Serine/threonine protein kinase UL97

Function

Serine/threonine protein kinase that plays important roles in several processes including nuclear viral egress, viral replication or regulation of host cell cycle progression. Participates in the acquisition of tegument during virion morphogenesis in the nucleus. Phosphorylates the viral nuclear egress complex (NEC) subunits UL50 and UL53. Redistributes the host nuclear lamina by phosphorylating cellular Lamins-A/C. Plays a role in viral DNA synthesis by phosphorylating the DNA polymerase processivity factor UL44. Stimulates host cell cycle to support viral DNA synthesis by phosphorylating host retinoblastoma/RB1 protein. Additional substrates have been identified including host EF1D or H2B. Also phosphorylates host SAMHD1 and thereby counteracts its antiviral effect by reducing its dNTP hydrolase activity.

Classification

Family (Pfam)

PF06734 UL97

InterPro

Herpes_UL97, Kinase-like_dom_sf, Tyr_kinase_AS

Functional cluster

Viral Structural & Chromatin Proteins

Gene Ontology · 6

• GO:0044423 virion component
• GO:0005524 ATP binding
• GO:0106310 protein serine kinase activity
• GO:0004674 protein serine/threonine kinase activity
• GO:0044071 symbiont-mediated perturbation of host cell cycle progression
• GO:0016032 viral process

Drugs targeting this protein · 1

• MARIBAVIR inhibitor

Related proteins · sequence + function similarity

• Serine/threonine protein kinase UL97 1.00
• Serine/threonine protein kinase UL97 1.00
• Phosphoprotein 85 0.75
• Phosphoprotein 85 0.75
• Serine/threonine protein kinase M97 0.74
• mRNA export factor ICP27 homolog 0.74
• Serine/threonine-protein kinase UL13 0.72
• Protein UL87 0.72
• Uncharacterized protein UL87 0.71
• Viral IRF3-like protein 0.70
• DNA helicase/primase complex-associated protein 0.69
• DNA helicase/primase complex-associated protein 0.69

Co-cited proteins · studied together in the literature

• Serine/threonine protein kinase UL97 1 shared papers
• Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 2 shared papers
• Nuclear egress protein 2 1 shared papers
• Nuclear egress protein 1 1 shared papers
• Viral protein kinase 1 shared papers
• Serine/threonine-protein kinase BGLF4 1 shared papers

Literature · 20 cited papers

• Human cytomegalovirus overcomes SAMHD1 restriction in macrophages via pUL97. Nat. Microbiol. · 2019
• Conserved Herpesvirus Protein Kinases Target SAMHD1 to Facilitate Virus Replication. Cell Rep. · 2019
• Human cytomegalovirus UL97 phosphorylates the viral nuclear egress complex. J. Virol. · 2015
• Human cytomegalovirus UL97 kinase and nonkinase functions mediate viral cytoplasmic secondary envelopment. J. Virol. · 2011
• Phosphorylation of retinoblastoma protein by viral protein with cyclin-dependent kinase function. Science · 2008
• Human cytomegalovirus protein kinase UL97 forms a complex with the tegument phosphoprotein pp65. J. Virol. · 2007
• Human cytomegalovirus UL97 Kinase is required for the normal intranuclear distribution of pp65 and virion morphogenesis. J. Virol. · 2005
• Identification of proteins in human cytomegalovirus (HCMV) particles: the HCMV proteome. J. Virol. · 2004
• Phosphorylation of the RNA polymerase II carboxyl-terminal domain in human cytomegalovirus-infected cells and in vitro by the viral UL97 protein kinase. Virology · 2004
• The human cytomegalovirus UL44 protein is a substrate for the UL97 protein kinase. J. Virol. · 2003
• The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome. J. Gen. Virol. · 2003
• The human cytomegalovirus UL97 protein kinase, an antiviral drug target, is required at the stage of nuclear egress. J. Virol. · 2003
• … and 8 more in the literature graph