Nuclear factor NF-kappa-B p105 subunit
Also known as: NFKB1
Function
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
Classification
- Family (Pfam)
- PF12796 Ank_2, PF00531 Death, PF16179 RHD_dimer, PF00554 RHD_DNA_bind
- InterPro
- Ankyrin_rpt, Ankyrin_rpt-contain_sf, DEATH-like_dom_sf, Death_dom, Ig-like_fold, Ig_E-set, IPT_dom, IPT_NFkappaB, NF-kB_p105_DD, NF-kB_p105_RHD_N, NFkB/Dor, p53-like_TF_DNA-bd_sf, RHD_CS, RHD_dimer, RHD_DNA_bind_dom, RHD_DNA_bind_dom_sf
- Functional cluster
- Homeobox & Zinc-Finger Transcription Factors
Experimental structures · PDB · 15
- 1MDI NMR
- 1MDJ NMR
- 1MDK NMR
- 1NFI X-ray 2.70A
- 1SVC X-ray 2.60A
- 2DBF NMR
- 2O61 X-ray 2.80A
- 3GUT X-ray 3.59A
- 7LEQ X-ray 2.24A
- 7LET X-ray 2.40A
- 7LF4 X-ray 2.85A
- 7LFC X-ray 2.10A
- … and 3 more
A predicted model is available from AlphaFold.
Gene Ontology · 54
- GO:0000785 chromatin
- GO:0005737 cytoplasm
- GO:0005829 cytosol
- GO:0005576 extracellular region
- GO:0033256 I-kappaB/NF-kappaB complex
- GO:0035525 NF-kappaB p50/p65 complex
- GO:0005654 nucleoplasm
- GO:0005634 nucleus
- GO:0034774 secretory granule lumen
- GO:0035580 specific granule lumen
- GO:0042805 actinin binding
- GO:0003682 chromatin binding
- GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific
- GO:0003700 DNA-binding transcription factor activity
- GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
- GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific
- GO:0042802 identical protein binding
- GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
- GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding
- GO:0000976 transcription cis-regulatory region binding
- GO:0003712 transcription coregulator activity
- GO:0140367 antibacterial innate immune response
- GO:0006915 apoptotic process
- GO:0050853 B cell receptor signaling pathway
- GO:0007249 canonical NF-kappaB signal transduction
- GO:1904385 cellular response to angiotensin
- GO:0097398 cellular response to interleukin-17
- GO:0071354 cellular response to interleukin-6
- GO:0071222 cellular response to lipopolysaccharide
- GO:0071260 cellular response to mechanical stimulus
- GO:0071316 cellular response to nicotine
- GO:0098586 cellular response to virus
- GO:0006954 inflammatory response
- GO:0007254 JNK cascade
- GO:0060056 mammary gland involution
- GO:0043066 negative regulation of apoptotic process
- GO:1900016 negative regulation of cytokine production involved in inflammatory response
- GO:0010629 negative regulation of gene expression
- GO:0050728 negative regulation of inflammatory response
- GO:0000122 negative regulation of transcription by RNA polymerase II
- GO:0010957 negative regulation of vitamin D biosynthetic process
- GO:0038061 non-canonical NF-kappaB signal transduction
- GO:0090263 positive regulation of canonical Wnt signaling pathway
- GO:0010875 positive regulation of cholesterol efflux
- GO:0045893 positive regulation of DNA-templated transcription
- GO:1900127 positive regulation of hyaluronan biosynthetic process
- GO:0010884 positive regulation of lipid storage
- GO:0010744 positive regulation of macrophage derived foam cell differentiation
- GO:2000630 positive regulation of miRNA metabolic process
- GO:0045944 positive regulation of transcription by RNA polymerase II
- GO:0060261 positive regulation of transcription initiation by RNA polymerase II
- GO:0006357 regulation of transcription by RNA polymerase II
- GO:0006366 transcription by RNA polymerase II
- GO:0033209 tumor necrosis factor-mediated signaling pathway
Disease associations
- immunodeficiency, common variable, 12 MONDO:0014697
Drugs targeting this protein · 1
- EDASALONEXENT inhibitor
Neighborhood · nearest proteins
Related proteins · sequence + function similarity
- Nuclear factor NF-kappa-B p105 subunit 0.99
- Nuclear factor NF-kappa-B p105 subunit 0.99
- Nuclear factor NF-kappa-B p105 subunit 0.98
- Nuclear factor NF-kappa-B p105 subunit 0.93
- Nuclear factor NF-kappa-B p100 subunit 0.86
- Nuclear factor NF-kappa-B p100 subunit 0.85
- Nuclear factor NF-kappa-B p100 subunit 0.79
- Nuclear factor NF-kappa-B p100 subunit 0.79
- Nuclear factor NF-kappa-B p110 subunit 0.70
- Transcription factor RelB homolog 0.63
- Phosphoinositide 3-kinase adapter protein 1 0.63
- Phosphoinositide 3-kinase adapter protein 1 0.62
Co-cited proteins · studied together in the literature
- Proto-oncogene c-Rel 4 shared papers
- Mitogen-activated protein kinase kinase kinase 8 1 shared papers
- B-cell lymphoma 3 protein 1 shared papers
- Transcription factor p65 8 shared papers
- TSC22 domain family protein 3 2 shared papers
- Mitogen-activated protein kinase kinase kinase 8 2 shared papers
- TSC22 domain family protein 3 2 shared papers
- E3 ubiquitin-protein ligase RNF123 1 shared papers
- UNC5C-like protein 1 shared papers
- UNC5C-like protein 1 shared papers
- Nuclear factor NF-kappa-B p105 subunit 1 shared papers
- F-box/WD repeat-containing protein 1A 3 shared papers
Literature · 51 cited papers
- FOXM1D potentiates PKM2-mediated tumor glycolysis and angiogenesis. Mol. Oncol. · 2021
- Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation. Nat. Struct. Mol. Biol. · 2017
- Haploinsufficiency of the NF-kappaB1 Subunit p50 in Common Variable Immunodeficiency. Am. J. Hum. Genet. · 2015
- KPC1-mediated ubiquitination and proteasomal processing of NF-kappaB1 p105 to p50 restricts tumor growth. Cell · 2015
- An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
- Toward a comprehensive characterization of a human cancer cell phosphoproteome. J. Proteome Res. · 2013
- Initial characterization of the human central proteome. BMC Syst. Biol. · 2011
- Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci. Signal. · 2009
- A quantitative atlas of mitotic phosphorylation. Proc. Natl. Acad. Sci. U.S.A. · 2008
- The full-ORF clone resource of the German cDNA consortium. BMC Genomics · 2007
- Posttranslational hydroxylation of ankyrin repeats in IkappaB proteins by the hypoxia-inducible factor (HIF) asparaginyl hydroxylase, factor inhibiting HIF (FIH). Proc. Natl. Acad. Sci. U.S.A. · 2006
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. · 2004
- … and 39 more in the literature graph