Free fatty acid receptor 2
Also known as: FFA2, FFAR2, GPCR43, GPR43
Function
G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, the short chain fatty acids acetate, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins but also to the Gq family. Its activation results in the formation of inositol 1,4,5-trisphosphate, the mobilization of intracellular calcium, the phosphorylation of the MAPK3/ERK1 and MAPK1/ERK2 kinases and the inhibition of intracellular cAMP accumulation. May play a role in glucose homeostasis by regulating the secretion of GLP-1, in response to short-chain fatty acids accumulating in the intestine. May also regulate the production of LEP/Leptin, a hormone acting on the central nervous system to inhibit food intake. Finally, may also regulate whole-body energy homeostasis through adipogenesis regulating both differentiation and lipid storage of adipocytes. In parallel to its role in energy homeostasis, may also mediate the activation of the inflammatory and immune responses by SCFA in the intestine, regulating the rapid production of chemokines and cytokines. May also play a role in the resolution of the inflammatory response and control chemotaxis in neutrophils. In addition to SCFAs, may also be activated by the extracellular lectin FCN1 in a process leading to activation of monocytes and inducing the secretion of interleukin-8/IL-8 in response to the presence of microbes. Among SCFAs, the fatty acids containing less than 6 carbons, the most potent activators are probably acetate, propionate and butyrate. Exhibits a SCFA-independent constitutive G protein-coupled receptor activity.
Classification
- Family (Pfam)
- PF00001 7tm_1
- InterPro
- GPCR_Rhodpsn, GPCR_Rhodpsn_7TM, GPR40-rel_orph
- Functional cluster
- G Protein-Coupled Receptors
Experimental structures · PDB · 11
- 8J22 EM 3.20A
- 8J23 EM 3.20A
- 8J24 EM 2.60A
- 8T3S EM 3.07A
- 8Y6W EM 3.19A
- 8Y6Y EM 3.36A
- 9CLW EM 3.19A
- 9CM3 EM 3.06A
- 9CM7 EM 3.29A
- 9K1D EM 3.34A
- 9NS9 EM 3.30A
A predicted model is available from AlphaFold.
Gene Ontology · 19
- GO:0042995 cell projection
- GO:0005886 plasma membrane
- GO:0004930 G protein-coupled receptor activity
- GO:0008289 lipid binding
- GO:0002752 cell surface pattern recognition receptor signaling pathway
- GO:0071398 cellular response to fatty acid
- GO:0045444 fat cell differentiation
- GO:0007186 G protein-coupled receptor signaling pathway
- GO:0042593 glucose homeostasis
- GO:0002232 leukocyte chemotaxis involved in inflammatory response
- GO:0019915 lipid storage
- GO:0002385 mucosal immune response
- GO:0007200 phospholipase C-activating G protein-coupled receptor signaling pathway
- GO:0002879 positive regulation of acute inflammatory response to non-antigenic stimulus
- GO:0032722 positive regulation of chemokine production
- GO:0002720 positive regulation of cytokine production involved in immune response
- GO:0032757 positive regulation of interleukin-8 production
- GO:0002673 regulation of acute inflammatory response
- GO:0090276 regulation of peptide hormone secretion
Drugs targeting this protein · 1
- GLPG-0974 antagonist
Related proteins · sequence + function similarity
- Free fatty acid receptor 2 0.99
- Free fatty acid receptor 2 0.99
- Free fatty acid receptor 3 0.96
- Free fatty acid receptor 3 0.96
- G protein-coupled receptor 42 0.95
- Free fatty acid receptor 3 0.95
- S-geranylgeranyl-glutathione receptor P2RY8 0.92
- G protein-coupled receptor 55 0.92
- G protein-coupled receptor 55 0.90
- Uracil nucleotide/cysteinyl leukotriene receptor 0.90
- Uracil nucleotide/cysteinyl leukotriene receptor 0.90
- G protein-coupled receptor 35 0.90
Co-cited proteins · studied together in the literature
- Free fatty acid receptor 3 5 shared papers
- Free fatty acid receptor 2 3 shared papers
- Ficolin-1 1 shared papers
- Free fatty acid receptor 2 1 shared papers
- G protein-coupled receptor 42 2 shared papers
- C-reactive protein 1 shared papers
- Free fatty acid receptor 3 1 shared papers
- Free fatty acid receptor 1 1 shared papers
- Free fatty acid receptor 3 1 shared papers
Literature · 12 cited papers
- Defining the molecular basis for the first potent and selective orthosteric agonists of the FFA2 free fatty acid receptor. J. Biol. Chem. · 2013
- Extracellular ionic locks determine variation in constitutive activity and ligand potency between species orthologs of the free fatty acid receptors FFA2 and FFA3. J. Biol. Chem. · 2012
- Mutational analysis of G-protein coupled receptor--FFA2. Biochem. Biophys. Res. Commun. · 2011
- Secreted M-ficolin anchors onto monocyte transmembrane G protein-coupled receptor 43 and cross talks with plasma C-reactive protein to mediate immune signaling and regulate host defense. J. Immunol. · 2010
- Allosteric rescuing of loss-of-function FFAR2 mutations. FEBS Lett. · 2010
- Conserved polar residues in transmembrane domains V, VI, and VII of free fatty acid receptor 2 and free fatty acid receptor 3 are required for the binding and function of short chain fatty acids. J. Biol. Chem. · 2008
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. · 2004
- The DNA sequence and biology of human chromosome 19. Nature · 2004
- Functional characterization of human receptors for short chain fatty acids and their role in polymorphonuclear cell activation. J. Biol. Chem. · 2003
- Identification of a free fatty acid receptor, FFA2R, expressed on leukocytes and activated by short-chain fatty acids. Biochem. Biophys. Res. Commun. · 2003
- The orphan G protein-coupled receptors GPR41 and GPR43 are activated by propionate and other short chain carboxylic acids. J. Biol. Chem. · 2003
- A cluster of four novel human G protein-coupled receptor genes occurring in close proximity to CD22 gene on chromosome 19q13.1. Biochem. Biophys. Res. Commun. · 1997