Dihydropteroate synthase

Function

Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives.

Classification

Family (Pfam)

PF00809 Pterin_bind

InterPro

DHP_synth-like, DHP_synth_dom, DHPS, Dihydropteroate_synth-like_sf, Pterin-binding_dom

Functional cluster

Amino Acid & Nucleotide Biosynthesis Enzymes

Experimental structures · PDB · 17

• 1AJ0 X-ray 2.00A
• 1AJ2 X-ray 2.00A
• 1AJZ X-ray 2.00A
• 5U0V X-ray 1.70A
• 5U0W X-ray 1.97A
• 5U0Y X-ray 1.88A
• 5U0Z X-ray 2.29A
• 5U10 X-ray 2.04A
• 5U11 X-ray 1.99A
• 5U12 X-ray 1.84A
• 5U13 X-ray 1.95A
• 5U14 X-ray 1.95A
• … and 5 more

A predicted model is available from AlphaFold.

Gene Ontology · 7

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0004156 dihydropteroate synthase activity
• GO:0046872 metal ion binding
• GO:0046656 folic acid biosynthetic process
• GO:0009410 response to xenobiotic stimulus
• GO:0046654 tetrahydrofolate biosynthetic process

Drugs targeting this protein · 23

• SULFAPHENAZOLE inhibitor
• SULFAMETHIZOLE inhibitor
• SULFISOXAZOLE DIOLAMINE inhibitor
• SULFADIAZINE SODIUM inhibitor
• SULFAMETER inhibitor
• SULFISOXAZOLE ACETYL inhibitor
• MAFENIDE ACETATE inhibitor
• SULFADIAZINE, SILVER inhibitor
• SULFALENE inhibitor
• SULFACETAMIDE SODIUM inhibitor
• SULFANILAMIDE inhibitor
• SULFADIMETHOXINE SODIUM inhibitor
• SULFAMETHOXYPYRIDAZINE inhibitor
• SULFATHIAZOLE inhibitor
• SULFAMERAZINE inhibitor
• SULFADIAZINE inhibitor
• SULFAMETHOXAZOLE inhibitor
• SULFAMETHAZINE inhibitor
• SULFISOXAZOLE inhibitor
• SULFACETAMIDE inhibitor
• CO-TRIMOXAZOLE inhibitor
• SULFADIMETHOXINE inhibitor
• SULFAPYRIDINE inhibitor

Related proteins · sequence + function similarity

• Dihydropteroate synthase 1.00
• Dihydropteroate synthase 1.00
• Dihydropteroate synthase 0.86
• Dihydropteroate synthase 0.83
• Dihydropteroate synthase 0.81
• Dihydropteroate synthase 0.79
• Dihydropteroate synthase 0.72
• Dihydropteroate synthase 0.72
• Dihydropteroate synthase 0.70
• Dihydropteroate synthase 0.69
• Dihydropteroate synthase 0.69
• Dihydropteroate synthase 0.69

Co-cited proteins · studied together in the literature

• Dihydropteroate synthase 1 shared papers
• 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase 1 shared papers
• Dihydropteroate synthase type-3 1 shared papers
• Dihydropteroate synthase type-2 1 shared papers
• Dihydropteroate synthase type-1 1 shared papers

Literature · 10 cited papers

• Molecular mechanism of plasmid-borne resistance to sulfonamide antibiotics. Nat. Commun. · 2023
• 8-Mercaptoguanine Derivatives as Inhibitors of Dihydropteroate Synthase. Chemistry · 2018
• Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol. Syst. Biol. · 2006
• The complete genome sequence of Escherichia coli K-12. Science · 1997
• Crystal structure of the anti-bacterial sulfonamide drug target dihydropteroate synthase. Nat. Struct. Biol. · 1997
• Point mutations in the dihydropteroate synthase gene causing sulfonamide resistance. Adv. Exp. Med. Biol. · 1993
• The biosynthesis of folic acid. IX. Purification and properties of the enzymes required for the formation of dihydropteroic acid. J. Biol. Chem. · 1969
• Purification and partial characterization of 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase and 7,8-dihydropteroate synthase from Escherichia coli MC4100. J. Bacteriol. · 1991
• Cloning, sequencing, and enhanced expression of the dihydropteroate synthase gene of Escherichia coli MC4100. J. Bacteriol. · 1992
• Characterization of mutationally altered dihydropteroate synthase and its ability to form a sulfonamide-containing dihydrofolate analog. J. Bacteriol. · 1979