Purine nucleoside phosphorylase

Function

Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine.

Classification

Family (Pfam)

PF01048 PNP_UDP_1

InterPro

Nucleoside_phosphorylase_d, Nucleoside_phosphorylase_sf, Pur_Nuc_Pase_Ino/Guo-sp, Purine_phosphorylase, Purine_phosphorylase-2_CS

Functional cluster

Amino Acid & Cofactor Biosynthesis Enzymes

Experimental structures · PDB · 41

• 1M73 X-ray 2.30A
• 1PF7 X-ray 2.60A
• 1PWY X-ray 2.80A
• 1RCT X-ray 2.80A
• 1RFG X-ray 2.90A
• 1RR6 X-ray 2.50A
• 1RSZ X-ray 2.20A
• 1RT9 X-ray 2.30A
• 1ULA X-ray 2.75A
• 1ULB X-ray 2.75A
• 1V2H X-ray 2.70A
• 1V3Q X-ray 2.80A
• … and 29 more

A predicted model is available from AlphaFold.

Gene Ontology · 29

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0070062 extracellular exosome
• GO:0005576 extracellular region
• GO:1904813 ficolin-1-rich granule lumen
• GO:0034774 secretory granule lumen
• GO:0047975 guanosine phosphorylase activity
• GO:0042802 identical protein binding
• GO:0001882 nucleoside binding
• GO:0042301 phosphate ion binding
• GO:0002060 purine nucleobase binding
• GO:0004731 purine-nucleoside phosphorylase activity
• GO:0000255 allantoin metabolic process
• GO:0046059 dAMP catabolic process
• GO:0006157 deoxyadenosine catabolic process
• GO:0006149 deoxyinosine catabolic process
• GO:0006955 immune response
• GO:0006204 IMP catabolic process
• GO:0006148 inosine catabolic process
• GO:0006738 nicotinamide riboside catabolic process
• GO:0006139 nucleobase-containing compound metabolic process
• GO:0009165 nucleotide biosynthetic process
• GO:0046638 positive regulation of alpha-beta T cell differentiation
• GO:0032743 positive regulation of interleukin-2 production
• GO:0042102 positive regulation of T cell proliferation
• GO:0006166 purine ribonucleoside salvage
• GO:0043101 purine-containing compound salvage
• GO:0009410 response to xenobiotic stimulus
• GO:0034418 urate biosynthetic process

Disease associations

• purine nucleoside phosphorylase deficiency MONDO:0013171

Drugs targeting this protein · 2

• ULODESINE inhibitor
• FORODESINE HYDROCHLORIDE inhibitor

Related proteins · sequence + function similarity

• Purine nucleoside phosphorylase 0.98
• Purine nucleoside phosphorylase 0.98
• Purine nucleoside phosphorylase 0.97
• Purine nucleoside phosphorylase 1 0.88
• Purine nucleoside phosphorylase 1 0.87
• Purine nucleoside phosphorylase 2 0.85
• Purine nucleoside phosphorylase 0.67
• Putative purine nucleoside phosphorylase 0.67
• Purine nucleoside phosphorylase 0.66
• Uridine phosphorylase 0.61
• Uridine phosphorylase 0.61
• 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 0.61

Co-cited proteins · studied together in the literature

• Glutathione S-transferase omega-1 1 shared papers
• Fumarate hydratase, mitochondrial 1 shared papers
• Exportin-7 1 shared papers

Literature · 19 cited papers

• N-terminome analysis of the human mitochondrial proteome. Proteomics · 2015
• An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
• Catalytic site conformations in human PNP by 19F-NMR and crystallography. Chem. Biol. · 2013
• Toward a comprehensive characterization of a human cancer cell phosphoproteome. J. Proteome Res. · 2013
• Assessment of the red cell proteome of young patients with unexplained hemolytic anemia by two-dimensional differential in-gel electrophoresis (DIGE). PLoS ONE · 2012
• Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features. Mol. Cell. Proteomics · 2012
• Initial characterization of the human central proteome. BMC Syst. Biol. · 2011
• Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal. Chem. · 2009
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. · 2004
• Structures of human purine nucleoside phosphorylase complexed with inosine and ddI. Biochem. Biophys. Res. Commun. · 2004
• Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat. Genet. · 2004
• Genetic variation in genes associated with arsenic metabolism: glutathione S-transferase omega 1-1 and purine nucleoside phosphorylase polymorphisms in European and indigenous Americans. Environ. Health Perspect. · 2003
• … and 7 more in the literature graph