GO:0035198 miRNA binding
- GO:0004879 nuclear receptor activity
- GO:0070974 POU domain binding
- GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
- GO:0001162 RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding
- GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding
- GO:0043565 sequence-specific DNA binding
- GO:1990837 sequence-specific double-stranded DNA binding
- GO:0000976 transcription cis-regulatory region binding
- GO:0031625 ubiquitin protein ligase binding
- GO:0001824 blastocyst development
- GO:0001832 blastocyst growth
- GO:0030154 cell differentiation
- GO:0045165 cell fate commitment
- GO:1990830 cellular response to leukemia inhibitory factor
- GO:0001712 ectodermal cell fate commitment
- GO:0001711 endodermal cell fate commitment
- GO:0001714 endodermal cell fate specification
- GO:0010467 gene expression
- GO:0030718 germ-line stem cell population maintenance
- GO:0001710 mesodermal cell fate commitment
- GO:0045955 negative regulation of calcium ion-dependent exocytosis
- GO:0045596 negative regulation of cell differentiation
- GO:0045892 negative regulation of DNA-templated transcription
- GO:0010629 negative regulation of gene expression
- GO:0051898 negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
- GO:0000122 negative regulation of transcription by RNA polymerase II
- GO:0045893 positive regulation of DNA-templated transcription
- GO:0010628 positive regulation of gene expression
- GO:0045944 positive regulation of transcription by RNA polymerase II
- GO:0060261 positive regulation of transcription initiation by RNA polymerase II
- GO:0009786 regulation of asymmetric cell division
- GO:0006355 regulation of DNA-templated transcription
- GO:0006357 regulation of transcription by RNA polymerase II
- GO:0080021 response to benzoic acid
- GO:0032526 response to retinoic acid
- GO:0048863 stem cell differentiation
- GO:0019827 stem cell population maintenance
- GO:0000723 telomere maintenance
- GO:0006366 transcription by RNA polymerase II
- GO:0001829 trophectodermal cell differentiation
Neighborhood · nearest proteins
Related proteins · sequence + function similarity
- POU domain, class 5, transcription factor 1 0.99
- POU domain, class 5, transcription factor 1 0.98
- POU domain, class 5, transcription factor 1 0.98
- POU domain, class 5, transcription factor 1 0.98
- POU domain, class 5, transcription factor 1B 0.97
- POU domain, class 5, transcription factor 1 0.97
- POU domain, class 5, transcription factor 1.3 0.88
- POU domain, class 5, transcription factor 1.3 0.85
- Dorsal root ganglia homeobox protein 0.84
- Pancreas/duodenum homeobox protein 1 0.84
- Dorsal root ganglia homeobox protein 0.83
- Dorsal root ganglia homeobox protein 0.83
Co-cited proteins · studied together in the literature
- Zinc finger protein 322 1 shared papers
- Transcription factor SOX-2 5 shared papers
- Nuclear receptor subfamily 6 group A member 1 2 shared papers
- Probable ATP-dependent RNA helicase DDX56 1 shared papers
- Zinc finger protein 143 1 shared papers
- NEDD4-like E3 ubiquitin-protein ligase WWP2 1 shared papers
- Nuclear receptor subfamily 5 group A member 2 2 shared papers
- Steroid hormone receptor ERR2 1 shared papers
- Zinc finger and SCAN domain-containing protein 10 1 shared papers
- Transcription factor SOX-15 1 shared papers
- F-box/WD repeat-containing protein 4 1 shared papers
- Krueppel-like factor 4 1 shared papers
Literature · 22 cited papers
- Ddx56 maintains proliferation of mouse embryonic stem cells via ribosome assembly and interaction with the Oct4/Sox2 complex. Stem Cell Res Ther · 2020
- Serine 347 Phosphorylation by JNKs Negatively Regulates OCT4 Protein Stability in Mouse Embryonic Stem Cells. Stem Cell Reports · 2017
- A Structural investigation into Oct4 regulation by orphan nuclear receptors, germ cell nuclear factor (GCNF), and liver receptor homolog-1 (LRH-1). J. Mol. Biol. · 2016
- Zfp322a Regulates mouse ES cell pluripotency and enhances reprogramming efficiency. PLoS Genet. · 2014
- A unique Oct4 interface is crucial for reprogramming to pluripotency. Nat. Cell Biol. · 2013
- Wwp2 mediates Oct4 ubiquitination and its own auto-ubiquitination in a dosage-dependent manner. Cell Res. · 2010
- Zfp206, Oct4, and Sox2 are integrated components of a transcriptional regulatory network in embryonic stem cells. J. Biol. Chem. · 2009
- Zfp143 regulates Nanog through modulation of Oct4 binding. Stem Cells · 2008
- Estrogen-related receptor beta interacts with Oct4 to positively regulate Nanog gene expression. Mol. Cell. Biol. · 2008
- Sumoylation of Oct4 enhances its stability, DNA binding, and transactivation. J. Biol. Chem. · 2007
- Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1. FASEB J. · 2007
- Inhibition of DNA binding of Sox2 by the SUMO conjugation. Biochem. Biophys. Res. Commun. · 2006
- … and 10 more in the literature graph
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