Nucleolin

Function

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Classification

Family (Pfam)

PF00076 RRM_1

InterPro

Nucleolin_RRM1, Nucleolin_RRM2, Nucleolin_RRM3, Nucleolin_RRM4, Nucleotide-bd_a/b_plait_sf, RBD_domain_sf, RRM_dom, RRM_euk-type

Functional cluster

Adenylate Kinases & Peroxiredoxins

Experimental structures · PDB · 4

• 2FC8 NMR
• 2FC9 NMR
• 2KRR NMR
• 9CB5 X-ray 2.60A

A predicted model is available from AlphaFold.

Gene Ontology · 24

• GO:0005938 cell cortex
• GO:0005694 chromosome
• GO:0001533 cornified envelope
• GO:0036464 cytoplasmic ribonucleoprotein granule
• GO:0070062 extracellular exosome
• GO:0160056 macropinosome membrane
• GO:0016020 membrane
• GO:0005730 nucleolus
• GO:0005654 nucleoplasm
• GO:0005634 nucleus
• GO:0005886 plasma membrane
• GO:1990904 ribonucleoprotein complex
• GO:0005681 spliceosomal complex
• GO:0044547 DNA topoisomerase binding
• GO:0042802 identical protein binding
• GO:0043560 insulin receptor substrate binding
• GO:0048027 mRNA 5'-UTR binding
• GO:0042731 PH domain binding
• GO:0003723 RNA binding
• GO:0042162 telomeric DNA binding
• GO:0001525 angiogenesis
• GO:0000398 mRNA splicing, via spliceosome
• GO:0017148 negative regulation of translation
• GO:1901838 positive regulation of transcription of nucleolar large rRNA by RNA polymerase I

Neighborhood · nearest proteins

Related proteins · sequence + function similarity

• Nucleolin 1.00
• Nucleolin 1.00
• Nucleolin 0.99
• Nucleolin 0.99
• Nucleolin 0.99
• Nucleolin 0.97
• Nucleolin 0.96
• Nucleolin 1 0.89
• Nucleolin 1 0.89
• Nucleolin 2 0.85
• Nucleolin homolog 1 0.84
• Nuclear localization sequence-binding protein 0.81

Co-cited proteins · studied together in the literature

• GDNF-inducible zinc finger protein 1 1 shared papers
• Insulin-like growth factor-binding protein 5 1 shared papers
• Heterogeneous nuclear ribonucleoprotein D0 2 shared papers
• 28S rRNA/ribosome and sororin micro-cofactor 1 shared papers
• Nuclear valosin-containing protein-like 1 shared papers
• Heterogeneous nuclear ribonucleoprotein U 4 shared papers
• Midkine 1 shared papers
• 60S ribosome subunit biogenesis protein NIP7 homolog 1 shared papers
• Nucleophosmin 4 shared papers
• ATP-dependent RNA helicase DDX24 1 shared papers
• Telomerase reverse transcriptase 1 shared papers
• Hepatoma-derived growth factor 1 shared papers

Literature · 48 cited papers

• Identification of Microprotein-Protein Interactions via APEX Tagging. Biochemistry · 2017
• Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation. Nat. Struct. Mol. Biol. · 2017
• Two new isoforms of the human hepatoma-derived growth factor interact with components of the cytoskeleton. Biol. Chem. · 2016
• IGFBP-5 Promotes Fibrosis Independently of Its Translocation to the Nucleus and Its Interaction with Nucleolin and IGF. PLoS ONE · 2015
• N-terminome analysis of the human mitochondrial proteome. Proteomics · 2015
• Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli. Proc. Natl. Acad. Sci. U.S.A. · 2014
• MDM2 mediates nonproteolytic polyubiquitylation of the DEAD-Box RNA helicase DDX24. Mol. Cell. Biol. · 2014
• Subcellular fractionation and localization studies reveal a direct interaction of the fragile X mental retardation protein (FMRP) with nucleolin. PLoS ONE · 2014
• An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
• Immunoaffinity enrichment and mass spectrometry analysis of protein methylation. Mol. Cell. Proteomics · 2014
• ABH2 couples regulation of ribosomal DNA transcription with DNA alkylation repair. Cell Rep. · 2013
• Nucleolar scaffold protein, WDR46, determines the granular compartmental localization of nucleolin and DDX21. Genes Cells · 2013
• … and 36 more in the literature graph