Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

UniProt P0AEK4 Organism Escherichia coli (strain K12) Gene JW1281, b1288, envM, fabI EC 1.3.1.9

Also known as: JW1281, b1288, envM, fabI

Function

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis.

Classification

Family (Pfam): PF13561 adh_short_C2
InterPro: Enoyl-ACP_Rdtase_NADH, NAD(P)-bd_dom_sf, SDR_fam
Functional cluster: RuBisCO & Carbon-Fixation Enzymes

Experimental structures · PDB · 23

1C14 X-ray 2.00A
1D8A X-ray 2.20A
1DFG X-ray 2.50A
1DFH X-ray 2.20A
1DFI X-ray 2.09A
1I2Z X-ray 2.80A
1I30 X-ray 2.40A
1LX6 X-ray 2.40A
1LXC X-ray 2.40A
1MFP X-ray 2.33A
1QG6 X-ray 1.90A
1QSG X-ray 1.75A
… and 11 more

A predicted model is available from AlphaFold.

Gene Ontology · 12

Drugs targeting this protein · 2

HEXACHLOROPHENE inhibitor
TRICLOSAN inhibitor

Related proteins · sequence + function similarity

Co-cited proteins · studied together in the literature

Uncharacterized protein YcjD 2 shared papers
Enoyl-[acyl-carrier-protein] reductase [NADPH] FabI 3 shared papers
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI 2 shared papers
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI 1 shared papers
3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase 3 shared papers
3-oxoacyl-[acyl-carrier-protein] reductase FabG 3 shared papers
Beta-ketoacyl-[acyl-carrier-protein] synthase III 3 shared papers
Enoyl-[acyl-carrier-protein] reductase [NADPH] FabL 1 shared papers
8-amino-7-oxononanoate synthase 1 shared papers
Malonyl-[acyl-carrier protein] O-methyltransferase 1 shared papers
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ 2 shared papers
Pimeloyl-[acyl-carrier protein] methyl ester esterase 1 shared papers

Literature · 23 cited papers

Biotin synthesis begins by hijacking the fatty acid synthetic pathway. Nat. Chem. Biol. · 2010
Novel enoyl-ACP reductase (FabI) potential inhibitors of Escherichia coli from Chinese medicine monomers. Bioorg. Med. Chem. Lett. · 2010
Structure of acyl carrier protein bound to FabI, the FASII enoyl reductase from Escherichia coli. J. Biol. Chem. · 2006
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol. Syst. Biol. · 2006
Indole naphthyridinones as inhibitors of bacterial enoyl-ACP reductases FabI and FabK. J. Med. Chem. · 2003
Discovery of aminopyridine-based inhibitors of bacterial enoyl-ACP reductase (FabI). J. Med. Chem. · 2002
1,4-Disubstituted imidazoles are potential antibacterial agents functioning as inhibitors of enoyl acyl carrier protein reductase (FabI). Bioorg. Med. Chem. Lett. · 2001
The enoyl-[acyl-carrier-protein] reductases FabI and FabL from Bacillus subtilis. J. Biol. Chem. · 2000
Beta-ketoacyl-acyl carrier protein synthase III (FabH) is a determining factor in branched-chain fatty acid biosynthesis. J. Bacteriol. · 2000
Molecular basis for triclosan activity involves a flipping loop in the active site. Protein Sci. · 1999
Kinetic and structural characteristics of the inhibition of enoyl (acyl carrier protein) reductase by triclosan. Biochemistry · 1999
Structural basis and mechanism of enoyl reductase inhibition by triclosan. J. Mol. Biol. · 1999
… and 11 more in the literature graph

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