UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Function

Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine. Target for the antibiotic fosfomycin.

Classification

Family (Pfam)

PF00275 EPSP_synthase

InterPro

Enolpyruvate_Tfrase_dom, Enolpyruvate_Tfrase_sf, RNA3'P_cycl/enolpyr_Trfase_a/b, UDP_GlcNAc_COvinyl_MurA

Functional cluster

Amino-Acid & Central Metabolism Enzymes

Experimental structures · PDB · 8

• 1A2N X-ray 2.80A
• 1UAE X-ray 1.80A
• 2Z2C X-ray 2.05A
• 3ISS X-ray 2.50A
• 3KQJ X-ray 1.70A
• 3KR6 X-ray 1.70A
• 3SWD X-ray 2.50A
• 5VM7 EM 5.70A

A predicted model is available from AlphaFold.

Gene Ontology · 7

• GO:0005829 cytosol
• GO:0008760 UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity
• GO:0051301 cell division
• GO:0071555 cell wall organization
• GO:0009252 peptidoglycan biosynthetic process
• GO:0008360 regulation of cell shape
• GO:0019277 UDP-N-acetylgalactosamine biosynthetic process

Drugs targeting this protein · 1

• FOSFOMYCIN TROMETHAMINE inhibitor

Related proteins · sequence + function similarity

• UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1.00
• UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1.00
• UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1.00
• UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1.00
• UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1.00
• UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1.00
• UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1.00
• UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1.00
• UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1.00
• UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1.00
• UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1.00
• UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1.00

Co-cited proteins · studied together in the literature

• UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 shared papers
• UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 shared papers
• UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 shared papers
• Antitoxin PezA 1 shared papers
• Toxin PezT 1 shared papers

Literature · 9 cited papers

• Functional consequence of covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA). J. Biol. Chem. · 2012
• A novel mechanism of programmed cell death in bacteria by toxin-antitoxin systems corrupts peptidoglycan synthesis. PLoS Biol. · 2011
• The fungal product terreic acid is a covalent inhibitor of the bacterial cell wall biosynthetic enzyme UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA). Biochemistry · 2010
• Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol. Syst. Biol. · 2006
• Emergence of fosfomycin-resistant isolates of Shiga-like toxin-producing Escherichia coli O26. Antimicrob. Agents Chemother. · 1999
• Stereochemical course of enzymatic enolpyruvyl transfer and catalytic conformation of the active site revealed by the crystal structure of the fluorinated analogue of the reaction tetrahedral intermediate bound to the active site of the C115A mutant of MurA. Biochemistry · 1998
• The complete genome sequence of Escherichia coli K-12. Science · 1997
• Structure of UDP-N-acetylglucosamine enolpyruvyl transferase, an enzyme essential for the synthesis of bacterial peptidoglycan, complexed with substrate UDP-N-acetylglucosamine and the drug fosfomycin. Structure · 1996
• Cloning and sequencing of Escherichia coli murZ and purification of its product, a UDP-N-acetylglucosamine enolpyruvyl transferase. J. Bacteriol. · 1992