Mating-type protein ALPHA2

Function

Mating type proteins are sequence specific DNA-binding proteins that act as master switches in yeast differentiation by controlling gene expression in a cell type-specific fashion. Transcriptional corepressor that binds cooperatively with MCM1 to a 31-basepair DNA sequence termed the a-specific gene (asg) operator, to repress the transcription of a-cell-specific genes. Additionally, in a/alpha diploid cells, binds cooperatively with the A1 protein to a 21-basepair DNA sequence termed the haploid-specific gene (hsg) operator, to repress transcription of haploid-specific genes and of MATALPHA1.

Classification

Family (Pfam)

PF00046 Homeodomain

InterPro

HD, Homeodomain-like_sf

Functional cluster

Nuclear Transport & RNA-Processing Proteins

Experimental structures · PDB · 6

• 1AKH X-ray 2.50A
• 1APL X-ray 2.70A
• 1K61 X-ray 2.10A
• 1LE8 X-ray 2.30A
• 1MNM X-ray 2.25A
• 1YRN X-ray 2.50A

A predicted model is available from AlphaFold.

Gene Ontology · 8

• GO:0005634 nucleus
• GO:0008301 DNA binding, bending
• GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific
• GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
• GO:0043565 sequence-specific DNA binding
• GO:0006351 DNA-templated transcription
• GO:0000122 negative regulation of transcription by RNA polymerase II
• GO:0007532 regulation of mating-type specific transcription, DNA-templated

Neighborhood · nearest proteins

Related proteins · sequence + function similarity

• Silenced mating-type protein ALPHA2 1.00
• Mating-type protein ALPHA2 0.86
• Mating-type-like protein ALPHA2 0.86
• Mating-type protein ALPHA2 0.82
• Silenced mating-type protein A2 0.81
• Putative mating-type protein A2 0.81
• Mating-type-like protein ALPHA2 0.79
• Mating-type-like protein ALPHA2, silenced copy at MTL3 0.72
• Mating-type protein A1 0.71
• Silenced mating-type protein A1 0.71
• Homeobox protein HD-12 0.71
• Mating-type protein A1 0.70

Co-cited proteins · studied together in the literature

• Mating-type protein A1 6 shared papers
• Pheromone receptor transcription factor 2 shared papers
• General transcriptional corepressor CYC8 2 shared papers
• Mating-type protein ALPHA1 3 shared papers
• ERAD-associated E3 ubiquitin-protein ligase DOA10 1 shared papers
• General transcriptional corepressor TUP1 1 shared papers
• Uncharacterized protein YCR043C 1 shared papers
• Putative mating-type protein A2 1 shared papers
• Bud site selection protein 5 1 shared papers
• Transcription initiation factor TFIID subunit 2 1 shared papers
• Silenced mating-type protein ALPHA1 1 shared papers
• Silenced mating-type protein ALPHA2 1 shared papers

Literature · 23 cited papers

• The reference genome sequence of Saccharomyces cerevisiae: Then and now. G3 (Bethesda) · 2014
• N-terminal acetylation of cellular proteins creates specific degradation signals. Science · 2010
• Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae. Genome Res. · 2007
• A Hoogsteen base pair embedded in undistorted B-DNA. Nucleic Acids Res. · 2002
• Structural and thermodynamic characterization of the DNA binding properties of a triple alanine mutant of MATalpha2. Structure · 2002
• A sequence resembling a peroxisomal targeting sequence directs the interaction between the tetratricopeptide repeats of Ssn6 and the homeodomain of alpha 2. Proc. Natl. Acad. Sci. U.S.A. · 2000
• Crystal structure of the MATa1/MATalpha2 homeodomain heterodimer in complex with DNA containing an A-tract. Nucleic Acids Res. · 1998
• Crystal structure of the yeast MATalpha2/MCM1/DNA ternary complex. Nature · 1998
• Molecular mechanisms of cell-type determination in budding yeast. Curr. Opin. Genet. Dev. · 1995
• The yeast alpha2 and Mcm1 proteins interact through a region similar to a motif found in homeodomain proteins of higher eukaryotes. Mol. Cell. Biol. · 1996
• The WD repeats of Tup1 interact with the homeo domain protein alpha 2. Genes Dev. · 1994
• Crystal structure of the MATa1/MAT alpha 2 homeodomain heterodimer bound to DNA. Science · 1995
• … and 11 more in the literature graph